Anti-Poly (ADP-Ribose) Polymer抗体[10H] (ab14459)
![Immunohistochemistry (Frozen sections) - Anti-Poly (ADP-Ribose) Polymer antibody [10H] (ab14459)](https://www.abcam.cn/ps/products/14/ab14459/Images/ab14459-21939-anti-poly-adp-ribose-polymer-antibody-10h-immunohistochemistry.jpg "Immunohistochemistry (Frozen sections) - Anti-Poly (ADP-Ribose) Polymer antibody [10H] (ab14459)")
Key features and details
- Mouse monoclonal [10H] to Poly (ADP-Ribose) Polymer
- Suitable for: IHC-Fr
- Reacts with: Species independent
- Isotype: IgG3
概述
-
产品名称
Anti-Poly (ADP-Ribose) Polymer抗体[10H]
参阅全部 Poly (ADP-Ribose) Polymer 一抗 -
描述
小鼠单克隆抗体[10H] to Poly (ADP-Ribose) Polymer -
宿主
Mouse -
特异性
This antibody reacts with poly (ADP-Ribose) Polymer synthesized by a variety of poly(ADP- ribose) polymerases (PARP)-related enzymes including PARP1, 2, 3, tankyrase, vPARP, sPARP and others. The antibody does not cross-react with ADP-ribose, 5'-AMP, or yeast RNA as tested by ELISA. -
经测试应用
适用于: IHC-Frmore details -
种属反应性
与反应: Species independent -
免疫原
Other Immunogen Type conjugated to bovine serum albumin. Poly (ADP-Ribose) polymer mixed with methylated bovine serum albumin.
-
阳性对照
- Rat liver induced for Poly (ADP-Ribose) Polymer synthesis by injection with diethylnitrosamine. MEF's treated with 500um Hydrogen Peroxide.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.242% Tris, 0.87% Sodium chloride, 1% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
10H -
骨髓瘤
NS1 -
同种型
IgG3 -
轻链类型
kappa -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab14459于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-Fr | (1) |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
IHC-Fr
Use at an assay dependent concentration. |
靶标
-
相关性
Poly (ADP-Ribose) is a polymer synthesized by a class of enzymes named poly(ADP-ribose) polymerases (PARP). Using NAD+ as substrate, PARP catalyzes the formation of the polymer poly (ADP-Ribose), with chain lengths ranging from 2 to 300 residues, containing approximately 2% branching in the chain. Poly (ADP-Ribose) polymer becomes attached to nuclear proteins, and to PARP itself (automodification). Under normal conditions, cells display low basal level of poly (ADP-Ribose) polymer, which can dramatically increase in cells exposed to DNA damaging agents (irradiation, alkylation, etc.). This increase of polymer synthesis is usually transient and is followed by a rapid degradation phase with a short half life which can be less than 1 min. The low endogenous level of polymer in unstimulated cells and its rapid catabolism during DNA damage has been ascribed to high activity of the polymer catabolizing enzyme poly(ADP-ribose) glycohydrolyase (PARG). -
别名
- pADPr antibody
- Poly ADP ribose antibody
图片
-
Immunohistochemistry (Frozen sections) - Anti-Poly (ADP-Ribose) Polymer antibody [10H] (ab14459)Immunohistochemistry of rat livers treated with diethylnitrosamine (200 mg/kg) and stained with ab14459 diluted 1/100. After treatment livers were removed and rapidly processed 10 hr later, at peak polymer induction. Left hand side image was from diethylnitrosamine untreated liver tissue and right one represents DEN treated sections.
数据表及文件
-
SDS download
-
Datasheet download
文献 (62)
ab14459 被引用在 62 文献中.
- Andreeva TV et al. Epigallocatechin Gallate Affects the Structure of Chromatosomes, Nucleosomes and Their Complexes with PARP1. Int J Mol Sci 24:N/A (2023). PubMed: 37762491
- Morgan SM et al. The three-dimensional structure of Epstein-Barr virus genome varies by latency type and is regulated by PARP1 enzymatic activity. Nat Commun 13:187 (2022). PubMed: 35039491
- Jhanji M et al. Cis- and trans-resveratrol have opposite effects on histone serine-ADP-ribosylation and tyrosine induced neurodegeneration. Nat Commun 13:3244 (2022). PubMed: 35688816
- Santofimia-Castaño P et al. NUPR1 protects against hyperPARylation-dependent cell death. Commun Biol 5:732 (2022). PubMed: 35869257
- Zhang W et al. Role of PARP-1 in Human Cytomegalovirus Infection and Functional Partners Encoded by This Virus. Viruses 14:N/A (2022). PubMed: 36146855