Name: Anti-PP2A alpha + beta antibody [Y119]
Brand: RabMAb
SKU: ab32141
Rating: 4 (5 reviews)

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Western blot - Anti-PP2A alpha + beta antibody [Y119] (ab32141)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [Y119] to PP2A alpha + beta
Suitable for: WB, IHC-P, Flow Cyt (Intra)
Reacts with: Human

Alexa Fluor® 488 Alexa Fluor® 647 Carrier Free

产品名称

Anti-PP2A alpha + beta抗体[Y119]
参阅全部 PP2A alpha + beta 一抗
描述

兔单克隆抗体[Y119] to PP2A alpha + beta
宿主

Rabbit
特异性

This antibody detects both alpha and beta isoforms of PP2A.
经测试应用

适用于: WB, IHC-P, Flow Cyt (Intra)more details
种属反应性

与反应: Human
预测可用于: Mouse, Rat
免疫原

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
表位

ab32141 reacts with an epitope located in the C terminal region of PP2A alpha and beta
阳性对照
- T47-D cell lysate and human breast carcinoma
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

形式

Liquid
存放说明

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
存储溶液

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
纯度

Protein A purified
克隆

单克隆
克隆编号

Y119
同种型

IgG
研究领域

Alternative Versions
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

The Abpromise guarantee

Abpromise™承诺保证使用ab32141于以下的经测试应用

“应用说明”部分下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用	Ab评论	说明
WB	(3)	1/5000 - 1/10000. Detects a band of approximately 36 kDa (predicted molecular weight: 35 kDa).
IHC-P		1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt (Intra)		1/20.

说明
WB 1/5000 - 1/10000. Detects a band of approximately 36 kDa (predicted molecular weight: 35 kDa).
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt (Intra) 1/20.

功能

PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGOL2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (By similarity). Can dephosphorylate SV40 large T antigen and p53/TP53. Dephosphorylates SV40 large T antigen, preferentially on serine residues 120, 123, 677, and perhaps 679. The C subunit was most active, followed by the AC form, which was more active than the ABC form, and activity of all three forms was strongly stimulated by manganese, and to a lesser extent by magnesium. Dephosphorylation by the AC form, but not C or ABC form is inhibited by small T antigen. Activates RAF1 by dephosphorylating it at 'Ser-259'.
序列相似性

Belongs to the PPP phosphatase family. PP-1 subfamily.
翻译后修饰

Reversibly methyl esterified on Leu-309. Carboxyl methylation may play a role in holoenzyme assembly, enhancing the affinity of the PP2A core enzyme for some, but not all, regulatory subunits. It varies during the cell cycle.
Phosphorylation of either threonine (by autophosphorylation-activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation.
细胞定位

Cytoplasm. Nucleus. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle pole. In prometaphase cells, but not in anaphase cells, localizes at centromeres. During mitosis, also found at spindle poles. Centromeric localization requires the presence of SGOL2.
Target information above from: UniProt accession P67775 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
数据库链接
- Entrez Gene: 5515 Human
- Entrez Gene: 5516 Human
- Entrez Gene: 19052 Mouse
- Entrez Gene: 19053 Mouse
- Entrez Gene: 24672 Rat
- Entrez Gene: 24673 Rat
- Omim: 176915 Human
- Omim: 176916 Human
- SwissProt: P62714 Human
- SwissProt: P67775 Human
- SwissProt: P62715 Mouse
- SwissProt: P63330 Mouse
- SwissProt: P62716 Rat
- SwissProt: P63331 Rat
- Unigene: 105818 Human
- Unigene: 260288 Mouse
- Unigene: 1271 Rat
see all
别名
- PP2A A antibody
- PP2A alpha antibody
- PP2A B antibody
- PP2A beta antibody
- PP2A-alpha antibody
- PP2A-beta antibody
- PP2AA_HUMAN antibody
- PP2Aalpha antibody
- PP2AB_HUMAN antibody
- PP2Abeta antibody
- PP2Ac antibody
- PP2CA antibody
- PP2Calpha antibody
- PP2CB antibody
- PPP2CA antibody
- PPP2CB antibody
- Protein phosphatase 2 catalytic subunit alpha isoform antibody
- Protein phosphatase 2 catalytic subunit beta isoform antibody
- Protein phosphatase type 2A catalytic subunit antibody
- Replication protein C antibody
- RP C antibody
- RP-C antibody
- RPC antibody
- Serine/threonine protein phosphatase 2A catalytic subunit alpha isoform antibody
- Serine/threonine protein phosphatase 2A catalytic subunit beta isoform antibody
- Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform antibody
see all

Western blot - Anti-PP2A alpha + beta antibody [Y119] (ab32141)

Anti-PP2A alpha + beta antibody [Y119] (ab32141) at 1/10000 dilution + T47-D cell lysate

Predicted band size: 35 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PP2A alpha + beta antibody [Y119] (ab32141)

ab32141,at a 1/250 dilution, staining human breast carcinoma by immunohistochemistry, Paraffin embedded tissue

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cytometry (Intracellular) - Anti-PP2A alpha + beta antibody [Y119] (ab32141)

Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labelling PP2A alpha + beta (red) with unpurified ab32141 at dilution of 1/20. Goat anti rabbit IgG (Alexa Fluorr^®488) at 1/2000 was used as the secondary antibody. Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control antibody was Rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary and secondary antibodies.
Anti-PP2A alpha + beta antibody [Y119] (ab32141)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

发表研究结果有使用 ab32141？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab32141 被引用在 34 文献中.

Yanagi T et al. Absence of ULK1 decreases AMPK activity in the kidney, leading to chronic kidney disease progression. Genes Cells 28:5-14 (2023). PubMed: 36318474
Kilpeläinen TP et al. Nonpeptidic Oxazole-Based Prolyl Oligopeptidase Ligands with Disease-Modifying Effects on α-Synuclein Mouse Models of Parkinson's Disease. J Med Chem 66:7475-7496 (2023). PubMed: 37248563
Liu Y et al. Diane-35 and Metformin Induce Autophagy and Apoptosis in Polycystic Ovary Syndrome Women with Early-Stage Endometrial Carcinoma. Genes (Basel) 13:N/A (2022). PubMed: 35052471
Gergs U et al. Protein Phosphatase 2A Improves Cardiac Functional Response to Ischemia and Sepsis. Int J Mol Sci 23:N/A (2022). PubMed: 35563079
Cui H et al. Prolyl oligopeptidase inhibition reduces alpha-synuclein aggregation in a cellular model of multiple system atrophy. J Cell Mol Med 25:9634-9646 (2021). PubMed: 34486218

View all Publications for this product

客户评价及客户问答

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1-6 of 6 Abreviews or Q&A

Application

Western blot

Sample

Mouse Cell lysate - whole cell (Mouse testis)

Loading amount

25 µg

Specification

Mouse testis

Gel Running Conditions

Reduced Denaturing (15 %)

Blocking step

Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

HERR DR. Vladimir Milenkovic

Verified customer

提交于 Jul 18 2011

Application

Immunoprecipitation

Sample

Mouse Cell lysate - whole cell (mouse testis)

Total protein in input

25 µg

Specification

mouse testis

Immuno-precipitation step

Protein G

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

HERR DR. Vladimir Milenkovic

Verified customer

提交于 Jul 14 2011

Application

Immunocytochemistry/ Immunofluorescence

Sample

Human Cell (HT1080 Cell line)

Specification

HT1080 Cell line

Fixative

Paraformaldehyde

Permeabilization

Yes - 0.1% Triton X-100

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jun 15 2010

Application

Western blot

Sample

Human Cell lysate - whole cell (HT1080 Cell line)

Loading amount

50000 cells

Specification

HT1080 Cell line

Gel Running Conditions

Reduced Denaturing (4-12%)

Blocking step

Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jun 04 2010

Application

Western blot

Sample

Human Cell lysate - whole cell (A549 lung carcinoma)

Loading amount

40 µg

Specification

A549 lung carcinoma

Gel Running Conditions

Reduced Denaturing (12%)

Blocking step

Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 4°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Apr 06 2009

Question

Product code: 32141

Inquiry: Hello, I'm working with filamentous fungi Neurospora crassa, I'd like to order antibody for protein phosphatase PP2A catalytic subunit. Visiting your website I saw a list of anti pp2a antibodies but my concern is that those antibodies will not link to Neurospora crassa protein PP2A as there are some differences in peptide sequence between human PP2A and Neurospora crassa PP2A catalytic subunit. I would appreciate your advice on this issue.

Abcam community

Verified customer

Asked on May 14 2012

Answer

Thank you for your inquiry.

We generally expect a cross reaction between species of an antibody when the homology is 85% and higher. We consider this then worth testing.

Unfortunately, the immunogenof ab32141 and Neurospora crassa only show 78% homology. Therefore I cannot recommend this antibody for your purposes.

I also check our other Anti-PP2A alpha and beta antibodies.

They all also show a homology that is too low to expect a cross reaction, apart fromab1309.

ab1309 shows a 84% homology with Neurospora crassa. This is borderline, but might be worth testing.

Therefore, I can offer a discount off a future purchase if you buy ab1309 now, test it in Neurosporacrassaand submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of: 1 free primary antibody or the value off your next order.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab1309 in Neurospora crassa. I will then send a discount code. This code must be issued before purchasing ab1309 so please wait for my reply before ordering.

2. Purchase ab1309 either by phone, fax, or online (www.abcam.com).

3. Test it in Neurospora crassa.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyproduct ordered and the discount code is valid for 4 months after issue.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab1309 turns out to be unsuitable for Neurospora crassa, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

Abcam Scientific Support

回复于 May 14 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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Key features and details

Related conjugates and formulations

概述

产品名称

描述

宿主

特异性

经测试应用

种属反应性

免疫原

表位

阳性对照

常规说明

性能

形式

存放说明

存储溶液

纯度

克隆

克隆编号

同种型

研究领域

相关产品

Alternative Versions

Compatible Secondaries

Isotype control

应用

The Abpromise guarantee

靶标

功能

序列相似性

翻译后修饰

细胞定位

数据库链接

别名

图片

实验方案

数据表及文件

SDS download

Datasheet download

文献 (34)

客户评价及客户问答

Western blot abreview for Anti-PP2A alpha and beta antibody [Y119]

Immunoprecipitation abreview for Anti-PP2A alpha and beta antibody [Y119]

Immunocytochemistry/ Immunofluorescence abreview for Anti-PP2A alpha and beta antibody [Y119]

Western blot abreview for Anti-PP2A alpha and beta antibody [Y119]

Western blot abreview for Anti-PP2A alpha and beta antibody [Y119]