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AB173285

重组Anti-PNUTS抗体[EPR11706]

Anti-PNUTS antibody [EPR11706]

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(2 Publications)

Rabbit Recombinant Monoclonal PNUTS antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications.

查看别名

CAT53, FB19, PNUTS, PPP1R10, Serine/threonine-protein phosphatase 1 regulatory subunit 10, MHC class I region proline-rich protein CAT53, PP1-binding protein of 114 kDa, Phosphatase 1 nuclear targeting subunit, Protein FB19, p99

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNUTS antibody [EPR11706] (AB173285)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNUTS antibody [EPR11706] (AB173285)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling PNUTS with ab173285 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-PNUTS antibody [EPR11706] (AB173285)
  • WB

Supplier Data

Western blot - Anti-PNUTS antibody [EPR11706] (AB173285)

All lanes:

Western blot - Anti-PNUTS antibody [EPR11706] (ab173285) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

Human fetal brain tissue lysate at 10 µg

Lane 3:

HepG2 cell lysate at 10 µg

Lane 4:

Jurkat cell lysate at 10 µg

Predicted band size: 99 kDa

false

Immunocytochemistry/ Immunofluorescence - Anti-PNUTS antibody [EPR11706] (AB173285)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PNUTS antibody [EPR11706] (AB173285)

Immunofluorescent analysis of HeLa cells labeling PNUTS with ab173285 at 1/100 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNUTS antibody [EPR11706] (AB173285)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNUTS antibody [EPR11706] (AB173285)

Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling PNUTS with ab173285 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR11706

亚型

IgG

不含载体蛋白

No

反应种属

Human

应用

ICC/IF, WB, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>" } } }
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产品详情

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The PNUTS protein also known as 'little PNUTS' acts as a regulatory subunit in the cell. It plays a role in controlling the activity of protein phosphatase 1 (PP1) essential for various cellular processes. The PNUTS protein has a molecular mass of approximately 106 kDa. It is expressed in multiple tissues with significant presence in the brain and heart suggesting its importance in these areas.
Biological function summary

The PNUTS protein influences many cellular events as it is part of a larger complex. It controls the dephosphorylation activity of PP1 impacting processes like cell cycle regulation and chromatin structure modification. This protein contributes to the dynamic regulation of transcription DNA repair and cell survival. Its ability to interact with other proteins positions it as a versatile component in cell biology.

Pathways

The PNUTS protein interacts in several notable pathways that affect cell function. It is involved in the DNA damage response pathway where it regulates DNA repair through its interaction with components like PP1. It also plays a role in the Rb signaling pathway related to cell cycle progression linking its function to cell growth and division. Through these pathways PNUTS connects with other proteins emphasizing its versatility.

Alterations in the PNUTS protein correlate with various conditions. Its dysfunction is associated with cancer impacting cell proliferation through its role in the Rb pathway. The connection between PNUTS and neurodegenerative disorders also emerges due to its high expression in the brain. In diseases like Alzheimer's PNUTS interactions with proteins like tau influence disease progression making it a target of interest in therapeutic research.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Scaffold protein which mediates the formation of the PTW/PP1 phosphatase complex by providing a binding platform to each component of the complex. The PTW/PP1 phosphatase complex plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase. Mediates interaction of WDR82 and PPP1CA. Inhibitor of PPP1CA and PPP1CC phosphatase activities. Has inhibitory activity on PPP1CA only when phosphorylated. Binds to mRNA, single-stranded DNA (ssDNA), poly(A) and poly(G) homopolymers (By similarity).
See full target information PPP1R10
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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Nature cell biology 19:1443 PubMed29184176

2017

Addendum: A regulated PNUTS mRNA to lncRNA splice switch mediates EMT and tumour progression.

Applications

Unspecified application

Species

Unspecified reactive species

Simon Grelet,Laura A Link,Breege Howley,Clémence Obellianne,Viswanathan Palanisamy,Vamsi K Gangaraju,J Alan Diehl,Philip H Howe

eNeuro 4: PubMed28955722

2017

The Progestin Receptor Interactome in the Female Mouse Hypothalamus: Interactions with Synaptic Proteins Are Isoform Specific and Ligand Dependent.

Applications

Unspecified application

Species

Unspecified reactive species

Kalpana D Acharya,Sabin A Nettles,Katherine J Sellers,Dana D Im,Moriah Harling,Cassandra Pattanayak,Didem Vardar-Ulu,Cheryl F Lichti,Shixia Huang,Dean P Edwards,Deepak P Srivastava,Larry Denner,Marc J Tetel
View all publications
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