Anti-PNUTS抗体(ab70247)
Key features and details
- Rabbit polyclonal to PNUTS
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-PNUTS抗体
参阅全部 PNUTS 一抗 -
描述
兔多克隆抗体to PNUTS -
宿主
Rabbit -
特异性
We have data to indicate that this antibody may not cross react with Xenopus laevis. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues. -
经测试应用
适用于: WB, IP, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
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免疫原
Synthetic peptide corresponding to PNUTS aa 350-450.
Database link: Q96QC0 -
阳性对照
- Nuclear extract from Hela cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Antibody was affinity purified using an epitope specific to PNUTS immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab70247于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 48, 115 kDa (predicted molecular weight: 99 kDa).
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| IP |
Use a concentration of 1 - 10 µg/ml.
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| IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 说明 |
|---|
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WB
1/1000 - 1/10000. Detects a band of approximately 48, 115 kDa (predicted molecular weight: 99 kDa). |
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IP
Use a concentration of 1 - 10 µg/ml. |
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IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Scaffold protein which mediates the formation of the PTW/PP1 phosphatase complex by providing a binding platform to each component of the complex. The PTW/PP1 phosphatase complex plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase. Mediates interaction of WDR82 and PPP1CA. Inhibitor of PPP1CA and PPP1CC phosphatase activities. Has inhibitory activity on PPP1CA only when phosphorylated. Binds to mRNA, single-stranded DNA (ssDNA), poly(A) and poly(G) homopolymers. -
组织特异性
Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. -
序列相似性
Contains 1 C3H1-type zinc finger.
Contains 1 TFIIS N-terminal domain. -
翻译后修饰
Phosphorylated on Ser-398 by PKA within the region necessary for interaction with PPP1CA. -
细胞定位
Nucleus. Found in discrete nucleoplasmic bodies and within nucleoli. Associates with chromatin during interphase, excluded from condensed chromosomes during early mitosis and is reloaded onto chromosomes at the late telophase (By similarity). - Information by UniProt
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数据库链接
- Entrez Gene: 462544 Chimpanzee
- Entrez Gene: 5514 Human
- Entrez Gene: 52040 Mouse
- Entrez Gene: 711949 Rhesus monkey
- Omim: 603771 Human
- SwissProt: Q7YR38 Chimpanzee
- SwissProt: Q96QC0 Human
- SwissProt: Q80W00 Mouse
see all -
别名
- CAT 53 antibody
- CAT53 antibody
- FB 19 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNUTS antibody (ab70247)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling PNUTS with ab70247 at 1/1000 (1µg/ml). Detection: DAB.
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Western blot - Anti-PNUTS antibody (ab70247)Lanes 1-2 : Anti-PNUTS antibody (ab70247) at 1 µg/ml
Lanes 3-4 : Anti-PNUTS antibody (ab70247) at 0.2 µg/ml
Lanes 1 & 3 : Nuclear extract from Hela cells at 15 µg
Lanes 2 & 4 : Nuclear extract from Hela cells at 45 µg
Predicted band size: 99 kDa
Observed band size: 115 kDa why is the actual band size different from the predicted?
Additional bands at: 48 kDa. We are unsure as to the identity of these extra bands. -
Immunoprecipitation - Anti-PNUTS antibody (ab70247)Immunoprecipitation/ Western Blot of PNUTS.
Sample: Nuclear extract from Hela cells lysate at 100µg for IP.
Lane 1: Another antibody which recognizes a different epitope of PNUTS.
Lane 2: Another antibody which recognizes a different epitope of PNUTS.
Lane 3: ab70247 at 1µg/100µg nuclear extract.
Lane 4: Another antibody which recognizes a different epitope of PNUTS.
Detection: Chemiluminescence with exposure times of 3 seconds.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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