Anti-PNPLA8抗体(ab223726)
Key features and details
- Rabbit polyclonal to PNPLA8
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-PNPLA8抗体
参阅全部 PNPLA8 一抗 -
描述
兔多克隆抗体to PNPLA8 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment corresponding to Human PNPLA8 aa 100-250.
Database link: Q9NP80 -
阳性对照
- WB: U-2197 cell lysate. ICC/IF: U-2 OS cells. HC-P: Human duodenum tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab223726于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 88 kDa.
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| IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
| 说明 |
|---|
|
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 88 kDa. |
|
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
靶标
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功能
Calcium-independent phospholipase A2, which catalyzes the hydrolysis of the sn-2 position of glycerophospholipids, PtdSer and to a lower extent PtdCho. Cleaves membrane phospholipids. -
组织特异性
Expressed in parenchymal tissues including heart, skeletal muscle, placenta, brain, liver and pancreas. Also expressed in bronchial epithelial cells and kidney. Highest expression is observed in skeletal muscle and heart. -
序列相似性
Contains 1 patatin domain. -
细胞定位
Endoplasmic reticulum membrane. Golgi apparatus membrane. Cytoplasm > perinuclear region. - Information by UniProt
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数据库链接
- Entrez Gene: 50640 Human
- Omim: 612123 Human
- SwissProt: Q9NP80 Human
- Unigene: 617340 Human
- Unigene: 732217 Human
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别名
- Calcium-independent phospholipase A2-gamma antibody
- Intracellular membrane associated calcium independent phospholipase A2 gamma antibody
- Intracellular membrane-associated calcium-independent phospholipase A2 gamma antibody
see all
图片
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Western blot - Anti-PNPLA8 antibody (ab223726)Anti-PNPLA8 antibody (ab223726) at 1/100 dilution + U-2197 cell lysate
Predicted band size: 88 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-PNPLA8 antibody (ab223726)PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for PNPLA8 (green) using ab223726 (4 µg/ml) in ICC/IF.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNPLA8 antibody (ab223726)Paraffin embedded human duodenum tissue stained for PNPLA8 with ab223726 (1/50 dilution) in immunohistochemical analysis.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab223726 尚未被引用在任何文献中。