重组Anti-POT1抗体[S06-9I1] (ab317397)
![Western blot - Anti-POT1 Antibody [S06-9I1] (ab317397)](https://www.abcam.cn/ps/products/317/ab317397/Images/ab317397-536528-anti-pot1-antibody-s06-9i1-western-blot-k562-c6-3t3-human-rat-mouse.jpg "Western blot - Anti-POT1 Antibody [S06-9I1] (ab317397)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [S06-9I1] to POT1
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
概述
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产品名称
Anti-POT1抗体[S06-9I1]
参阅全部 POT1 一抗 -
描述
兔单克隆抗体[S06-9I1] to POT1 -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Human POT1.
Database link: Q9NUX5 -
阳性对照
- WB: K562, C6 and NIH/3T3 cell lysate.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.4
Preservative: 0.01% Sodium azide
Constituents: 55.08% Water, 40% Glycerol (glycerin, glycerine), 0.98% Tris glycine, 0.88% Sodium chloride, 0.05% BSA -
Concentration information loading... -
纯度
Affinity purified -
克隆
单克隆 -
克隆编号
S06-9I1 -
同种型
IgG -
研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab317397于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/5000. Predicted molecular weight: 71 kDa.
|
| 说明 |
|---|
|
WB
1/1000 - 1/5000. Predicted molecular weight: 71 kDa. |
靶标
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功能
Component of the telomerase ribonucleoprotein (RNP) complex that is essential for the replication of chromosome termini. Is a component of the double-stranded telomeric DNA-binding TRF1 complex which is involved in the regulation of telomere length by cis-inhibition of telomerase. Also acts as a single-stranded telomeric DNA-binding protein and thus may act as a downstream effector of the TRF1 complex and may transduce information about telomere maintenance and/or length to the telomere terminus. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded TTAGGG repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. Binds to two or more telomeric single-stranded 5'-TTAGGG-3' repeats (G-strand) and with high specificity to a minimal telomeric single-stranded 5'-TAGGGTTAG-3' sequence. Binds telomeric single-stranded sequences internally or at proximity of a 3'-end. Its activity is TERT dependent but it does not increase TERT activity by itself. In contrast, the ACD-POT1 heterodimer enhances telomer elongation by increasing telomerase processivity. -
组织特异性
Ubiquitous. -
序列相似性
Belongs to the telombin family. -
细胞定位
Nucleus. Chromosome > telomere. Colocalizes with telomeric DNA. - Information by UniProt
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数据库链接
- Entrez Gene: 25913 Human
- Entrez Gene: 101185 Mouse
- Entrez Gene: 500054 Rat
- Omim: 606478 Human
- SwissProt: Q9NUX5 Human
- SwissProt: Q91WC1 Mouse
- Unigene: 31968 Human
- Unigene: 274589 Mouse
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别名
- DKFZP586D211 antibody
- hPot 1 antibody
- hPot1 antibody
see all
图片
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Western blot - Anti-POT1 Antibody [S06-9I1] (ab317397)All lanes : Anti-POT1 Antibody [S06-9I1] (ab317397) at 1/1000 dilution
Lane 1 : K562 cell lysate
Lane 2 : C6 cell lysate
Lane 3 : NIH/3T3 cell lysate
Predicted band size: 71 kDa
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab317397 尚未被引用在任何文献中。