Anti-PPAR alpha (phospho S12)抗体(ab3484)
Key features and details
- Rabbit polyclonal to PPAR alpha (phospho S12)
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-PPAR alpha (phospho S12)抗体
参阅全部 PPAR alpha 一抗 -
描述
兔多克隆抗体to PPAR alpha (phospho S12) -
宿主
Rabbit -
特异性
The antibody is expected to bind both phospho and non phospho forms. -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Guinea pig, Dog
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免疫原
Synthetic peptide corresponding to Mouse PPAR alpha aa 1-100 (phospho S12).
Database link: P23204 -
阳性对照
- WB: human U-87, MCF7, MDA-MB-231, C2C12, HepG2, and mouse NIH-3T3 ICC/IF: C2C12, 3T3-L1, U-87 MG cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Nucleotide metabolism
- Molecular processes
- Mitochondrial transcription
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
靶标
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功能
Ligand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC). Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety (By similarity). Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2. -
组织特异性
Skeletal muscle, liver, heart and kidney. -
序列相似性
Belongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 403654 Dog
- Entrez Gene: 100135604 Guinea pig
- Entrez Gene: 5465 Human
- Entrez Gene: 19013 Mouse
- Omim: 170998 Human
- SwissProt: Q95N78 Dog
- SwissProt: O35507 Guinea pig
- SwissProt: Q07869 Human
see all -
别名
- hPPAR antibody
- MGC2237 antibody
- MGC2452 antibody
see all
图片
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Western blot - Anti-PPAR alpha (phospho S12) antibody (ab3484)All lanes : Anti-PPAR alpha (phospho S12) antibody (ab3484) at 1/200 dilution
Lane 1 : C2C12 cell lysate
Lane 2 : NIH-3T3 cell lysate
Lane 3 : 3T3-L1 cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 52 kDa
Observed band size: 52 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-PPAR alpha (phospho S12) antibody (ab3484)Immunofluorescent analysis of Phospho-PPAR alpha pSer12 (green) showing staining in the nucleus of C2C12 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Phospho-PPAR alpha pSer12 polyclonal antibody (ab3484) in 3% BSA-PBS at a dilution of 1:200 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
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Immunocytochemistry/ Immunofluorescence - Anti-PPAR alpha (phospho S12) antibody (ab3484)Immunofluorescent analysis of Phospho-PPAR alpha pSer12 (green) showing staining in the nucleus of 3T3-L1 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Phospho-PPAR alpha pSer12 polyclonal antibody (ab3484) in 3% BSA-PBS at a dilution of 1:200 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
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Western blot - Anti-PPAR alpha (phospho S12) antibody (ab3484)All lanes : Anti-PPAR alpha (phospho S12) antibody (ab3484) at 1/1000 dilution
Lane 1 : U-87 MG with Skimmed milk
Lane 2 : MCF7 with Skimmed milk
Lane 3 : MDA-MB-231 with Skimmed milk
Lane 4 : C2C12 with Skimmed milk
Lane 5 : Hep G2 with Skimmed milk
Lane 6 : NIH/3T3 with Skimmed milk
Lysates/proteins at 20 µg per lane.
Blocking peptides at 5 % per lane.
Secondary
All lanes : Goat anti-rabbit IgG (H+L) at 1/2500 dilution
Predicted band size: 52 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-PPAR alpha (phospho S12) antibody (ab3484)Immunofluorescent analysis of Phospho-PPAR alpha pSer12 (green) showing staining in the nucleus of U-87 MG cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Phospho-PPAR alpha pSer12 polyclonal antibody (ab3484) in 3% BSA-PBS at a dilution of 1:200 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
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Immunocytochemistry/ Immunofluorescence - Anti-PPAR alpha (phospho S12) antibody (ab3484)This image is courtesy of an anonymous Abreviewab3484 staining PPAR alpha (phospho S12) in Mouse neuronal cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 10% serum for 20 minutes at 25°C. Samples were incubated with primary antibody (1/100 in PBS) for 18 hours at 4°C. A Cy2®-conjugated Donkey anti-rabbit IgG polyclonal (1/100) was used as the secondary antibody.
实验方案
数据表及文件
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Datasheet download
文献 (34)
ab3484 被引用在 34 文献中.
- Güven B et al. Obesity Is a Major Determinant of Impaired Cardiac Energy Metabolism in Heart Failure with Preserved Ejection Fraction. J Pharmacol Exp Ther 388:145-155 (2024). PubMed: 37977817
- Azimzadeh O et al. Late Effects of Chronic Low Dose Rate Total Body Irradiation on the Heart Proteome of ApoE-/- Mice Resemble Premature Cardiac Ageing. Cancers (Basel) 15:N/A (2023). PubMed: 37444528
- Mi Y et al. Loss of fatty acid degradation by astrocytic mitochondria triggers neuroinflammation and neurodegeneration. Nat Metab 5:445-465 (2023). PubMed: 36959514
- Xu J et al. Replacement of Dietary Fishmeal with Clostridium autoethanogenum Protein on Lipidomics and Lipid Metabolism in Muscle of Pearl Gentian Grouper. Aquac Nutr 2023:6723677 (2023). PubMed: 37424881
- Zhu X et al. Effects of swimming before and during pregnancy on placental angiogenesis and perinatal outcome in high-fat diet-fed mice. PeerJ 11:e14562 (2023). PubMed: 36846460
