Anti-POLR1C抗体(ab224621)
Key features and details
- Rabbit polyclonal to POLR1C
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-POLR1C抗体
参阅全部 POLR1C 一抗 -
描述
兔多克隆抗体to POLR1C -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Cow
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免疫原
Recombinant fragment corresponding to Human POLR1C aa 100-250.
Database link: O15160 -
阳性对照
- WB: HEK-293, K562, NIH/3T3, HepG2 and RAW 264.7 whole cell lysates; Mouse liver and kidney tissue lysates; Rat heart tissue lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
纯度
Protein G purified -
纯化说明
Purity >95%. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab224621于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/5000. Detects a band of approximately 40 kDa (predicted molecular weight: 39,40 kDa).
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| 说明 |
|---|
|
WB
1/500 - 1/5000. Detects a band of approximately 40 kDa (predicted molecular weight: 39,40 kDa). |
靶标
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功能
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I and III which synthesize ribosomal RNA precursors and small RNAs, such as 5S rRNA and tRNAs, respectively. RPAC1 is part of the Pol core element with the central large cleft and probably a clamp element that moves to open and close the cleft. -
疾病相关
Treacher Collins syndrome 3 (TCS3) [MIM:248390]: A form of Treacher Collins syndrome, a disorder of craniofacial development. Treacher Collins syndrome is characterized by a combination of bilateral downward slanting of the palpebral fissures, colobomas of the lower eyelids with a paucity of eyelashes medial to the defect, hypoplasia of the facial bones, cleft palate, malformation of the external ears, atresia of the external auditory canals, and bilateral conductive hearing loss. Note=The disease is caused by mutations affecting the gene represented in this entry. -
序列相似性
Belongs to the archaeal RpoD/eukaryotic RPB3 RNA polymerase subunit family. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 516337 Cow
- Entrez Gene: 9533 Human
- Entrez Gene: 20016 Mouse
- Entrez Gene: 301246 Rat
- Omim: 610060 Human
- SwissProt: Q32L22 Cow
- SwissProt: O15160 Human
- SwissProt: P52432 Mouse
see all -
别名
- 40kDa antibody
- AA409007 antibody
- AA959927 antibody
see all
图片
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Western blot - Anti-POLR1C antibody (ab224621)All lanes : Anti-POLR1C antibody (ab224621) at 1/500 dilution
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
Lane 2 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 3 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 5 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 6 : Mouse liver tissue lysate
Lane 7 : Mouse kidney tissue lysate
Lane 8 : Rat heart tissue lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 39,40 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab224621 尚未被引用在任何文献中。