Anti-Podoplanin抗体[18H5] - BSA and Azide free (ab10288)
![Immunocytochemistry/ Immunofluorescence - Mouse monoclonal [18H5] to Podoplanin - BSA and Azide free (ab10288)](https://www.abcam.cn/ps/products/10/ab10288/Images/ab10288-17869-anti-podoplanin-gp36-antibody-18h5-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Mouse monoclonal [18H5] to Podoplanin - BSA and Azide free (ab10288)")
Key features and details
- Mouse monoclonal [18H5] to Podoplanin - BSA and Azide free
- Suitable for: ICC/IF, WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Related conjugates and formulations
概述
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产品名称
Anti-Podoplanin抗体[18H5] - BSA and Azide free
参阅全部 Podoplanin 一抗 -
描述
小鼠单克隆抗体[18H5] to Podoplanin - BSA and Azide free -
宿主
Mouse -
经测试应用
适用于: ICC/IF, WB, Flow Cytmore details -
种属反应性
与反应: Human -
免疫原
Full length protein corresponding to Podoplanin. MDCK cells stably expressing Podoplanin protein.
Database link: Q86YL7 -
阳性对照
- WB: human lymphatic endothelial cells, human placenta and skeletal muscle tissue lysates. ICC/IF: human dermal lymphatic endothelial Flow Cyt: HCT 116 (Human colorectal carcinoma cell line) whole cell lysate,
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Constituent: PBS -
无载体
是 -
Concentration information loading... -
纯度
Protein G purified -
克隆
单克隆 -
克隆编号
18H5 -
同种型
IgG1 -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab10288于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | (1) |
Use a concentration of 1 - 2 µg/ml.
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| WB | (1) |
Use a concentration of 5 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 24 kDa).
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| Flow Cyt |
Use 1µl for 106 cells.
(methanol fixed cells)
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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| 说明 |
|---|
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ICC/IF
Use a concentration of 1 - 2 µg/ml. |
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WB
Use a concentration of 5 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 24 kDa). |
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Flow Cyt
Use 1µl for 106 cells. (methanol fixed cells)
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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靶标
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功能
May be involved in cell migration and/or actin cytoskeleton organization. When expressed in keratinocytes, induces changes in cell morphology with transfected cells showing an elongated shape, numerous membrane protrusions, major reorganization of the actin cytoskeleton, increased motility and decreased cell adhesion. Required for normal lung cell proliferation and alveolus formation at birth. Induces platelet aggregation. Does not have any effect on folic acid or amino acid transport. Does not function as a water channel or as a regulator of aquaporin-type water channels. -
组织特异性
Highly expressed in placenta, lung, skeletal muscle and brain. Weakly expressed in brain, kidney and liver. In placenta, expressed on the apical plasma membrane of endothelium. In lung, expressed in alveolar epithelium. Up-regulated in colorectal tumors and expressed in 25% of early oral squamous cell carcinomas. -
序列相似性
Belongs to the podoplanin family. -
翻译后修饰
Extensively O-glycosylated. Contains sialic acid residues. O-glycosylation is necessary for platelet aggregation activity.
The N-terminus is blocked. -
细胞定位
Membrane. Cell projection > filopodium membrane. Cell projection > lamellipodium membrane. Cell projection > microvillus membrane. Cell projection > ruffle membrane. Localized to actin-rich microvilli and plasma membrane projections such as filopodia, lamellipodia and ruffles. - Information by UniProt
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数据库链接
- Entrez Gene: 10630 Human
- Omim: 608863 Human
- SwissProt: Q86YL7 Human
- Unigene: 468675 Human
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别名
- Aggrus antibody
- D2-40 antibody
- Glycoprotein 36 KD antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Mouse monoclonal [18H5] to Podoplanin - BSA and Azide free (ab10288)
ab10288 at a 1/500 dilution staining human dermal lymphatic endothelial by Immunocytochemistry. The cells were grown in coverslips and fixed in formalin prior to permeabilization with tritonX-100. The cells were incubated with the primary antibody at 1/500 for 1 hour and then bound antibody was detected using a biotinylated goat anti-mouse antibody. Texas red-streptavidin was used for development.
This image is courtesy of an Abreview submitted by Maria Navarro.
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![Western blot - Mouse monoclonal [18H5] to Podoplanin - BSA and Azide free (ab10288)](https://www.abcam.cn/ps/products/10/ab10288/Images/ab10288-17866-anti-podoplanin-gp36-antibody-18h5-western-blot.jpg)
Western blot - Mouse monoclonal [18H5] to Podoplanin - BSA and Azide free (ab10288)All lanes : Anti-Podoplanin antibody [18H5] - BSA and Azide free (ab10288) at 5 µg/ml
Lane 1 : Human placenta tissue lysate - total protein (ab29745)
Lane 2 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 24 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutes
Podoplanin protein is extensively glycosylated. This might explain the observation of a band at a higher molecular weight than predicted. -
![Flow Cytometry - Mouse monoclonal [18H5] to Podoplanin - BSA and Azide free (ab10288)](https://www.abcam.cn/ps/products/10/ab10288/Images/ab10288-17867-anti-podoplanin-gp36-antibody-18h5-flow-cytometry.jpg)
Flow Cytometry - Mouse monoclonal [18H5] to Podoplanin - BSA and Azide free (ab10288)Overlay histogram showing HCT116 cells stained with ab10288 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab10288, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a slightly decreased signal in HCT116 cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.
Please note that Abcam does not have data for use of this antibody on non-fixed cells. We welcome any customer feedback. -
![Flow Cytometry - Mouse monoclonal [18H5] to Podoplanin - BSA and Azide free (ab10288)](https://www.abcam.cn/ps/products/10/ab10288/Images/ab10288-467729-antipodoplanin--gp36-antibody-18H5--BSA-and-azide-free-flow-cytometry-2.jpg)
Flow Cytometry - Mouse monoclonal [18H5] to Podoplanin - BSA and Azide free (ab10288)Flow cytometric analysis of human primary lymphatic endothelial cells (HDLEC) labeling Podoplanin with ab10288 at 5 µg/mL. Anti-mouse IgG-PE was used as a secondary antibody.
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![Flow Cytometry - Mouse monoclonal [18H5] to Podoplanin - BSA and Azide free (ab10288)](https://www.abcam.cn/ps/products/10/ab10288/Images/ab10288-467727-antipodoplanin--gp36-antibody-18H5--BSA-and-azide-free-flow-cytometry.jpg)
Flow Cytometry - Mouse monoclonal [18H5] to Podoplanin - BSA and Azide free (ab10288)Flow cytometric analysis of primary human dermal microvascular (HDMVEC) and umbilical vein (HUVEC) endothelial cells labeling Podoplanin with ab10288 at 5 µg/mL. The lymphatic endothelial cell marker Podoplanin is not expressed in the blood endothelial cells.
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![Western blot - Mouse monoclonal [18H5] to Podoplanin - BSA and Azide free (ab10288)](https://www.abcam.cn/ps/products/10/ab10288/Images/ab10288-232685-anti-podoplanin-gp36-antibody-18h5-western-blot.jpg)
Western blot - Mouse monoclonal [18H5] to Podoplanin - BSA and Azide free (ab10288)Western blot analysis of Podoplanin expression in human lymphatic endothelial cells (LEC) - lane 1, and HUVECs - lane 2. Total lysate of both cell types were subjected to SDS-PAGE and subsequent western blot analysis with ab10288. The antibody recognizes a protein of about 36 kDa in total lysate from LECs but not from HUVEC.
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![Immunocytochemistry/ Immunofluorescence - Mouse monoclonal [18H5] to Podoplanin - BSA and Azide free (ab10288)](https://www.abcam.cn/ps/products/10/ab10288/Images/ab10288-232686-anti-podoplanin-gp36-antibody-18h5-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Mouse monoclonal [18H5] to Podoplanin - BSA and Azide free (ab10288)Immunocytochemistry/Immunofluorescence analysis of human dermal lymphatic endothelial cells (HDLEC) labelling Podoplanin with ab10288. An Alexa Fluor® 488-conjugated goat anti-mouse was used as the secondary antibody. Nuclei counterstained with DAPI.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (47)
ab10288 被引用在 47 文献中.
- Rosa I et al. Lymphatic Endothelial-to-Myofibroblast Transition: A Potential New Mechanism Underlying Skin Fibrosis in Systemic Sclerosis. Cells 12:N/A (2023). PubMed: 37681927
- Rütsche D et al. Enzymatically Crosslinked Collagen as a Versatile Matrix for In Vitro and In Vivo Co-Engineering of Blood and Lymphatic Vasculature. Adv Mater 35:e2209476 (2023). PubMed: 36724374
- Zhao S et al. Generation of Human Embryonic Stem Cell-Derived Lung Organoids for Modeling Infection and Replication Differences between Human Adenovirus Types 3 and 55 and Evaluating Potential Antiviral Drugs. J Virol 97:e0020923 (2023). PubMed: 37120831
- Blackstone BN et al. Laser Micropatterning Promotes Rete Ridge Formation and Enhanced Engineered Skin Strength without Increased Inflammation. Bioengineering (Basel) 10:N/A (2023). PubMed: 37508888
- Niethamer TK et al. Atf3 defines a population of pulmonary endothelial cells essential for lung regeneration. Elife 12:N/A (2023). PubMed: 37233732