AB186423

重组Anti-PPM1G抗体[EPR13362-31]

Anti-PPM1G antibody [EPR13362-31]

RabMAb
Recombinant
了解详情

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(1 Publication)

Rabbit Recombinant Monoclonal PPM1G antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse samples. Cited in 1 publication.

查看别名

PPM1C, PPM1G, Protein phosphatase 1G, Protein phosphatase 1C, Protein phosphatase 2C isoform gamma, Protein phosphatase magnesium-dependent 1 gamma, PP2C-gamma

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPM1G antibody [EPR13362-31] (AB186423)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPM1G antibody [EPR13362-31] (AB186423)

Immunohistochemical analysis of paraffin-embedded Human kidney clear cell carcinoma tissue labeling PPM1G with ab186423 at 1/500 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-PPM1G antibody [EPR13362-31] (AB186423)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PPM1G antibody [EPR13362-31] (AB186423)

Immunofluorescent analysis of 4% paraformaldehyde-fixed 293T cells labeling PPM1G with ab186423 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution. Counter stained with Dapi.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PPM1G antibody [EPR13362-31] (AB186423)

Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling PPM1G with ab186423 at 1/500 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody at 1/200 dilution (Green). Counter stained with Dapi (Blue).

Flow Cytometry (Intracellular) - Anti-PPM1G antibody [EPR13362-31] (AB186423)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-PPM1G antibody [EPR13362-31] (AB186423)

Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed 293 cells labeling PPM1G with ab186423 at 1/200 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPM1G antibody [EPR13362-31] (AB186423)

Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labeling PPM1G with ab186423 at 1/500 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-PPM1G antibody [EPR13362-31] (AB186423)

All lanes:

Western blot - Anti-PPM1G antibody [EPR13362-31] (ab186423) at 1/10000 dilution

Lane 1:

293T cell lysate at 20 µg

Lane 2:

Jurkat cell lysate at 20 µg

Lane 3:

HeLa cell lysate at 20 µg

Lane 4:

MCF7 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 59 kDa

false

不同偶联物与剂型 (1)

Carrier free

Anti-PPM1G antibody [EPR13362-31] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR13362-31

亚型

IgG

不含载体蛋白

反应种属

Mouse, Human

应用

ICC/IF, Flow Cyt (Intra), WB, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/200", "FlowCytIntra-species-notes": "<a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

PPM1G also known as protein phosphatase 1G is a member of the protein phosphatase 2C (PP2C) family. This enzyme has a molecular mass of approximately 58 kDa. It localizes mainly in the nucleus and is expressed in various tissues including the brain testis and skeletal muscle. PPM1G functions as a serine/threonine phosphatase removing phosphate groups from target proteins and playing a role in the regulation of cellular stress responses and cell cycle progression.

Biological function summary

PPM1G contributes to several cellular functions by interacting with different substrates. As a component of the cellular machinery it is involved in processes such as RNA processing and regulation of mitosis. The enzyme participates in dephosphorylation events critical for the assembly and disassembly of the spliceosome complex which is important for accurate mRNA splicing. PPM1G's activity helps maintain the proper function of these cellular processes ensuring the stability and output of gene expression.

Pathways

Several significant cellular activities are influenced by PPM1G's regulatory mechanisms. It is implicated in the mitogen-activated protein kinase (MAPK) signaling pathway and the cell cycle regulation pathway. These pathways are vital for controlling cell growth and division. In these contexts PPM1G interacts with various other proteins such as p53 and cyclin-dependent kinases (CDKs) linking it to cell cycle checkpoints and stress responses. These interactions allow PPM1G to regulate and coordinate cellular responses to external and internal signals.

PPM1G has connections with illnesses like cancer and neurodegenerative diseases. In cancer its role in cell cycle regulation can affect tumor growth and progression often depending on its interactions with proteins like CDK. In neurodegenerative disorders PPM1G might play a part due to its involvement in RNA processing and protein dephosphorylation processes which are critical for neuron function. Any dysregulation in these interactions may contribute to disease pathology making PPM1G a point of interest for understanding and potentially treating these conditions.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

See full target information PPM1G

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Animals : an open access journal from MDPI 15: PubMed40804973

2025

Intestinal Alkaline Phosphatase Expression in Response to Infection in Nursery Pigs.

Applications

Unspecified application

Species

Unspecified reactive species

Sireethon Maksin,Attapon Kamlangdee,Alongkot Boonsoongnern,Prapassorn Boonsoongnern

View all publications

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详情请参阅我们的条款与条件。

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重组Anti-PPM1G抗体[EPR13362-31]