AB106764

Anti-POU3F3/Brain1抗体

Name: POU3F3/Brain1抗体 (ab106764)| Abcam中文官网
Brand: Abcam Limited
SKU: AB106764
Price: 4606 CNY
Availability: InStock

Anti-POU3F3/Brain1 antibody

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(2 Publications)

Goat Polyclonal POU3F3/Brain1 antibody. Suitable for ICC, Flow Cyt, WB and reacts with Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human POU3F3 aa 50-150.

查看别名

BRN1, OTF8, POU3F3, Brain-specific homeobox/POU domain protein 1, Octamer-binding protein 8, Octamer-binding transcription factor 8, Brain-1, Brn-1, Oct-8, OTF-8

3 Images

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Immunocytochemistry - Anti-POU3F3/Brain1 antibody (AB106764)

Immunofluorescence analysis of paraformaldehyde fixed Neuro-2a cells, permeabilized with 0.15% Triton, labelling POU3F3/Brain1 with ab106764 for 1 hour at 10ug/mL followed by Alexa Fluor 488 secondary antibody at 2ug/mL, showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control : Unimmunized goat IgG at 10ug/mL followed by Alexa Fluor 488 secondary antibody at 2ug/mL.

Flow Cytometry - Anti-POU3F3/Brain1 antibody (AB106764)

Flow Cyt

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Flow Cytometry - Anti-POU3F3/Brain1 antibody (AB106764)

Flow cytometric analysis of paraformaldehyde fixed Neuro-2a cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control : Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

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Western blot - Anti-POU3F3/Brain1 antibody (AB106764)

All lanes:

Western blot - Anti-POU3F3/Brain1 antibody (ab106764) at 1 µg/mL

Lane 1:

Mouse spinal cord lysate at 35 µg

Lane 2:

Mouse brain lysate at 35 µg

Predicted band size: 50 kDa

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关键信息

宿主种属

Goat

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Mouse

应用

ICC, WB, Flow Cyt

applications

免疫原

Synthetic Peptide within Human POU3F3 aa 50-150. The exact immunogen used to generate this antibody is proprietary information.

P20264

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "10 µg/mL", "ICC-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "10 µg/mL", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-3 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product." }, "Rat": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

纯化说明

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

存储溶液

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Transcription factor that acts synergistically with SOX11 and SOX4. Plays a role in neuronal development (PubMed : 31303265). Is implicated in an enhancer activity at the embryonic met-mesencephalic junction; the enhancer element contains the octamer motif (5'-ATTTGCAT-3') (By similarity).

See full target information POU3F3

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Diseases (Basel, Switzerland) 11: PubMed37873783

2023

Systematic Evaluation of the Distribution of Immune Cells following Subcutaneous Administration of Haemophilus Influenzae Type B Vaccine to Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yao He,Yuxiu Zhao,Hongyang Liang,Xue Wang,Haoyue Lan,Dongyang Tian,Yan Li,Hui Wang

Pathology international 64:415-22 PubMed25243889

2014

Class III/IV POU transcription factors expressed in small cell lung cancer cells are involved in proneural/neuroendocrine differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Ishii,Hanako Sato,Takuya Yazawa,Yukiko Shishido-Hara,Chie Hiramatsu,Yukio Nakatani,Hiroshi Kamma

View all publications

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Anti-POU3F3/Brain1抗体

Anti-POU3F3/Brain1 antibody

Immunocytochemistry - Anti-POU3F3/Brain1 antibody (AB106764)

Flow Cytometry - Anti-POU3F3/Brain1 antibody (AB106764)

Western blot - Anti-POU3F3/Brain1 antibody (AB106764)