Anti-Pofut1抗体(ab74302)
Key features and details
- Rabbit polyclonal to Pofut1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Pofut1抗体
参阅全部 Pofut1 一抗 -
描述
兔多克隆抗体to Pofut1 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Rat
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免疫原
Synthetic peptide derived from an internal region of human Pofat1.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab74302于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/1000. Predicted molecular weight: 44 kDa.
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|
| IHC-P |
1/50 - 1/100.
|
| 说明 |
|---|
|
WB
1/500 - 1/1000. Predicted molecular weight: 44 kDa. |
|
IHC-P
1/50 - 1/100. |
靶标
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功能
Catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue in EGF domains. Plays a crucial role in Notch signaling. -
组织特异性
Highly expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. -
通路
Protein modification; protein glycosylation. -
序列相似性
Belongs to the glycosyltransferase 68 family. -
细胞定位
Endoplasmic reticulum. - Information by UniProt
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数据库链接
- Entrez Gene: 23509 Human
- Entrez Gene: 311551 Rat
- Omim: 607491 Human
- SwissProt: Q9H488 Human
- SwissProt: Q6EV70 Rat
- Unigene: 472409 Human
- Unigene: 214685 Rat
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别名
- FUT 12 antibody
- FUT12 antibody
- GDP fucose protein O fucosyltransferase 1 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pofut1 antibody (ab74302)ab74302, at a 1/50 dilution, staining Pofut1 in paraffin embedded human lung carcinoma tissue in the absence (left image) and presence (right image) of the immunising peptide.
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Western blot - Anti-Pofut1 antibody (ab74302)All lanes : Anti-Pofut1 antibody (ab74302) at 1/500 dilution
Lane 1 : extracts from HeLa cells
Lane 2 : extracts from Jurkat cells
Lane 3 : extracts from HepG2 cells
Lane 4 : extracts from Jurkat cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 44 kDa
Observed band size: 44 kDa
Additional bands at: 117 kDa, 22 kDa, 28 kDa, 30 kDa. We are unsure as to the identity of these extra bands.
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab74302 被引用在 3 文献中.
- Wang P et al. Fucosyltransferases Regulated by Fusobacterium Nucleatum and Act as Novel Biomarkers in Colon Adenocarcinoma. J Inflamm Res 16:747-768 (2023). PubMed: 36852302
- Wahby S et al. POFUT1 mRNA expression as an independent prognostic parameter in muscle-invasive bladder cancer. Transl Oncol 14:100900 (2021). PubMed: 33099185
- Chabanais J et al. POFUT1 as a Promising Novel Biomarker of Colorectal Cancer. Cancers (Basel) 10:N/A (2018). PubMed: 30380753