AB14459

Anti-Poly (ADP-Ribose) Polymer 抗体 [10H]

Anti-Poly (ADP-Ribose) Polymer antibody [10H]

(15 Reviews)

(76 Publications)

Anti-Poly (ADP-Ribose) Polymer antibody [10H] (ab14459) is a mouse monoclonal antibody detecting Poly (ADP-Ribose) Polymer in IHC-Fr.

- Over 60 publications
- Trusted since 2004

查看别名

Poly ADP ribose, pADPr

1 Images

Immunohistochemistry (Frozen sections) - Anti-Poly (ADP-Ribose) Polymer antibody [10H] (AB14459)

IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-Poly (ADP-Ribose) Polymer antibody [10H] (AB14459)

Immunohistochemistry of rat livers treated with diethylnitrosamine (200 mg/kg) and stained with ab14459 diluted 1/100. After treatment livers were removed and rapidly processed 10 hr later, at peak polymer induction. Left hand side image was from diethylnitrosamine untreated liver tissue and right one represents DEN treated sections.

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

10H

亚型

IgG3

轻链类型

kappa

不含载体蛋白

应用

IHC-Fr

applications

特异性

This antibody reacts with poly (ADP-Ribose) Polymer synthesized by a variety of poly(ADP- ribose) polymerases (PARP)-related enzymes including PARP1, 2, 3, tankyrase, vPARP, sPARP and others. The antibody does not cross-react with ADP-ribose, 5'-AMP, or yeast RNA as tested by ELISA.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Chemical": { "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" } } }

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产品详情

What is this antibody validated in?
Anti-Poly (ADP-Ribose) Polymer antibody [10H] (ab14459) is a mouse monoclonal antibody and is validated for use in Immunohistochemistry (IHC-Fr). Trusted by the scientific community
Anti-Poly (ADP-Ribose) Polymer [10H] (ab14459) was first used in a scientific publication in 2004 and has been cited over 60 times in peer-reviewed journals.

Reviewed by scientists
Anti-Poly (ADP-Ribose) Polymer [10H] (ab14459) has over 10 independent reviews from customers.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 1% BSA, 0.87% Sodium chloride, 0.242% Tris

运输条件

Blue Ice

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Poly (ADP-Ribose) Polymerase often abbreviated as PARP is an enzyme that plays an important role in cellular processes. PARP catalyzes the addition of ADP-ribose units from NAD+ to target proteins—a process known as poly(ADP-ribosyl)ation. This modification is important for modulating protein function. The enzyme has a molecular weight of approximately 116 kDa. PARP is located mainly in the nucleus of cells and shows high expression in tissues with elevated cellular proliferation and metabolism such as the brain and testis.

Biological function summary

PARP is integral to the DNA damage response mechanism in eukaryotic cells. It detects and signals single-strand DNA breaks inducing repair by recruiting DNA repair complexes. PARP collaborates with XRCC1 to facilitate the base excision repair pathway. As a part of these complexes PARP’s activity ensures cell survival under stress conditions. In concert with other poly (ADP-ribose) molecules PARP also influences chromatin structure impacting gene expression.

Pathways

PARP functions prominently within the signaling responses to DNA damage and apoptosis pathways. It associates closely with proteins like ATM and ATR as they coordinate cell cycle checkpoints. PARP’s enzymatic activity modulates the synthesis of poly (ADP-ribose) which interacts with other repair proteins to drive DNA strand repair. Furthermore the activity of PARP connects to the tumor suppressor protein p53 influencing cellular fate decisions between cell survival and apoptosis.

PARP is heavily studied in the context of cancer and neurodegenerative diseases. Elevated PARP activity is recognized in various cancers as it helps tumor cells survive DNA damage. PARP inhibitors have emerged as therapeutic agents targeting cancers with BRCA1/2 mutations. Moreover in neurodegenerative diseases like Parkinson’s PARP’s excessive activation can lead to cell death. The interaction of PARP with other proteins such as α-synuclein in Parkinson’s highlights its multifaceted role in disease pathogenesis.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

See full target information Poly (ADP-Ribose) Polymer

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文献 (76)

Recent publications for all applications. Explore the full list and refine your search

Cells 14: PubMed40940725

2025

Non-Classical H1-like PARP1 Binding to Chromatosome.

Applications

Unspecified application

Species

Unspecified reactive species

Daria Koshkina,Natalya Maluchenko,Dmitry Nilov,Alexander Lyubitelev,Anna Korovina,Sergey Pushkarev,Grigoriy Armeev,Mikhail Kirpichnikov,Vasily Studitsky,Alexey Feofanov

JACC. Advances 4:102013 PubMed40712271

2025

Assessing Cardiomyocyte DNA Damage Using Cardiovascular Magnetic Resonance T Mapping: Insights From Comparative Biopsy Analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Mizuki Ichikawa,Shiro Nakamori,Masaki Ishida,Ryuji Okamoto,Rie Ito,Junko Ishiura,Masaki Ishiyama,Taku Omori,Keishi Moriwaki,Emiyo Sugiura,Naoki Fujimoto,Tairo Kurita,Yasutaka Ichikawa,Kakuya Kitagawa,Kyoko Imanaka-Yoshida,Hajime Sakuma,Kaoru Dohi

IUBMB life 77:e70030 PubMed40476370

2025

Tyrosine and Phenylalanine Activate Neuronal DNA Repair but Exhibit Opposing Effects on Global Transcription and Adult Female Mice Are Resilient to TyrRS/YARS1 Depletion.

Applications

Unspecified application

Species

Unspecified reactive species

Megha Jhanji,Ashita Bhan,Colin Arrowood,Dina W Yakout,Ankit Shroff,Danielle McManus,Henrietta Gifford,Janay Vacharasin,Sofia B Lizarraga,Taras Y Nazarko,Angela M Mabb,Mathew Sajish

Clinical cancer research : an official journal of the American Association for Cancer Research 31:2347-2357 PubMed40358364

2025

Cambridge Neoadjuvant Cancer of the Prostate (CANCAP03): A Window Study into the Effects of Olaparib ± Degarelix in Primary Prostate Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Harveer Dev,Mark Linch,Krishna Narahari,Toby Milne-Clark,Melissa Cheung,Anne Warren,Alopa Malaviya,Vincent Gnanapragasam,Tatiana Hernandez,Nicholas Bullock,Andrea Machin,Alimu Dayimu,Tamsin Robb,Elizabeth Cromwell,Alex Freeman,Elizabeth A Harrington,Niedzika Camacho,Silvia Glont,Massimo Squatrito,Asaf Rotem,Luiza Moore,Robert Hanson,Marc Dodd,Shubha Anand,Howard Kynaston,Greg Shaw,Nimish Shah,Simon Pacey

Journal of cell science 138: PubMed40135584

2025

γ-tubulin mediates DNA double-strand break repair.

Applications

Unspecified application

Species

Unspecified reactive species

Abhishikt David Solomon,Odjo G Gouttia,Ling Wang,Songli Zhu,Feifei Wang,Yanqui Li,Mohammadjavad Paydar,Tadayoshi Bessho,Benjamin H Kwok,Aimin Peng

Experimental & molecular medicine 57:264-280 PubMed39870799

2025

RECQL4 requires PARP1 for recruitment to DNA damage, and PARG dePARylation facilitates its associated role in end joining.

Applications

Unspecified application

Species

Unspecified reactive species

Mansoor Hussain,Prabhat Khadka,Komal Pekhale,Tomasz Kulikowicz,Samuel Gray,Alfred May,Deborah L Croteau,Vilhelm A Bohr

Biomolecules 14: PubMed39595575

2024

Resveratrol Inhibits Nucleosome Binding and Catalytic Activity of PARP1.

Applications

Unspecified application

Species

Unspecified reactive species

Daria O Koshkina,Natalya V Maluchenko,Anna N Korovina,Angelina A Lobanova,Alexey V Feofanov,Vasily M Studitsky

Veterinary sciences 11: PubMed39330799

2024

Spermiogram, Kinetics, Flow Cytometric Characteristics and DNA Damage Degree in Boar Ejaculates: Summarization and Clustering.

Applications

Unspecified application

Species

Unspecified reactive species

Raquel Ausejo-Marcos,María Teresa Tejedor,Sara Miguel-Jiménez,Belén Gómez-Giménez,Cristina Soriano-Úbeda,Noelia Mendoza,Alejandro Vicente-Carrillo,William Fernando Hurtado,Celia Ávila Holguín,Bernardino Moreno,María Victoria Falceto

Nucleic acids research 52:12351-12377 PubMed39315696

2024

p53-dependent crosstalk between DNA replication integrity and redox metabolism mediated through a NRF2-PARP1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Gamal Ahmed Elfar,Obed Aning,Tsz Wai Ngai,Pearlyn Yeo,Joel Wai Kit Chan,Shang Hong Sim,Leonard Goh,Ju Yuan,Cheryl Zi Jin Phua,Joanna Zhen Zhen Yeo,Shi Ya Mak,Brian Kim Poh Goh,Pierce Kah-Hoe Chow,Wai Leong Tam,Ying Swan Ho,Chit Fang Cheok

Redox biology 73:103187 PubMed38744190

2024

Central carbon metabolism exhibits unique characteristics during the handling of fungal patterns by monocyte-derived dendritic cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yolanda Alvarez,Cristina Mancebo,Sara Alonso,Olimpio Montero,Nieves Fernández,Mariano Sánchez Crespo

View all publications

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Anti-Poly (ADP-Ribose) Polymer 抗体 [10H]

Anti-Poly (ADP-Ribose) Polymer antibody [10H]

Immunohistochemistry (Frozen sections) - Anti-Poly (ADP-Ribose) Polymer antibody [10H] (AB14459)

关键信息

宿主种属

克隆

克隆号

亚型

轻链类型

不含载体蛋白

应用

特异性