Anti-Poly (ADP-Ribose) Polymer抗体(ab14460)
Key features and details
- Chicken polyclonal to Poly (ADP-Ribose) Polymer
- Suitable for: ICC/IF, IHC-Fr, WB, ELISA, IHC-P
- Reacts with: Human, Species independent
- Isotype: IgY
概述
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产品名称
Anti-Poly (ADP-Ribose) Polymer抗体
参阅全部 Poly (ADP-Ribose) Polymer 一抗 -
描述
鸡多克隆抗体to Poly (ADP-Ribose) Polymer -
宿主
Chicken -
特异性
This antibody recognizes Poly (ADP-Ribose) Polymer synthesized by a variety of poly(ADP-ribose) polymerases (PARP)-related enzymes, including PARP1, 2, 3, tankyrase, vPARP, sPARP and others. It does not cross-react with ADP-ribose, 5'-AMP, or yeast RNA as tested by ELISA. It does cross-react to bovine serum albumin due to its use as a carrier for the immunogen. -
经测试应用
适用于: ICC/IF, IHC-Fr, WB, ELISA, IHC-Pmore details -
种属反应性
与反应: Human, Species independent -
免疫原
Other Immunogen Type corresponding to Poly (ADP-Ribose) Polymer. Purified Poly (ADP-Ribose) Polymer mixed with methylated bovine serum albumin.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.316% Tris HCl, 0.87% Sodium chloride -
Concentration information loading... -
纯度
IgY fraction -
克隆
多克隆 -
同种型
IgY -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab14460于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | (1) |
1/200.
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| IHC-Fr |
Use at an assay dependent concentration.
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| WB | (1) |
Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 1 kDa.
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| ELISA |
Use at an assay dependent concentration. It is recommended to start with a 1/100 dilution.
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| IHC-P |
Use at an assay dependent concentration.
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| 说明 |
|---|
|
ICC/IF
1/200. |
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IHC-Fr
Use at an assay dependent concentration. |
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WB
Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 1 kDa. |
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ELISA
Use at an assay dependent concentration. It is recommended to start with a 1/100 dilution. |
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IHC-P
Use at an assay dependent concentration. |
靶标
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相关性
Poly (ADP-Ribose) is a polymer synthesized by a class of enzymes named poly(ADP-ribose) polymerases (PARP). Using NAD+ as substrate, PARP catalyzes the formation of the polymer poly (ADP-Ribose), with chain lengths ranging from 2 to 300 residues, containing approximately 2% branching in the chain. Poly (ADP-Ribose) polymer becomes attached to nuclear proteins, and to PARP itself (automodification). Under normal conditions, cells display low basal level of poly (ADP-Ribose) polymer, which can dramatically increase in cells exposed to DNA damaging agents (irradiation, alkylation, etc.). This increase of polymer synthesis is usually transient and is followed by a rapid degradation phase with a short half life which can be less than 1 min. The low endogenous level of polymer in unstimulated cells and its rapid catabolism during DNA damage has been ascribed to high activity of the polymer catabolizing enzyme poly(ADP-ribose) glycohydrolyase (PARG). -
别名
- pADPr antibody
- Poly ADP ribose antibody
图片
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Immunohistochemistry (Frozen sections) - Anti-Poly (ADP-Ribose) Polymer antibody (ab14460)
Poly (ADP-Ribose) Polymer staining of untreated rat liver, with ab14460 at a concentration of 20µg/ml.
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Western blot - Anti-Poly (ADP-Ribose) Polymer antibody (ab14460)Western blot analysis of Poly (ADP-Ribose) Polymer. Lane 1: control HL60 cells (75,000 cells). Lane 2: cells automodified with PARP1. Note the apparent shift in molecular weight PARP1 from 116kDa to a broad range >250kDa due to various extents of Poly (ADP-Ribose) Polymer automodification. ab14460 was used at a concentration of 10 µg/ml.
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Immunohistochemistry (Frozen sections) - Anti-Poly (ADP-Ribose) Polymer antibody (ab14460)Poly (ADP-Ribose) Polymer staining of livers from rats injected with diethylnitrosamine (200 mg/kg). The livers were removed and rapidly processed 10 hr later, at peak polymer induction. ab14460 was used at a concentration of 20µg/ml
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Immunocytochemistry/ Immunofluorescence - Anti-Poly (ADP-Ribose) Polymer antibody (ab14460)This image is courtesy of an Abreview submitted by Dr Alexander Rappab14460 staining cultured human HeLa cells by ICC/IF. Cells were PFA fixed and permeabilized in 0.5% Triton X100 prior to blocking in 5% BSA for 1 hour at 20°C. The primary antibody was diluted 1/200 and incubated with the sample for 1 hour at 20°C. A Cy5® conjugated donkey anti-chicken antibody diluted 1/300 was used as the secondary.
实验方案
数据表及文件
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Datasheet download
文献 (17)
ab14460 被引用在 17 文献中.
- Wu H et al. Crocetin antagonizes parthanatos in ischemic stroke via inhibiting NOX2 and preserving mitochondrial hexokinase-I. Cell Death Dis 14:50 (2023). PubMed: 36681688
- Li F et al. Poly (ADP-ribose) polymerase 1 (PARP1) inhibition promotes pulmonary metastasis of osteosarcoma by boosting ezrin phosphorylation. Int J Biol Sci 18:1238-1253 (2022). PubMed: 35173550
- Maynard S et al. Lamin A/C impairments cause mitochondrial dysfunction by attenuating PGC1α and the NAMPT-NAD+ pathway. Nucleic Acids Res 50:9948-9965 (2022). PubMed: 36099415
- Zhong L et al. Dual inhibition of VEGF and PARP suppresses KRAS-mutant colorectal cancer. Neoplasia 22:365-375 (2020). PubMed: 32629177
- Marchetta P et al. Guanylyl Cyclase A/cGMP Signaling Slows Hidden, Age- and Acoustic Trauma-Induced Hearing Loss. Front Aging Neurosci 12:83 (2020). PubMed: 32327991
