重组Anti-Podoplanin抗体[EPR7073] (ab131216)
![Western blot - Rabbit monoclonal [EPR7073] to Podoplanin (ab131216)](https://www.abcam.cn/ps/products/131/ab131216/Images/ab131216-145519-anti-podoplanin-gp36-antibody-epr7073-western-blot.jpg "Western blot - Rabbit monoclonal [EPR7073] to Podoplanin (ab131216)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7073] to Podoplanin
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Podoplanin抗体[EPR7073]
参阅全部 Podoplanin 一抗 -
描述
兔单克隆抗体[EPR7073] to Podoplanin -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,ICC/IF,IHC-P or IP -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Human tonsil, uterus cancer, placenta tissue, HT 1080 and fetal lung lysates.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数（KD）
KD = 3.30 x 10 -11 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR7073 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab131216于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 36-45 kDa (predicted molecular weight: 16 kDa).
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| 说明 |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 36-45 kDa (predicted molecular weight: 16 kDa). |
应用说明
Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
靶标
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功能
May be involved in cell migration and/or actin cytoskeleton organization. When expressed in keratinocytes, induces changes in cell morphology with transfected cells showing an elongated shape, numerous membrane protrusions, major reorganization of the actin cytoskeleton, increased motility and decreased cell adhesion. Required for normal lung cell proliferation and alveolus formation at birth. Induces platelet aggregation. Does not have any effect on folic acid or amino acid transport. Does not function as a water channel or as a regulator of aquaporin-type water channels. -
组织特异性
Highly expressed in placenta, lung, skeletal muscle and brain. Weakly expressed in brain, kidney and liver. In placenta, expressed on the apical plasma membrane of endothelium. In lung, expressed in alveolar epithelium. Up-regulated in colorectal tumors and expressed in 25% of early oral squamous cell carcinomas. -
序列相似性
Belongs to the podoplanin family. -
翻译后修饰
Extensively O-glycosylated. Contains sialic acid residues. O-glycosylation is necessary for platelet aggregation activity.
The N-terminus is blocked. -
细胞定位
Membrane. Cell projection > filopodium membrane. Cell projection > lamellipodium membrane. Cell projection > microvillus membrane. Cell projection > ruffle membrane. Localized to actin-rich microvilli and plasma membrane projections such as filopodia, lamellipodia and ruffles. - Information by UniProt
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数据库链接
- Entrez Gene: 10630 Human
- Entrez Gene: 14726 Mouse
- Entrez Gene: 54320 Rat
- Omim: 608863 Human
- SwissProt: Q86YL7 Human
- SwissProt: Q62011 Mouse
- SwissProt: Q64294 Rat
- Unigene: 468675 Human
see all -
别名
- Aggrus antibody
- D2-40 antibody
- Glycoprotein 36 KD antibody
see all
图片
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Western blot - Rabbit monoclonal [EPR7073] to Podoplanin (ab131216)All lanes : Anti-Podoplanin antibody [EPR7073] (ab131216) at 1/1000 dilution
Lane 1 : Human fetal lung lysate
Lane 2 : Human tonsil lysate
Lane 3 : Human uterus cancer lysate
Lane 4 : Human placenta lysate
Lane 5 : HT 1080 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 16 kDa -
![OI-RD Scanning - Rabbit monoclonal [EPR7073] to Podoplanin (ab131216)](https://www.abcam.cn/ps/products/131/ab131216/Images/ab131216-145518-anti-podoplanin-gp36-antibody-epr7073.jpg)
OI-RD Scanning - Rabbit monoclonal [EPR7073] to Podoplanin (ab131216) -
![Rabbit monoclonal [EPR7073] to Podoplanin (ab131216)](https://www.abcam.cn/ps/products/131/ab131216/Images/ab131216-3-benefits-of-recombinant-antibodies.png)
Rabbit monoclonal [EPR7073] to Podoplanin (ab131216)
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab131216 被引用在 3 文献中.
- Chang JH et al. Air pollution-regulated E-cadherin mediates contact inhibition of proliferation via the hippo signaling pathways in emphysema. Chem Biol Interact 351:109763 (2022). PubMed: 34852269
- Fang Q et al. pre-B cell colony enhancing factor negatively regulates Na+ and fluid transport in lung epithelial cells. Am J Transl Res 10:2047-2054 (2018). PubMed: 30093942
- Xu Y et al. Polypeptide N-Acetylgalactosaminyltransferase 13 Contributes to Neurogenesis via Stabilizing the Mucin-type O-Glycoprotein Podoplanin. J Biol Chem 291:23477-23488 (2016). PubMed: 27629416