Anti-PPAR gamma 抗体

• WB

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Western blot - Anti-PPAR gamma antibody (AB209350)

All lanes:

Western blot - Anti-PPAR gamma antibody (ab209350)

Lane 1:

NIH/3T3 cell lysate

Lane 2:

HeLa cell lysate

Lane 3:

K562 cell lysate

Lane 4:

PC-12 cell lysate

Lane 5:

U-87 MG cell lysate

Predicted band size: 57 kDa

false

• ICC

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Immunocytochemistry - Anti-PPAR gamma antibody (AB209350)

Immunocytochemistry analysis of PPAR gamma (green) showing positive staining in the nucleus and cytoplasm of Hela cells (right) compared with a negative control in the absence of primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes, blocked with 3% BSA-PBS for 30 minutes at room temperature and probed with ab209350 in 3% BSA-PBS at a dilution of 1/200 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 488-conjugated goat-anti-rabbit IgG secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with DAPI for 5-10 minutes in the dark. Images were taken at a magnification of 60x.

• ICC

Supplier Data

Immunocytochemistry - Anti-PPAR gamma antibody (AB209350)

Immunocytochemistry analysis of 70% confluent log phase HeLa cells labeling PPAR Gamma with ab209350. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with ab209350 at 1/250 dilution in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor^® 488 conjugate at a dilution of 1/2000 for 45 minutes at room temperature (Panel a : green). Nuclei (Panel b : blue) were stained with SlowFade^® Gold Antifade Mountant with DAPI. F-actin (Panel c : red) was stained with Rhodamine Phalloidin, 1/300. Panel d is a merged image showing Nuclear localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.

• ICC

Supplier Data

Immunocytochemistry - Anti-PPAR gamma antibody (AB209350)

Immunocytochemistry analysis of 3T3-L1 differentiated day 7 cells labeling PPAR gamma-2 (red) with ab209350. The cells were fixed with 4% paraformaldehyde in PBS for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 15 minutes, and blocked with 3% BSA for 30 minutes at room temperature. Cells were stained with ab209350 at a dilution of 1/200 in blocking buffer for 1 hour at room temperature, and then incubated with a Goat anti-Rabbit IgG (H+L) Secondary Antibody, Dylight 680 at a dilution of 1/1000 for at least 30 minutes at room temperature in the dark (red). Lipids (green) were stained with HCS LipidTOX neutral lipid stain at a dilution of 1/200 for at least 30 minutes at room temperature in the dark. Nuclei (blue) were stained with Hoechst 33342. Images were taken on a EVOS FL Auto Imaging System at 20X magnification.

• ICC

Supplier Data

Immunocytochemistry - Anti-PPAR gamma antibody (AB209350)

Immunocytochemical analysis of PPAR gamma using ab209350 at the dilution 1/200.The image at the top shows 3T3-L1 cells differntiated (for 7 days) where PPAR gamma is shown in green. The image below shows 3T3-L1 undifferentiated cells where no PPAR gamma is detected.

• ICC

Supplier Data

Immunocytochemistry - Anti-PPAR gamma antibody (AB209350)

Immunocytochemistry analysis of PPAR gamma (green) showing positive staining in the nucleus and cytoplasm of NIH-3T3 cells (right) compared with a negative control in the absence of primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes, blocked with 3% BSA-PBS for 30 minutes at room temperature and probed with ab209350 in 3% BSA-PBS at a dilution of 1/200 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 488-conjugated goat-anti-rabbit IgG secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with DAPI for 5-10 minutes in the dark. Images were taken at a magnification of 60x.

• ICC

Supplier Data

Immunocytochemistry - Anti-PPAR gamma antibody (AB209350)

Immunocytochemical analysis of PPAR gamma using ab209350 at the dilution 1/200. 3T3-L1 cells providing positive signal have been differentiated for 7 days.

PPAR gamma is shown in red, lipid droplets (that indicates the proper differentiation of the cells) are shown in green.

• WB

Supplier Data

Western blot - Anti-PPAR gamma antibody (AB209350)

Specific band detected in 3T3-L1 day 7 differentiated lysate with no reactivity in 3T3 lysate.

All lanes:

Western blot - Anti-PPAR gamma antibody (ab209350) at 1/500 dilution

Lane 1:

3T3 at 20 µg

Lane 2:

3T3-L1 differentiated day 7 at 20 µg

Predicted band size: 57 kDa

false