Anti-PPM1K抗体(ab224424)
Key features and details
- Rabbit polyclonal to PPM1K
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-PPM1K抗体
参阅全部 PPM1K 一抗 -
描述
兔多克隆抗体to PPM1K -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Cow
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免疫原
Recombinant fragment corresponding to Human PPM1K aa 250 to the C-terminus.
Database link: Q8N3J5 -
阳性对照
- WB: PPM1K overexpression HEK-293T cell lysate. IHC-P: Human urinary bladder tissue. ICC/IF: U-2 OS cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab224424于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/100 - 1/250. Predicted molecular weight: 41 kDa.
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| IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
| 说明 |
|---|
|
WB
1/100 - 1/250. Predicted molecular weight: 41 kDa. |
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IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
靶标
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功能
Regulates the mitochondrial permeability transition pore and is essential for cellular survival and development. -
序列相似性
Belongs to the PP2C family.
Contains 1 PP2C-like domain. -
细胞定位
Mitochondrion matrix. - Information by UniProt
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数据库链接
- Entrez Gene: 152926 Human
- Entrez Gene: 243382 Mouse
- Omim: 611065 Human
- SwissProt: Q2PC20 Cow
- SwissProt: Q8N3J5 Human
- SwissProt: Q8BXN7 Mouse
- Unigene: 291000 Human
- Unigene: 709966 Human
see all -
别名
- BDP antibody
- mitochondrial antibody
- MSUDMV antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-PPM1K antibody (ab224424)
PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for PPM1K (green) using ab224424 at 4 µg/ml in ICC/IF.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPM1K antibody (ab224424)Paraffin-embedded human urinary bladder tissue stained for PPM1K using ab224424 at 1/200 dilution in immunohistochemical analysis.
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Western blot - Anti-PPM1K antibody (ab224424)All lanes : Anti-PPM1K antibody (ab224424) at 1/100 dilution
Lane 1 : Vector only transfected HEK-293T (human epithelial cell line from embryonic kidney transformed wth a large T antigen) cell lysate
Lane 2 : PPM1K overexpression HEK-293T cell lysate (Co-expressed with a C-terminal myc-DDK tag, ~3.1 kDa)
Predicted band size: 41 kDa
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab224424 尚未被引用在任何文献中。