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AB210605

重组Anti-POMC抗体[EPR17571]

Anti-POMC antibody [EPR17571]

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(12 Publications)

Rabbit Recombinant Monoclonal POMC antibody. Suitable for IP, WB, IHC-P and reacts with Rat, Mouse, Human, Synthetic peptide samples. Cited in 12 publications.

查看别名

Pro-opiomelanocortin, POMC, Corticotropin-lipotropin

13 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POMC antibody [EPR17571] (AB210605)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POMC antibody [EPR17571] (AB210605)

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue labeling POMC with ab210605 at 1/16000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Negative staining on human colon cancer.

Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POMC antibody [EPR17571] (AB210605)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POMC antibody [EPR17571] (AB210605)

Immunohistochemical analysis of paraffin-embedded human pituitary tissue labeling POMC with ab210605 at 1/16000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasmic staining on human pituitary is observed.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POMC antibody [EPR17571] (AB210605)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POMC antibody [EPR17571] (AB210605)

Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling POMC with ab210605 at 1/16000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Negative staining on human kidney.

Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POMC antibody [EPR17571] (AB210605)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POMC antibody [EPR17571] (AB210605)

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling POMC with ab210605 at 1/16000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Negative staining on mouse kidney.

Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POMC antibody [EPR17571] (AB210605)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POMC antibody [EPR17571] (AB210605)

Immunohistochemical analysis of paraffin-embedded rat pituitary tissue labeling POMC with ab210605 at 1/16000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasmic staining on rat pituitary is observed.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POMC antibody [EPR17571] (AB210605)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POMC antibody [EPR17571] (AB210605)

Immunohistochemical analysis of paraffin-embedded mouse pituitary tissue labeling POMC with ab210605 at 1/16000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasmic staining on mouse pituitary is observed.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POMC antibody [EPR17571] (AB210605)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POMC antibody [EPR17571] (AB210605)

Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling POMC with ab210605 at 1/16000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Negative staining on rat liver.

Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunoprecipitation - Anti-POMC antibody [EPR17571] (AB210605)
  • IP

Lab

Immunoprecipitation - Anti-POMC antibody [EPR17571] (AB210605)

ab210605 at 1/80 immunoprecipitating POMC in Rat pituitary whole cell lysate observed at 29 KDa (lanes 1 and 2).

Lane 1 (input) : Rat pituitary whole cell lysate 10μg

Lane 2 (+) : ab210605 + Rat pituitary whole cell lysate

Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab210605 in Rat pituitary whole cell lysate

For western blotting, ab210605 at 1/1000 dilution and VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

Blocking/Diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-POMC antibody [EPR17571] (ab210605)

Predicted band size: 29 kDa

false

Exposure time: 1s

Western blot - Anti-POMC antibody [EPR17571] (AB210605)
  • WB

Supplier Data

Western blot - Anti-POMC antibody [EPR17571] (AB210605)

Blocking/Dilution buffer : 5% NFDM/TBST.

Human ACTH recombinant peptide contains 3*aa138-176 with His-tag.

This product is capable of recognizing the ACTH peptide.

All lanes:

Western blot - Anti-POMC antibody [EPR17571] (ab210605) at 1/1000 dilution

All lanes:

human ACTH recombinant protein at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 29 kDa

Observed band size: 18 kDa

false

Exposure time: 3min

Western blot - Anti-POMC antibody [EPR17571] (AB210605)
  • WB

Supplier Data

Western blot - Anti-POMC antibody [EPR17571] (AB210605)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-POMC antibody [EPR17571] (ab210605) at 1/5000 dilution

All lanes:

mouse pituitary lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 29 kDa

Observed band size: 29 kDa

false

Exposure time: 3min

Western blot - Anti-POMC antibody [EPR17571] (AB210605)
  • WB

Supplier Data

Western blot - Anti-POMC antibody [EPR17571] (AB210605)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-POMC antibody [EPR17571] (ab210605) at 1/5000 dilution

All lanes:

rat pituitary lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 29 kDa

Observed band size: 29 kDa

false

Exposure time: 3min

Western blot - Anti-POMC antibody [EPR17571] (AB210605)
  • WB

Supplier Data

Western blot - Anti-POMC antibody [EPR17571] (AB210605)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-POMC antibody [EPR17571] (ab210605) at 1/1000 dilution

All lanes:

rat pituitary lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 29 kDa

Observed band size: 29 kDa

false

Exposure time: 1s

Western blot - Anti-POMC antibody [EPR17571] (AB210605)
  • WB

Supplier Data

Western blot - Anti-POMC antibody [EPR17571] (AB210605)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-POMC antibody [EPR17571] (ab210605) at 1/10000 dilution

Lane 1:

Untransfected HEK-293T (Human epithelial cell line from embryonic kidney) whole cell lysate at 10 µg

Lane 2:

HEK-293T (Human epithelial cell line from embryonic kidney) transfected with POMC whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 29 kDa

Observed band size: 55 kDa

false

Exposure time: 3s

不同偶联物与剂型 (1)

  • Carrier free

    Anti-POMC antibody [EPR17571] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR17571

亚型

IgG

不含载体蛋白

No

反应种属

Mouse, Rat, Human

应用

WB, IP, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/16000", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/16000", "IHCP-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/80", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/16000", "IHCP-species-notes": "<p></p>" }, "Synthetic peptide": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "1/80", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/16000", "IHCP-species-notes": "<p></p>" } } }
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产品详情

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
运输条件
Conditional Ambient
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Precursor protein of pituitary hormones that are involved in diverse physiological processes, including the regulation of energy balance, stress response, immune function and skin pigmentation.. Corticotropin. Functions as a ligand for the melanocortin receptors MC1R, MC2R, MC3R and MC5R (PubMed : 8396929, PubMed : 8463333, PubMed : 8636348). Activation of MC1R increases melanogenesis in melanocytes found in the skin and hair (PubMed : 9620771). Binding to MC2R stimulates the adrenal glands to secrete cortisol (PubMed : 8636348). Contributes to the regulation of energy homeostasis through activation of MC3R (Probable). Involved in the regulation exocrine gland function through MC5R activation (By similarity).. Melanocyte-stimulating hormone alpha. Serves as a ligand for the melanocortin receptors MC1R, MC3R, MC4R and MC5R (PubMed : 8392067, PubMed : 8396929, PubMed : 8463333). Activation of MC1R promotes melanogenesis in melanocytes of the skin and hair (PubMed : 10403794, PubMed : 9620771). Contributes to the regulation of energy homeostasis through activation of MC3R (Probable). Through MC4R activation, functions as an anorexigenic peptide (PubMed : 8392067). Promotes immunosuppression and involved in the regulation exocrine gland function through MC5R activation (By similarity).. Melanocyte-stimulating hormone beta. Functions as a ligand for the melanocortin receptors MC1R, MC3R, MC4R and MC5R (PubMed : 8396929, PubMed : 8463333). Activation of MC1R increases melanogenesis in melanocytes found in the skin and hair (Probable). Contributes to the regulation of energy homeostasis through activation of MC3R (Probable). Through MC4R activation, functions as an anorexigenic peptide (PubMed : 8392067). Involved in the regulation exocrine gland function through MC5R activation (By similarity).. Melanotropin gamma. Functions as a ligand for the melanocortin receptors MC1R, MC3R, MC4R and MC5R (PubMed : 8396929, PubMed : 8463333). Activation of MC1R increases melanogenesis in melanocytes found in the skin and hair (Probable). Contributes to the regulation of energy homeostasis through activation of MC3R (Probable). Binds to MC4R with low potency (PubMed : 8392067). Involved in the regulation exocrine gland function through MC5R activation (By similarity).. Beta-endorphin. Endogenous orexigenic opiate.. Met-enkephalin. Endogenous opiate.
See full target information POMC
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文献 (12)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in endocrinology 16:1585216 PubMed40416526

2025

Fatty acid metabolism after short-term fasting: POMC response and EPA signal maintain homeostasis in tilapia.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaozheng Yu,Tiansheng Zhu,Yang Yu,Ran Cai,Meiqing Li,Caiyun Sun,Wensheng Li

BMC biology 22:296 PubMed39710657

2024

Chronic sleep deprivation disturbs energy balance modulated by suprachiasmatic nucleus efferents in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Tingting Du,Shuailing Liu,Honghong Yu,Tian Hu,Lina Huang,Lanyue Gao,Lihong Jia,Jiajin Hu,Yang Yu,Qi Sun

Nature communications 14:5601 PubMed37699899

2023

Solar ultraviolet B radiation promotes α-MSH secretion to attenuate the function of ILC2s via the pituitary-lung axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yuying Huang,Lin Zhu,Shipeng Cheng,Ranran Dai,Chunrong Huang,Yanyan Song,Bo Peng,Xuezhen Li,Jing Wen,Yi Gong,Yunqian Hu,Ling Qian,Linyun Zhu,Fengying Zhang,Li Yu,Chunyan Yi,Wangpeng Gu,Zhiyang Ling,Liyan Ma,Wei Tang,Li Peng,Guochao Shi,Yaguang Zhang,Bing Sun

iScience 26:107525 PubMed37646018

2023

Novel human pluripotent stem cell-derived hypothalamus organoids demonstrate cellular diversity.

Applications

Unspecified application

Species

Unspecified reactive species

Lily Sarrafha,Drew R Neavin,Gustavo M Parfitt,Ilya A Kruglikov,Kristen Whitney,Ricardo Reyes,Elena Coccia,Tatyana Kareva,Camille Goldman,Regine Tipon,Gist Croft,John F Crary,Joseph E Powell,Joel Blanchard,Tim Ahfeldt

BMC endocrine disorders 21:185 PubMed34517852

2021

The genomic profiling and MAMLD1 expression in human and canines with Cushing's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Andrew Wang,Stewart G Neill,Scott Newman,Marianna A Tryfonidou,Adriana Ioachimescu,Michael R Rossi,Björn P Meij,Nelson M Oyesiku

Journal of neuroinflammation 18:159 PubMed34275474

2021

C1q/TNF-related protein 4 restores leptin sensitivity by downregulating NF-κB signaling and microglial activation.

Applications

Unspecified application

Species

Unspecified reactive species

Liu Ye,Gongwei Jia,Yuejie Li,Ying Wang,Hong Chen,Lehua Yu,Dandong Wu

Endocrine 72:340-348 PubMed33786714

2021

A potential impact of SARS-CoV-2 on pituitary glands and pituitary neuroendocrine tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Ting Gu,Fen Zhou,Wan Qun Xie,Shuo Wang,Hong Yao,Yan Ting Liu,Ling Gao,Zhe Bao Wu

Behavioural brain research 390:112660 PubMed32387350

2020

Maternal exercise during gestation and lactation decreases high-fat diet preference by altering central reward system gene expression in adult female offspring from high-fat fed dams.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Song,Jiaqi Cui,Ning Wang,Rui Wang,Jianqun Yan,Bo Sun

The Journal of clinical endocrinology and metabolism 105: PubMed32232325

2020

Germline CDKN1B Loss-of-Function Variants Cause Pediatric Cushing's Disease With or Without an MEN4 Phenotype.

Applications

Unspecified application

Species

Unspecified reactive species

Fanny Chasseloup,Nathan Pankratz,John Lane,Fabio R Faucz,Margaret F Keil,Prashant Chittiboina,Denise M Kay,Tara Hussein Tayeb,Constantine A Stratakis,James L Mills,Laura C Hernández-Ramírez

Obesity (Silver Spring, Md.) 28:122-131 PubMed31773909

2019

Liraglutide Lowers Body Weight Set Point in DIO Rats and its Relationship with Hypothalamic Microglia Activation.

Applications

Unspecified application

Species

Unspecified reactive species

Ting Liao,Shan-Lei Zhang,Xue Yuan,Wen-Qing Mo,Fang Wei,Sheng-Nan Zhao,Wei Yang,Hong Liu,Xi Rong
View all publications
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