Anti-Podoplanin抗体(ab10274)
Key features and details
- Rabbit polyclonal to Podoplanin
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Podoplanin抗体
参阅全部 Podoplanin 一抗 -
描述
兔多克隆抗体to Podoplanin -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment (His-tag) corresponding to Human Podoplanin aa 1-150.
Database link: Q86YL7 -
阳性对照
- WB: LEC whole cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Lyophilized:The lyophilized IgG is stable at 4°C for at least one month and for more than a year when kept at –20°C. When reconstituted in sterile water to a concentration of >0.5 mg/ml the antibody is stable for at least six months at 2-4°C in the presence of a preservative. Reconstituted antibody can also be aliquoted and stored at –20°C to –70°C for at least 1 year without detectable loss of activity. Avoid repeated freeze-thaw cycles. -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Constituent: PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab10274于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 16 kDa.
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| 说明 |
|---|
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 16 kDa. |
靶标
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功能
May be involved in cell migration and/or actin cytoskeleton organization. When expressed in keratinocytes, induces changes in cell morphology with transfected cells showing an elongated shape, numerous membrane protrusions, major reorganization of the actin cytoskeleton, increased motility and decreased cell adhesion. Required for normal lung cell proliferation and alveolus formation at birth. Induces platelet aggregation. Does not have any effect on folic acid or amino acid transport. Does not function as a water channel or as a regulator of aquaporin-type water channels. -
组织特异性
Highly expressed in placenta, lung, skeletal muscle and brain. Weakly expressed in brain, kidney and liver. In placenta, expressed on the apical plasma membrane of endothelium. In lung, expressed in alveolar epithelium. Up-regulated in colorectal tumors and expressed in 25% of early oral squamous cell carcinomas. -
序列相似性
Belongs to the podoplanin family. -
翻译后修饰
Extensively O-glycosylated. Contains sialic acid residues. O-glycosylation is necessary for platelet aggregation activity.
The N-terminus is blocked. -
细胞定位
Membrane. Cell projection > filopodium membrane. Cell projection > lamellipodium membrane. Cell projection > microvillus membrane. Cell projection > ruffle membrane. Localized to actin-rich microvilli and plasma membrane projections such as filopodia, lamellipodia and ruffles. - Information by UniProt
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数据库链接
- Entrez Gene: 10630 Human
- Omim: 608863 Human
- SwissProt: Q86YL7 Human
- Unigene: 468675 Human
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别名
- Aggrus antibody
- D2-40 antibody
- Glycoprotein 36 KD antibody
see all
图片
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Western blot - Rabbit polyclonal to Podoplanin (ab10274)
Western Blot analysis of Podoplanin expression in human lymphatic endothelial cells (LEC). The total lysate was subjected to SDS-PAGE and subsequent Western analysis with the polyclonal antibody ab10274.
实验方案
数据表及文件
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Datasheet download
文献 (4)
ab10274 被引用在 4 文献中.
- Kwak J et al. Isolation and characterization of tumorspheres from a recurrent pineoblastoma patient: Feasibility of a patient-derived xenograft. Int J Oncol 49:569-78 (2016). PubMed: 27277549
- Carden CP et al. The association of PI3 kinase signaling and chemoresistance in advanced ovarian cancer. Mol Cancer Ther 11:1609-17 (2012). PubMed: 22556379
- Kong BH et al. Isolation of glioma cancer stem cells in relation to histological grades in glioma specimens. Childs Nerv Syst : (2012). PubMed: 23143002
- Link A et al. Association of T-zone reticular networks and conduits with ectopic lymphoid tissues in mice and humans. Am J Pathol 178:1662-75 (2011). PubMed: 21435450
