Anti-PPAR gamma 2 + PPAR gamma抗体[A3409A] (ab41928)
Key features and details
- Mouse monoclonal [A3409A] to PPAR gamma 2 + PPAR gamma
- Suitable for: IHC-P, WB, IP
- Reacts with: Mouse, Rat, Human
- Isotype: IgG2a
概述
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产品名称
Anti-PPAR gamma 2 + PPAR gamma抗体[A3409A] -
描述
小鼠单克隆抗体[A3409A] to PPAR gamma 2 + PPAR gamma -
宿主
Mouse -
经测试应用
适用于: IHC-P, WB, IPmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Recombinant fragment corresponding to Human PPAR gamma aa 1-150. Baculovirus-expressed
Database link: P37231 -
阳性对照
- IHC: Rat adipose cells and rat placenta. WB: Nuclear lysate of 3T3l-1 cells induced for adipocyte differentiation
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常规说明
This product was changed from ascites to tissue culture supernatant on 3rd April 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7
Preservative: 0.1% Sodium azide
Physiological saline. -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
A3409A -
同种型
IgG2a -
研究领域
相关产品
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Assay kits
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ChIP Related Products
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab41928于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P |
Use at an assay dependent concentration.
Perform antigen retrieval by autoclave to preserve reproducibility. |
|
WB | (2) |
Use at an assay dependent concentration. Predicted molecular weight: 58 kDa.
A nuclear extraction is recommended. |
IP |
Use at an assay dependent concentration.
|
说明 |
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IHC-P
Use at an assay dependent concentration. Perform antigen retrieval by autoclave to preserve reproducibility. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 58 kDa. A nuclear extraction is recommended. |
IP
Use at an assay dependent concentration. |
靶标
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细胞定位
PPAR gamma 2: Nucleus. Cytoplasm. Redistributed from the nucleus to the cytosol through a MAP2K1/MEK1-dependent manner. PPAR gamma: Nucleus. -
数据库链接
- Entrez Gene: 5468 Human
- Entrez Gene: 19016 Mouse
- Entrez Gene: 25664 Rat
- Omim: 601487 Human
- SwissProt: P37231 Human
- SwissProt: P37238 Mouse
- SwissProt: O88275 Rat
- Unigene: 162646 Human
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPAR gamma 1+2 antibody [A3409A] (ab41928)
ab41928 staining PPAR gamma 1+2 in rat adiposoe cells (10 ug/mL) by immunohistochemistry, formalin-fixed paraffin embedded sections.
This image was generated using the ascites version of the product.
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All lanes : Anti-PPAR gamma 2 + PPAR gamma antibody [A3409A] (ab41928) at 1 µg/ml
Lane 1 : Nuclear lysate of non induced
3T3l-1 cells
Lane 2 : Nuclear lysate of 3T3l-1 cells induced for adipocyte differentiation
Lysates/proteins at 20 µg per lane.
Predicted band size: 58 kDaTwo bands are visible: PPAR gamma 1 and PPAR gamma 2.
This image was generated using the ascites version of the product.
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Lane 1: FLAG-M2
Lane 2: ab41928
Lane 3: ab41928
Lane 4: Control IgGImmunoprecipitation analysis using Fluorescence labeled PPAR gamma generated in vitro translation. Anti-PPAR gamma antibody (ab41928) is diluted with translation production and mixed with protein G Sepharose beads. It is then rotated at 4°C for 1 hour and precipitates are eluted with addition of SDS loading buffer. Samples are incubated for 10minutes at 70°C and analyzed with Fluorescence imager.
This image was generated using the ascites version of the product.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPAR gamma 1+2 antibody [A3409A] (ab41928)
ab41928 staining PPAR gamma 1+2 in rat plancenta (10 ug/mL) by immunohistochemistry, formalin-fixed paraffin embedded sections.
This image was generated using the ascites version of the product.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (53)
ab41928 被引用在 53 文献中.
- Zhang C et al. PM2.5 induced liver lipid metabolic disorders in C57BL/6J mice. Front Endocrinol (Lausanne) 14:1212291 (2023). PubMed: 37780625
- Lin B et al. Smurf2-induced degradation of SMAD2 causes inhibition of hair follicle stem cell differentiation. Cell Death Discov 8:160 (2022). PubMed: 35379779
- Wang H et al. GDF11 inhibits abnormal adipogenesis of condylar chondrocytes in temporomandibular joint osteoarthritis. Bone Joint Res 11:453-464 (2022). PubMed: 35787089
- Zhang T et al. Atorvastatin Exerts Protective Effect on Cardiopulmonary Bypass Induced Renal Injury in Rats via PPAR-γ. Heart Surg Forum 25:E124-E131 (2022). PubMed: 35238313
- Ma J et al. Role of HSP90α in osteoclast formation and osteoporosis development. Exp Ther Med 23:273 (2022). PubMed: 35251339