AB14460

Anti-Poly (ADP-Ribose) Polymer抗体

Anti-Poly (ADP-Ribose) Polymer antibody

(2 Reviews)

(19 Publications)

Chicken Polyclonal Poly (ADP-Ribose) Polymer antibody. Suitable for ELISA, WB and reacts with Chemical samples. Cited in 19 publications.

查看别名

Poly ADP ribose, pADPr

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Western blot - Anti-Poly (ADP-Ribose) Polymer antibody (AB14460)

Western blot analysis of Poly (ADP-Ribose) Polymer. Lane 1 : control HL60 cells (75,000 cells). Lane 2 : cells automodified with PARP1. Note the apparent shift in molecular weight PARP1 from 116kDa to a broad range >250kDa due to various extents of Poly (ADP-Ribose) Polymer automodification. ab14460 was used at a concentration of 10 μg/ml.

All lanes:

Western blot - Anti-Poly (ADP-Ribose) Polymer antibody (ab14460)

Predicted band size: 1 kDa

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关键信息

宿主种属

Chicken

克隆

Polyclonal

亚型

IgY

不含载体蛋白

应用

ELISA, WB

applications

特异性

This antibody recognizes Poly (ADP-Ribose) Polymer synthesized by a variety of poly(ADP-ribose) polymerases (PARP)-related enzymes, including PARP1, 2, 3, tankyrase, vPARP, sPARP and others. It does not cross-react with ADP-ribose, 5'-AMP, or yeast RNA as tested by ELISA. It does cross-react to bovine serum albumin due to its use as a carrier for the immunogen.

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反应性数据

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性能和储存信息

形式

Liquid

纯度

IgY fraction

存储溶液

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 0.87% Sodium chloride, 0.316% Tris HCl

运输条件

Blue Ice

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Poly (ADP-Ribose) Polymerase often abbreviated as PARP is an enzyme that plays an important role in cellular processes. PARP catalyzes the addition of ADP-ribose units from NAD+ to target proteins—a process known as poly(ADP-ribosyl)ation. This modification is important for modulating protein function. The enzyme has a molecular weight of approximately 116 kDa. PARP is located mainly in the nucleus of cells and shows high expression in tissues with elevated cellular proliferation and metabolism such as the brain and testis.

Biological function summary

PARP is integral to the DNA damage response mechanism in eukaryotic cells. It detects and signals single-strand DNA breaks inducing repair by recruiting DNA repair complexes. PARP collaborates with XRCC1 to facilitate the base excision repair pathway. As a part of these complexes PARP’s activity ensures cell survival under stress conditions. In concert with other poly (ADP-ribose) molecules PARP also influences chromatin structure impacting gene expression.

Pathways

PARP functions prominently within the signaling responses to DNA damage and apoptosis pathways. It associates closely with proteins like ATM and ATR as they coordinate cell cycle checkpoints. PARP’s enzymatic activity modulates the synthesis of poly (ADP-ribose) which interacts with other repair proteins to drive DNA strand repair. Furthermore the activity of PARP connects to the tumor suppressor protein p53 influencing cellular fate decisions between cell survival and apoptosis.

PARP is heavily studied in the context of cancer and neurodegenerative diseases. Elevated PARP activity is recognized in various cancers as it helps tumor cells survive DNA damage. PARP inhibitors have emerged as therapeutic agents targeting cancers with BRCA1/2 mutations. Moreover in neurodegenerative diseases like Parkinson’s PARP’s excessive activation can lead to cell death. The interaction of PARP with other proteins such as α-synuclein in Parkinson’s highlights its multifaceted role in disease pathogenesis.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

See full target information Poly (ADP-Ribose) Polymer

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文献 (19)

Recent publications for all applications. Explore the full list and refine your search

Breast cancer research : BCR 27:138 PubMed40713647

2025

Short-term consumption of the modified standard American diet perturbed the metabolic balance and altered DNA damage in MMTV-PyMT transgenic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Arlet Hernandez,Alekhya Puppala,Jenna Hedlich-Dwyer,Nayonika Mukherjee,Guihua Zhai,Valeria L Dal Zotto,Bohan Ning,Hua Guo,Ritu Aneja,Natalie R Gassman

Cell death & disease 14:50 PubMed36681688

2023

Crocetin antagonizes parthanatos in ischemic stroke via inhibiting NOX2 and preserving mitochondrial hexokinase-I.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Wu,Ying Li,Qian Zhang,Hanxun Wang,Wenyu Xiu,Pu Xu,Yujie Deng,Wanxu Huang,Dan Ohtan Wang

Nucleic acids research 50:9948-9965 PubMed36099415

2022

Lamin A/C impairments cause mitochondrial dysfunction by attenuating PGC1α and the NAMPT-NAD+ pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Scott Maynard,Arnaldur Hall,Panagiotis Galanos,Salvatore Rizza,Tatsuro Yamamoto,Helena Hagner Gram,Sebastian H N Munk,Muhammad Shoaib,Claus Storgaard Sørensen,Vilhelm A Bohr,Mads Lerdrup,Apolinar Maya-Mendoza,Jiri Bartek

International journal of biological sciences 18:1238-1253 PubMed35173550

2022

Poly (ADP-ribose) polymerase 1 (PARP1) inhibition promotes pulmonary metastasis of osteosarcoma by boosting ezrin phosphorylation.

Applications

Unspecified application

Species

Unspecified reactive species

Fangfei Li,Xiaoqiu Wu,Xuekun Fu,Jin Liu,Wangze Song,Gary Guishan Xiao,Aiping Lu,Ge Zhang

Neoplasia (New York, N.Y.) 22:365-375 PubMed32629177

2020

Dual inhibition of VEGF and PARP suppresses KRAS-mutant colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Longhui Zhong,Rong Wang,Yanxia Wang,Shunli Peng,Yueyun Ma,Sijie Ding,Hong Yang,Shiyu Chen,Xiaoqing Luo,Wei Wang

Frontiers in aging neuroscience 12:83 PubMed32327991

2020

Guanylyl Cyclase A/cGMP Signaling Slows Hidden, Age- and Acoustic Trauma-Induced Hearing Loss.

Applications

Unspecified application

Species

Unspecified reactive species

Philine Marchetta,Dorit Möhrle,Philipp Eckert,Katrin Reimann,Steffen Wolter,Arianna Tolone,Isabelle Lang,Markus Wolters,Robert Feil,Jutta Engel,François Paquet-Durand,Michaela Kuhn,Marlies Knipper,Lukas Rüttiger

DNA repair 85:102749 PubMed31790865

2019

Shining light on the response to repair intermediates in DNA of living cells.

Applications

Unspecified application

Species

Unspecified reactive species

Agnes K Janoshazi,Julie K Horton,Ming-Lang Zhao,Rajendra Prasad,Erica L Scappini,C Jeff Tucker,Samuel H Wilson

Nucleic acids research 47:11709-11728 PubMed31647095

2019

Lamin A/C promotes DNA base excision repair.

Applications

Unspecified application

Species

Unspecified reactive species

Scott Maynard,Guido Keijzers,Mansour Akbari,Michael Ben Ezra,Arnaldur Hall,Marya Morevati,Morten Scheibye-Knudsen,Susana Gonzalo,Jiri Bartek,Vilhelm A Bohr

PloS one 14:e0223725 PubMed31596905

2019

Defective base excision repair in the response to DNA damaging agents in triple negative breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Kevin J Lee,Cortt G Piett,Joel F Andrews,Elise Mann,Zachary D Nagel,Natalie R Gassman

Journal of cellular and molecular medicine 23:6797-6804 PubMed31338966

2019

Berberine attenuates XRCC1-mediated base excision repair and sensitizes breast cancer cells to the chemotherapeutic drugs.

Applications

Unspecified application

Species

Unspecified reactive species

Xingjie Gao,Jing Wang,Meiqi Li,Jia Wang,Jian Lv,Lu Zhang,Caifeng Sun,Jiamei Ji,Wenbo Yang,Zinan Zhao,Weifeng Mao

View all publications

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Anti-Poly (ADP-Ribose) Polymer抗体

Anti-Poly (ADP-Ribose) Polymer antibody

Western blot - Anti-Poly (ADP-Ribose) Polymer antibody (AB14460)