Anti-PPAR delta抗体(ab23673)
Key features and details
- Rabbit polyclonal to PPAR delta
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-PPAR delta抗体
参阅全部 PPAR delta 一抗 -
描述
兔多克隆抗体to PPAR delta -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WBmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rabbit, Chicken
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免疫原
Synthetic peptide corresponding to Human PPAR delta aa 1-100 (N terminal).
Database link: Q03181 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Assay kits
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab23673于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | (1) |
Use a concentration of 4 µg/ml.
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| WB | (6) |
Use a concentration of 2 µg/ml. Predicted molecular weight: 50 kDa. Nuclear extraction may be required in some samples. Detects a band of approximately 50 kDa in human samples, and an additional smaller size of PPAR delta is detected in some murine samples at approximately 40 kDa.
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| 说明 |
|---|
|
ICC/IF
Use a concentration of 4 µg/ml. |
|
WB
Use a concentration of 2 µg/ml. Predicted molecular weight: 50 kDa. Nuclear extraction may be required in some samples. Detects a band of approximately 50 kDa in human samples, and an additional smaller size of PPAR delta is detected in some murine samples at approximately 40 kDa. |
靶标
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功能
Ligand-activated transcription factor. Receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Has a preference for poly-unsaturated fatty acids, such as gamma-linoleic acid and eicosapentanoic acid. Once activated by a ligand, the receptor binds to promoter elements of target genes. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the acyl-CoA oxidase gene. Decreases expression of NPC1L1 once activated by a ligand. -
组织特异性
Ubiquitous with maximal levels in placenta and skeletal muscle. -
序列相似性
Belongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 395479 Chicken
- Entrez Gene: 5467 Human
- Entrez Gene: 19015 Mouse
- Omim: 600409 Human
- SwissProt: Q03181 Human
- SwissProt: P35396 Mouse
- Unigene: 696032 Human
- Unigene: 328914 Mouse
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别名
- FAAR antibody
- MGC3931 antibody
- NR1C2 antibody
see all
图片
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Western blot - Anti-PPAR delta antibody (ab23673)This image is courtesy of an anonymous AbreviewAnti-PPAR delta antibody (ab23673) at 1/1000 dilution + Rat brain tissue lysate at 100 µg
Secondary
HRP-conjugate Goat anti-rabbit IgG polyclonal at 1/2000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 54 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
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Immunocytochemistry/ Immunofluorescence - Anti-PPAR delta antibody (ab23673)Immunofluorescent staining of HT-29 cells (ab3952) (nuclear staining) using ab23673.
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Western blot - Anti-PPAR delta antibody (ab23673)All lanes : Anti-PPAR delta antibody (ab23673)
Lane 1 : Human cerebral cortex (30ug)
Lane 2 : Murine liver (30ug)
Predicted band size: 50 kDa
ab23673 recognises PPAR delta at 50kDa from human samples. An additional smaller size of PPAR delta (~40kDa) is also detected in certain murine tissues.
数据表及文件
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SDS download
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Datasheet download
文献 (47)
ab23673 被引用在 47 文献中.
- He H et al. Smad3 Mediates Diabetic Dyslipidemia and Fatty Liver in db/db Mice by Targeting PPARδ. Int J Mol Sci 24:N/A (2023). PubMed: 37511155
- Jia L et al. Low-dose adropin stimulates inflammasome activation of macrophage via mitochondrial ROS involved in colorectal cancer progression. BMC Cancer 23:1042 (2023). PubMed: 37904094
- Valdivia AO & Bhattacharya SK Lyso-Lipid-Induced Oligodendrocyte Maturation Underlies Restoration of Optic Nerve Function. eNeuro 9:N/A (2022). PubMed: 35027445
- Joseph LC et al. Combined metabolomic and transcriptomic profiling approaches reveal the cardiac response to high-fat diet. iScience 25:104184 (2022). PubMed: 35494220
- Brailey PM et al. CD1d-dependent rewiring of lipid metabolism in macrophages regulates innate immune responses. Nat Commun 13:6723 (2022). PubMed: 36344546