AB177935

重组Anti-PPA2抗体[EPR13087(B)]

Anti-PPA2 antibody [EPR13087(B)]

RabMAb
Recombinant
了解详情

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(4 Publications)

Rabbit Recombinant Monoclonal PPA2 antibody. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 4 publications.

查看别名

HSPC124, PPA2, Pyrophosphatase SID6-306, Pyrophosphate phospho-hydrolase 2, PPase 2

3 Images

Flow Cytometry (Intracellular) - Anti-PPA2 antibody [EPR13087(B)] (AB177935)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-PPA2 antibody [EPR13087(B)] (AB177935)

Intracellular flow cytometric analysis of permeabilized 293 cellslabeling PPA2 with ab177935 at 1/10 dilution (red) compared toa rabbit IgG negative control (green).

Supplier Data

Immunoprecipitation - Anti-PPA2 antibody [EPR13087(B)] (AB177935)

Western blot analysis on immunoprecipitation pellet from U87-MG cell lysate using ab177935.

All lanes:

Immunoprecipitation - Anti-PPA2 antibody [EPR13087(B)] (ab177935)

Predicted band size: 38 kDa

false

Supplier Data

Western blot - Anti-PPA2 antibody [EPR13087(B)] (AB177935)

All lanes:

Western blot - Anti-PPA2 antibody [EPR13087(B)] (ab177935) at 1/1000 dilution

Lane 1:

Human fetal liver tissue lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

Human fetal kidney tissue lysate at 10 µg

Lane 4:

U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cell lysate at 10 µg

Lane 5:

293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

Predicted band size: 38 kDa

false

不同偶联物与剂型 (1)

Carrier free

Anti-PPA2 antibody [EPR13087(B)] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR13087(B)

亚型

IgG

不含载体蛋白

反应种属

Human

应用

IP, Flow Cyt (Intra), WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." } } }

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产品详情

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯度

Tissue culture supernatant

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Hydrolyzes inorganic pyrophosphate (PubMed : 27523597). This activity is essential for correct regulation of mitochondrial membrane potential, and mitochondrial organization and function (PubMed : 27523598).

See full target information PPA2

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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Metabolites 13: PubMed37999237

2023

PPA1 Deficiency Causes a Deranged Galactose Metabolism Recognizable in Neonatal Screening.

Applications

Unspecified application

Species

Unspecified reactive species

Melanie T Achleitner,Judith J M Jans,Laura Ebner,Johannes Spenger,Vassiliki Konstantopoulou,René G Feichtinger,Karin Brugger,Doris Mayr,Ron A Wevers,Christian Thiel,Saskia B Wortmann,Johannes A Mayr

Genetics in medicine : official journal of the American College of Medical Genetics 23:2415-2425 PubMed34400813

2021

PPA2-associated sudden cardiac death: extending the clinical and allelic spectrum in 20 new families.

Applications

Unspecified application

Species

Unspecified reactive species

Anne Guimier,Melanie T Achleitner,Anne Moreau de Bellaing,Matthew Edwards,Loïc de Pontual,Kirti Mittal,Kyla E Dunn,Megan E Grove,Carolyn J Tysoe,Clémantine Dimartino,Jessie Cameron,Anil Kanthi,Anju Shukla,Florence van den Broek,Diptendu Chatterjee,Charlotte L Alston,Charlotte V Knowles,Laura Brett,Jan A Till,Tessa Homfray,Paul French,Georgia Spentzou,Noha A Elserafy,Kate S Lichkus,Bindu P Sankaran,Hannah L Kennedy,Peter M George,Alexa Kidd,Saskia B Wortmann,Dianna G Fisk,Tamara T Koopmann,Muhammad A Rafiq,Jason D Merker,Sumith Parikh,Priyanka Ahimaz,Robert G Weintraub,Alan S Ma,Christian Turner,Carolyn J Ellaway,Liza K Phillips,David R Thorburn,Wendy K Chung,Sajel L Kana,Ona M Faye-Petersen,Michelle L Thompson,Alexandre Janin,Karen McLeod,Ruth McGowan,Robert McFarland,Katta M Girisha,Deborah J Morris-Rosendahl,Anna C E Hurst,Claire L S Turner,Robert M Hamilton,Robert W Taylor,Fanny Bajolle,Christopher T Gordon,Jeanne Amiel,Johannes A Mayr,Kit Doudney

American journal of human genetics 99:674-682 PubMed27523597

2016

Sudden Cardiac Death Due to Deficiency of the Mitochondrial Inorganic Pyrophosphatase PPA2.

Applications

Unspecified application

Species

Unspecified reactive species

Hannah Kennedy,Tobias B Haack,Verity Hartill,Lavinija Mataković,E Regula Baumgartner,Howard Potter,Richard Mackay,Charlotte L Alston,Siobhan O'Sullivan,Robert McFarland,Grainne Connolly,Caroline Gannon,Richard King,Scott Mead,Ian Crozier,Wandy Chan,Chris M Florkowski,Martin Sage,Thomas Höfken,Bader Alhaddad,Laura S Kremer,Robert Kopajtich,René G Feichtinger,Wolfgang Sperl,Richard J Rodenburg,Jean Claude Minet,Angus Dobbie,Tim M Strom,Thomas Meitinger,Peter M George,Colin A Johnson,Robert W Taylor,Holger Prokisch,Kit Doudney,Johannes A Mayr

American journal of human genetics 99:666-673 PubMed27523598

2016

Biallelic PPA2 Mutations Cause Sudden Unexpected Cardiac Arrest in Infancy.

Applications

Unspecified application

Species

Unspecified reactive species

Anne Guimier,Christopher T Gordon,François Godard,Gianina Ravenscroft,Myriam Oufadem,Christelle Vasnier,Caroline Rambaud,Patrick Nitschke,Christine Bole-Feysot,Cécile Masson,Stéphane Dauger,Cheryl Longman,Nigel G Laing,Béatrice Kugener,Damien Bonnet,Patrice Bouvagnet,Sylvie Di Filippo,Vincent Probst,Richard Redon,Philippe Charron,Agnès Rötig,Stanislas Lyonnet,Alain Dautant,Loïc de Pontual,Jean-Paul di Rago,Agnès Delahodde,Jeanne Amiel

View all publications

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重组Anti-PPA2抗体[EPR13087(B)]