重组Anti-Polyethylene glycol抗体[PEG-B-47b] - BSA and Azide free (ab271853)
![ELISA - Anti-Polyethylene glycol antibody [PEG-B-47b] - BSA and Azide free (ab271853)](https://www.abcam.cn/ps/products/271/ab271853/Images/ab271853-5-ab53449252954antipolyethyleneglycolantibodypegb47bbiotinelisa.jpg "ELISA - Anti-Polyethylene glycol antibody [PEG-B-47b] - BSA and Azide free (ab271853)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [PEG-B-47b] to Polyethylene glycol - BSA and Azide free
- Suitable for: Sandwich ELISA, WB, IHC-P, ELISA
Related conjugates and formulations
概述
-
产品名称
Anti-Polyethylene glycol抗体[PEG-B-47b] - BSA and Azide free
参阅全部 Polyethylene glycol 一抗 -
描述
兔单克隆抗体[PEG-B-47b] to Polyethylene glycol - BSA and Azide free -
宿主
Rabbit -
特异性
ab271853 does not cross react with Tween20.
-
经测试应用
适用于: Sandwich ELISA, WB, IHC-P, ELISAmore details -
免疫原
Chemical/ Small Molecule. This information is proprietary to Abcam and/or its suppliers.
-
常规说明
The smallest PEG molecule which our antibody can detect is PEG750. Based on customer feedback, the largest PEG molecule detected is PEG 40K. Additionally, ab271853 detects both linear and branched PEG based on our in-house ELISA testing.
Tween 20 usage: Tween 20 is a detergent that can interfere with ELISA and potentially other applications (often used in antibody dilution buffers, such as TBST) when using anti-PEG products. Our anti-mPEG antibody [PEG-B-47b] reacts to the methoxy group of Polyethylene glycol (PEG). There is no methoxy group in Tween 20, which should not interfere with the function of this antibody.
If you have any questions on our PEG products - please visit our Polyethylene glycol (PEG) FAQs page.
ab271853 is the unconjugated, carrier-free version of ab53449.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
解离常数（KD）
KD = 2.41 x 10 -9 M Learn more about KD -
存储溶液
pH: 7.2
Constituent: PBS -
无载体
是 -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
PEG-B-47b -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
-
Compatible Secondaries
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab271853于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
|
|
| WB |
Use at an assay dependent concentration.
|
|
| IHC-P |
1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
|
| ELISA |
Use a concentration of 0.5 µg/ml.
|
| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
|
WB
Use at an assay dependent concentration. |
|
IHC-P
1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
ELISA
Use a concentration of 0.5 µg/ml. |
靶标
-
别名
- PEG antibody
图片
-
ELISA - Anti-Polyethylene glycol antibody [PEG-B-47b] - BSA and Azide free (ab271853)
Direct ELISA antigen dose-response curve using purified ab53449. Antigen concentration of 0-500 ng/mL. A peroxidase-conjugated steptavidin (1/1000) was used.
Biotin conjugate (ab53449) available for this clone.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab53449). -
![Sandwich ELISA - Anti-Polyethylene glycol antibody [PEG-B-47b] - BSA and Azide free (ab271853)](https://www.abcam.cn/ps/products/271/ab271853/Images/ab271853-4-ab53449252952antipolyethyleneglycolantibodypegb47bbiotinsandwichelisa.jpg)
Sandwich ELISA - Anti-Polyethylene glycol antibody [PEG-B-47b] - BSA and Azide free (ab271853)Sandwich ELISA antigen dose-response curve using purified ab53449.
Capture antibody - Mouse anti-IFN alpha 2b (4000 ng/ml).
Antigens - PEG-IFN alpha 2b (0-1000 ng/ml).
Detection - ab53449 (500 ng) anti-Polyethylene glycol antibody [PEG-B-47b] (Biotin) followed by peroxidase-conjugated streptravidin (1/1000).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab53449). -
![ELISA - Anti-Polyethylene glycol antibody [PEG-B-47b] - BSA and Azide free (ab271853)](https://www.abcam.cn/ps/products/271/ab271853/Images/ab271853-1-ab5344966218antipolyethyleneglycolantibodypegb47bbiotinelisa.jpg)
ELISA - Anti-Polyethylene glycol antibody [PEG-B-47b] - BSA and Azide free (ab271853)ELISA assay using 5 µg/mL Anti-AFP RabMAb as the capture antibody and 0.5 µg/mL Anti-PEG-47-Biotin RabMAb (unpurified ab53449) as the detection antibody. HRP-avidin (1/3000) used for anti-PEG-47 detection. This ELISA assay could detect YCA1017 peptide-PEG antigen at a concentration as low as 0.9 ng/mL.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab53449). -
![ELISA - Anti-Polyethylene glycol antibody [PEG-B-47b] - BSA and Azide free (ab271853)](https://www.abcam.cn/ps/products/271/ab271853/Images/ab271853-2-ab5344966216antipolyethyleneglycolantibodypegb47bbiotinelisa.jpg)
ELISA - Anti-Polyethylene glycol antibody [PEG-B-47b] - BSA and Azide free (ab271853)Direct ELISA assay using 63 ng/mL of antigen (GCSF-PEG) to coat the plate. HRP-avidin (1/3000) was used for the detection of varying amounts of anti-PEG-47-Biotin (unpurified ab53449).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab53449). -
![Anti-Polyethylene glycol antibody [PEG-B-47b] - BSA and Azide free (ab271853)](https://www.abcam.cn/ps/products/271/ab271853/Images/ab271853-6-benefits-of-recombinant-antibodies.png)
Anti-Polyethylene glycol antibody [PEG-B-47b] - BSA and Azide free (ab271853)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
Datasheet download
Certificate of Compliance
文献 (0)
ab271853 尚未被引用在任何文献中。
