重组Anti-PON2抗体[EPR15295-58] (ab183718)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15295-58] to PON2
- Suitable for: WB, ICC/IF, IP
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-PON2抗体[EPR15295-58]
参阅全部 PON2 一抗 -
描述
兔单克隆抗体[EPR15295-58] to PON2 -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IF, IPmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- HeLa, U87-MG and HepG2 whole cell lysate. HeLa cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR15295-58 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab183718于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/1000 - 1/10000. Detects a band of approximately 39, 41 kDa (predicted molecular weight: 39 kDa).
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ICC/IF |
1/100.
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IP |
1/30 - 1/50.
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说明 |
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WB
1/1000 - 1/10000. Detects a band of approximately 39, 41 kDa (predicted molecular weight: 39 kDa). |
ICC/IF
1/100. |
IP
1/30 - 1/50. |
靶标
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功能
Capable of hydrolyzing lactones and a number of aromatic carboxylic acid esters. Has antioxidant activity. Is not associated with high density lipoprotein. Prevents LDL lipid peroxidation, reverses the oxidation of mildly oxidized LDL, and inhibits the ability of MM-LDL to induce monocyte chemotaxis. -
组织特异性
Widely expressed with highest expression in liver, lung, placenta, testis and heart. -
序列相似性
Belongs to the paraoxonase family. -
翻译后修饰
The signal sequence is not cleaved. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 5445 Human
- Entrez Gene: 330260 Mouse
- Entrez Gene: 296851 Rat
- Omim: 602447 Human
- SwissProt: Q15165 Human
- SwissProt: Q62086 Mouse
- SwissProt: Q6AXM8 Rat
- Unigene: 727553 Human
see all -
别名
- A esterase 2 antibody
- A-esterase 2 antibody
- Aromatic esterase 2 antibody
see all
图片
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All lanes : Anti-PON2 antibody [EPR15295-58] (ab183718) at 1/1000 dilution
Lane 1 : Mouse brain tissue lysate with NFDM/TBST
Lane 2 : Mouse heart tissue lysate with NFDM/TBST
Lane 3 : Mouse kidney tissue lysate with NFDM/TBST
Lane 4 : Mouse spleen tissue lysate with NFDM/TBST
Lane 5 : Rat brain tissue lysate with NFDM/TBST
Lane 6 : Rat heart tissue lysate with NFDM/TBST
Lane 7 : Rat kidney tissue lysate with NFDM/TBST
Lane 8 : Rat spleen tissue lysate with NFDM/TBST
Lane 9 : C6 (Rat glial tumor glial cell) whole cell lysate with NFDM/TBST
Lane 10 : Raw264.7 (Mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate with NFDM/TBST
Lane 11 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate with NFDM/TBST
Lane 12 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate with NFDM/TBST
Lysates/proteins at 20 µg per lane.
Blocking peptides at 5 % per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 39 kDa
Observed band size: 39 kDa
Exposure time: 30 seconds -
All lanes : Anti-PON2 antibody [EPR15295-58] (ab183718) at 1/1000 dilution
Lane 1 : HeLa whole cell lysate
Lane 2 : U87-MG whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 39 kDaBlocking/ Dilution buffer: 5% NFDM /TBST.
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Anti-PON2 antibody [EPR15295-58] (ab183718) at 1/5000 dilution + HepG2 whole cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 39 kDaBlocking/ Dilution buffer: 5% NFDM /TBST.
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Immunofluorescent analysis of acetone fixed HeLa cells labeling PON2 using ab183718 at a 1/100 dilution. A Goat anti rabbit IgG (Alexa Fluor®555) was used as the secondary at a 1/200 dilution.
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Lysate from HepG2 cells was immunoprecipitated with ab183718 at a 1/50 dilution. For the subsequent blot, ab183718 was used at a 1/1000 dilution with Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at a 1/1500 dilution for the secondary. Blocking/ Dilution buffer: 5% NFDM/TBST. Lane 2: TBS instead of HepG2 lysates.
实验方案
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (1)
ab183718 被引用在 1 文献中.
- Shin GC et al. Paraoxonase-2 contributes to promoting lipid metabolism and mitochondrial function via autophagy activation. Sci Rep 12:21483 (2022). PubMed: 36509805