Anti-POU4F3抗体(ab272908)
Key features and details
- Rabbit polyclonal to POU4F3
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-POU4F3抗体
参阅全部 POU4F3 一抗 -
描述
兔多克隆抗体to POU4F3 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Recombinant fragment within Human POU4F3. The exact sequence is proprietary. Center region.
Database link: Q15319 -
阳性对照
- WB: HeLa nuclear extract. IHC-P: Mouse eye tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab272908于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/3000.
|
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| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
WB
1/500 - 1/3000. |
|
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
May play a role in determining or maintaining the identities of a small subset of visual system neurons. -
组织特异性
Brain. Seems to be specific to the retina. -
疾病相关
Defects in POU4F3 are the cause of deafness autosomal dominant type 15 (DFNA15) [MIM:602459]. DFNA15 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information. -
序列相似性
Belongs to the POU transcription factor family. Class-4 subfamily.
Contains 1 homeobox DNA-binding domain.
Contains 1 POU-specific domain. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 5459 Human
- Entrez Gene: 18998 Mouse
- Omim: 602460 Human
- SwissProt: Q15319 Human
- SwissProt: Q63955 Mouse
- Unigene: 553499 Human
- Unigene: 221181 Mouse
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别名
- Brain specific homeobox/POU domain protein 3C antibody
- Brain-3C antibody
- Brain-specific homeobox/POU domain protein 3C antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POU4F3 antibody (ab272908)
Paraffin-embedded mouse eye tissue stained for POU4F3 using ab272908 at 1/500 dilution in immunohistochemical analysis. Antigen retrieval using citrate buffer, pH 6, 15 minutes.
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Western blot - Anti-POU4F3 antibody (ab272908)All lanes : Anti-POU4F3 antibody (ab272908) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HeLa nuclear extract lysate
Lysates/proteins at 30 µg per lane.12% SDS-PAGE.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab272908 尚未被引用在任何文献中。