Anti-PPM1D/WIP1抗体(ab31270)
Key features and details
- Rabbit polyclonal to PPM1D/WIP1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-PPM1D/WIP1抗体
参阅全部 PPM1D/WIP1 一抗 -
描述
兔多克隆抗体to PPM1D/WIP1 -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Xenopus laevis, Zebrafish
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免疫原
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阳性对照
- This antibody gave a positive signal in the following whole cell lysates: Jurkat and HEK293 in WB.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab31270于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 67 kDa (predicted molecular weight: 67 kDa).
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| 说明 |
|---|
|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 67 kDa (predicted molecular weight: 67 kDa). |
靶标
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功能
Required for the relief of p53-dependent checkpoint mediated cell cycle arrest. Binds to and dephosphorylates 'Ser-15' of TP53 and 'Ser-345' of CHEK1 which contributes to the functional inactivation of these proteins. -
序列相似性
Belongs to the PP2C family.
Contains 1 PP2C-like domain. - Information by UniProt
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数据库链接
- Entrez Gene: 8493 Human
- Entrez Gene: 53892 Mouse
- Entrez Gene: 380383 Xenopus laevis
- Entrez Gene: 394065 Zebrafish
- Omim: 605100 Human
- SwissProt: O15297 Human
- SwissProt: Q9QZ67 Mouse
- Unigene: 286073 Human
see all -
别名
- EC 3.1.3.16 antibody
- p53 induced protein phosphatase 1 antibody
- p53-induced protein phosphatase 1 antibody
see all
图片
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Western blot - Anti-PPM1D/WIP1 antibody (ab31270)All lanes : Anti-PPM1D/WIP1 antibody (ab31270) at 1 µg/ml
Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 67 kDa
Observed band size: 67 kDa
Exposure time: 20 minutes
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (11)
ab31270 被引用在 11 文献中.
- Li G et al. Wip1 contributes to the adaptation of HepG2 human liver cancer cells to stress hormone-induced DNA damage. Oncol Lett 25:31 (2023). PubMed: 36589663
- Tokheim C et al. Systematic characterization of mutations altering protein degradation in human cancers. Mol Cell 81:1292-1308.e11 (2021). PubMed: 33567269
- Deng K et al. WIP1 promotes cancer stem cell properties by inhibiting p38 MAPK in NSCLC. Signal Transduct Target Ther 5:36 (2020). PubMed: 32296033
- Lu ZW et al. Silencing of PPM1D inhibits cell proliferation and invasion through the p38 MAPK and p53 signaling pathway in papillary thyroid carcinoma. Oncol Rep 43:783-794 (2020). PubMed: 31922231
- Liu J et al. Characterization of BRCA1-deficient premalignant tissues and cancers identifies Plekha5 as a tumor metastasis suppressor. Nat Commun 11:4875 (2020). PubMed: 32978388