Anti-POU3F3/Brain1抗体(ab106764)
Key features and details
- Goat polyclonal to POU3F3/Brain1
- Suitable for: WB, ICC, Flow Cyt
- Reacts with: Mouse
- Isotype: IgG
概述
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产品名称
Anti-POU3F3/Brain1抗体
参阅全部 POU3F3/Brain1 一抗 -
描述
山羊多克隆抗体to POU3F3/Brain1 -
宿主
Goat -
经测试应用
适用于: WB, ICC, Flow Cytmore details -
种属反应性
与反应: Mouse
预测可用于: Rat, Human
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免疫原
Synthetic peptide corresponding to Human POU3F3/Brain1 aa 89-101 (internal sequence).
Sequence:C-HMLSHAHQWVTAL
Database link: NP_006227.1 -
阳性对照
- WB: Mouse spinal cord and brain lysate; Flow cyt: Neuro-2a cells; ICC: Neuro-2a cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab106764于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 50 kDa).
1 hour primary incubation is recommended for this product. |
|
| ICC |
Use a concentration of 10 µg/ml.
|
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| Flow Cyt |
Use a concentration of 10 µg/ml.
|
| 说明 |
|---|
|
WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 50 kDa). 1 hour primary incubation is recommended for this product. |
|
ICC
Use a concentration of 10 µg/ml. |
|
Flow Cyt
Use a concentration of 10 µg/ml. |
靶标
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功能
Transcription factor that acts synergistically with SOX11 and SOX4. Plays a role in neuronal development. Is implicated in an enhancer activity at the embryonic met-mesencephalic junction; the enhancer element contains the octamer motif (5'-ATTTGCAT-3'). -
组织特异性
Brain. -
序列相似性
Belongs to the POU transcription factor family. Class-3 subfamily.
Contains 1 homeobox DNA-binding domain.
Contains 1 POU-specific domain. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 5455 Human
- Entrez Gene: 18993 Mouse
- Entrez Gene: 192109 Rat
- Omim: 602480 Human
- SwissProt: P20264 Human
- SwissProt: P31361 Mouse
- SwissProt: Q63262 Rat
- Unigene: 673855 Human
see all -
别名
- Brain 1 antibody
- Brain specific homeobox/POU domain protein 1 antibody
- Brain-1 antibody
see all
图片
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Flow Cytometry - Anti-POU3F3/Brain1 antibody (ab106764)
Flow cytometric analysis of paraformaldehyde fixed Neuro-2a cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Immunocytochemistry - Anti-POU3F3/Brain1 antibody (ab106764)Immunofluorescence analysis of paraformaldehyde fixed Neuro-2a cells, permeabilized with 0.15% Triton, labelling POU3F3/Brain1 with ab106764 for 1 hour at 10ug/mL followed by Alexa Fluor 488 secondary antibody at 2ug/mL, showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG at 10ug/mL followed by Alexa Fluor 488 secondary antibody at 2ug/mL.
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Western blot - Anti-POU3F3/Brain1 antibody (ab106764)All lanes : Anti-POU3F3/Brain1 antibody (ab106764) at 1 µg/ml
Lane 1 : Mouse spinal cord lysate
Lane 2 : Mouse brain lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 50 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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