重组Anti-PPAR gamma抗体[EPR18516] (ab178860)
![Western blot - Anti-PPAR gamma antibody [EPR18516] (ab178860)](https://www.abcam.cn/ps/products/178/ab178860/Images/ab178860-273961-anti-ppar-gamma-antibody-epr18516-western-blot.jpg "Western blot - Anti-PPAR gamma antibody [EPR18516] (ab178860)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18516] to PPAR gamma
- Suitable for: Flow Cyt (Intra), WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-PPAR gamma抗体[EPR18516]
参阅全部 PPAR gamma 一抗 -
描述
兔单克隆抗体[EPR18516] to PPAR gamma -
宿主
Rabbit -
特异性
Expression levels of PPARy protein vary with sample type. Induction may be required if endogenous expression is low. -
经测试应用
适用于: Flow Cyt (Intra), WB, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Human adipose tissue, ovary and placenta lysates; K562 and HeLa whole cell lysates. ICC/IF: HeLa and K562 cells. Flow Cyt (intra): HeLa and K562 cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR18516 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Assay kits
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab178860于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
1/1500.
|
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| WB |
1/1000. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).
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| ICC/IF |
1/250.
|
| 说明 |
|---|
|
Flow Cyt (Intra)
1/1500. |
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WB
1/1000. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa). |
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ICC/IF
1/250. |
靶标
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功能
Receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once activated by a ligand, the receptor binds to a promoter element in the gene for acyl-CoA oxidase and activates its transcription. It therefore controls the peroxisomal beta-oxidation pathway of fatty acids. Key regulator of adipocyte differentiation and glucose homeostasis. -
组织特异性
Highest expression in adipose tissue. Lower in skeletal muscle, spleen, heart and liver. Also detectable in placenta, lung and ovary. -
疾病相关
Note=Defects in PPARG can lead to type 2 insulin-resistant diabetes and hyptertension. PPARG mutations may be associated with colon cancer.
Defects in PPARG may be associated with susceptibility to obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat.
Defects in PPARG are the cause of familial partial lipodystrophy type 3 (FPLD3) [MIM:604367]. Familial partial lipodystrophies (FPLD) are a heterogeneous group of genetic disorders characterized by marked loss of subcutaneous (sc) fat from the extremities. Affected individuals show an increased preponderance of insulin resistance, diabetes mellitus and dyslipidemia.
Genetic variations in PPARG can be associated with susceptibility to glioma type 1 (GLM1) [MIM:137800]. Gliomas are central nervous system neoplasms derived from glial cells and comprise astrocytomas, glioblastoma multiforme, oligodendrogliomas, and ependymomas. Note=Polymorphic PPARG alleles have been found to be significantly over-represented among a cohort of American patients with sporadic glioblastoma multiforme suggesting a possible contribution to disease susceptibility. -
序列相似性
Belongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 5468 Human
- Omim: 601487 Human
- SwissProt: P37231 Human
- Unigene: 162646 Human
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别名
- CIMT1 antibody
- GLM1 antibody
- NR1C3 antibody
see all
图片
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Western blot - Anti-PPAR gamma antibody [EPR18516] (ab178860)All lanes : Anti-PPAR gamma antibody [EPR18516] (ab178860) at 1/1000 dilution
Lane 1 : Human adipose tissue lysate
Lane 2 : Human ovary lysate
Lane 3 : Human placenta lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/20000 dilution
Predicted band size: 58 kDa
Observed band size: 58 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
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![Immunocytochemistry/ Immunofluorescence - Anti-PPAR gamma antibody [EPR18516] (ab178860)](https://www.abcam.cn/ps/products/178/ab178860/Images/ab178860-273958-anti-ppar-gamma-antibody-epr18516-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-PPAR gamma antibody [EPR18516] (ab178860)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling PPAR gamma with ab178860 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear and weak cytoplasmic staining on HeLa cell line.
The nuclear counter stain is DAPI (blue). Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.
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![Flow Cytometry (Intracellular) - Anti-PPAR gamma antibody [EPR18516] (ab178860)](https://www.abcam.cn/ps/products/178/ab178860/Images/ab178860-273956-anti-ppar-gamma-antibody-epr18516-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-PPAR gamma antibody [EPR18516] (ab178860)Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling PPAR gamma with ab178860 at 1/1500 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.
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![Immunocytochemistry/ Immunofluorescence - Anti-PPAR gamma antibody [EPR18516] (ab178860)](https://www.abcam.cn/ps/products/178/ab178860/Images/ab178860-273957-anti-ppar-gamma-antibody-epr18516-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-PPAR gamma antibody [EPR18516] (ab178860)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized K562 (Human chronic myelogenous leukemia cell line from bone marrow) cells labeling PPAR gamma with ab178860 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear and weak cytoplasmic staining on K562 cell line.
The nuclear counter stain is DAPI (blue). Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.
-
![Western blot - Anti-PPAR gamma antibody [EPR18516] (ab178860)](https://www.abcam.cn/ps/products/178/ab178860/Images/ab178860-273959-anti-ppar-gamma-antibody-epr18516-western-blot.jpg)
Western blot - Anti-PPAR gamma antibody [EPR18516] (ab178860)All lanes : Anti-PPAR gamma antibody [EPR18516] (ab178860) at 1/1000 dilution
Lane 1 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 58 kDa
Observed band size: 58 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lane 1: 10 seconds; Lane 2: 3 seconds.
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![Flow Cytometry (Intracellular) - Anti-PPAR gamma antibody [EPR18516] (ab178860)](https://www.abcam.cn/ps/products/178/ab178860/Images/ab178860-273955-anti-ppar-gamma-antibody-epr18516-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-PPAR gamma antibody [EPR18516] (ab178860)Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed K562 (Human chronic myelogenous leukemia cell line from bone marrow) cells labeling PPAR gamma with ab178860 at 1/1500 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.
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![Anti-PPAR gamma antibody [EPR18516] (ab178860)](https://www.abcam.cn/ps/products/178/ab178860/Images/ab178860-7-benefits-of-recombinant-antibodies.png)
Anti-PPAR gamma antibody [EPR18516] (ab178860)
实验方案
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (40)
ab178860 被引用在 40 文献中.
- Li X et al. Inhibition of miR-96-5p alleviates intervertebral disc degeneration by regulating the peroxisome proliferator-activated receptor γ/nuclear factor-kappaB pathway. J Orthop Surg Res 18:916 (2023). PubMed: 38041147
- Zhao X et al. Degenerated nucleus pulposus cells derived exosome carrying miR-27a-3p aggravates intervertebral disc degeneration by inducing M1 polarization of macrophages. J Nanobiotechnology 21:317 (2023). PubMed: 37667246
- Yang C et al. Rumen microbiota-host transcriptome interaction mediates the protective effects of trans-10, cis-12 CLA on facilitating weaning transition of lambs. Anim Nutr 12:345-359 (2023). PubMed: 36788929
- Lu Y et al. Icariin has the potential to induce the differentiation of bone marrow mesenchymal stem cells into brown fat cells via PDE5A inhibition. Heliyon 9:e22487 (2023). PubMed: 38094047
- Su Y et al. Wogonin regulates colonocyte metabolism via PPARγ to inhibit Enterobacteriaceae against dextran sulfate sodium-induced colitis in mice. Phytother Res 37:872-884 (2023). PubMed: 36451541