AB167427

重组Anti-PNMT抗体[EPR7547(2)]

Anti-PNMT antibody [EPR7547(2)]

RabMAb
Recombinant
了解详情

(1 Review)

(5 Publications)

Rabbit Recombinant Monoclonal PNMT antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 5 publications.

查看别名

PENT, PNMT, Phenylethanolamine N-methyltransferase, PNMTase, Noradrenaline N-methyltransferase

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-PNMT antibody [EPR7547(2)] (AB167427)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PNMT antibody [EPR7547(2)] (AB167427)

Immunofluorescence analysis of K562 cells, labeling PNMT using ab167427 at a 1/50 dilution.

Unknown

Western blot - Anti-PNMT antibody [EPR7547(2)] (AB167427)

All lanes:

Western blot - Anti-PNMT antibody [EPR7547(2)] (ab167427) at 1/1000 dilution

Lane 1:

K562 lysate at 10 µg

Lane 2:

Human adrenal gland lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 31 kDa

false

Supplier Data

Western blot - Anti-PNMT antibody [EPR7547(2)] (AB167427)

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-PNMT antibody [EPR7547(2)] (ab167427) at 1/1000 dilution

All lanes:

Mouse kidney tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 31 kDa,85 kDa

Observed band size: 31 kDa

false

Exposure time: 3min

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR7547(2)

亚型

IgG

不含载体蛋白

反应种属

Mouse, Human

应用

WB, ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

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性能和储存信息

形式

Liquid

纯度

Tissue culture supernatant

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The protein PNMT also known as Phenylethanolamine N-methyltransferase functions as an enzyme responsible for the methylation of norepinephrine to synthesize epinephrine. It belongs to the methyltransferase family and weighs approximately 30 kDa. PNMT is highly expressed in the adrenal medulla particularly in chromaffin cells but also found in smaller amounts in some parts of the brain such as the medulla oblongata and retina. This distribution supports its role in catecholamine biosynthesis and regulation.

Biological function summary

The conversion of norepinephrine to epinephrine increases the functionality of PNMT in stress response mechanisms. PNMT plays an important role in the synthesis of catecholamines which are essential for modulating the fight-or-flight response. It does not form part of a larger protein complex but interacts with substrates and cofactors directly to catalyze its reactions. PNMT's activity is carefully regulated by factors including substrate availability and enzyme expression levels in tissues where it operates.

Pathways

Norepinephrine to epinephrine synthesis conducted by PNMT marks the final step in the catecholamine biosynthetic pathway. This pathway is critical in managing cardiovascular and metabolic functions. The protein connects extensively with enzymes such as tyrosine hydroxylase and dopamine β-hydroxylase within this pathway which are involved in prior steps of catecholamine synthesis. PNMT's activity and regulation are therefore essential for maintaining homeostasis in stress and energy metabolism.

PNMT connects directly to conditions like hypertension and certain phaeochromocytomas which arise from catecholamine excess or imbalance. Elevated levels of PNMT can be found in tumors linked to the adrenal medulla affecting the production of epinephrine. PNMT's interaction with enzymes like dopamine β-hydroxylase is relevant in understanding these disorders since imbalances in enzyme activity can lead to disrupted catecholamine synthesis contributing to disease pathogenesis.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Catalyzes the transmethylation of nonepinephrine (noradrenaline) to form epinephrine (adrenaline), using S-adenosyl-L-methionine as the methyl donor (PubMed : 20496117). Other substrates include phenylethanolamine and octopamine (PubMed : 16277617, PubMed : 16363801, PubMed : 8812853). Also methylates normetanephrine (By similarity).

See full target information PNMT

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文献 (5)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine reports 27: PubMed36601769

2023

Nerve growth factor causes epinephrine release dysfunction by regulating phenotype alterations and the function of adrenal medullary chromaffin cells in mice with allergic rhinitis.

Applications

Unspecified application

Species

Unspecified reactive species

Chao Liu,Jian-Ping Liang,Xiao-Lin Huang,Zhong Liu,An-Bing Zhang,Nian-Gen Deng,Zi-Feng Wei,Jun Wang

Oncotarget 12:49-60 PubMed33456713

2021

The noradrenergic profile of plasma metanephrine in neuroblastoma patients is reproduced in xenograft mice models and arise from PNMT downregulation.

Applications

Unspecified application

Species

Unspecified reactive species

Karim Abid,Maja Beck Popovic,Katia Balmas Bourloud,Jacqueline Schoumans,Joana Grand-Guillaume,Eric Grouzmann,Annick Mühlethaler-Mottet

American journal of physiology. Regulatory, integr 317:R407-R417 PubMed31242021

2019

Regulation of catecholamine release from the adrenal medulla is altered in deer mice () native to high altitudes.

Applications

Unspecified application

Species

Unspecified reactive species

Angela L Scott,Nicole A Pranckevicius,Colin A Nurse,Graham R Scott

Scientific data 4:170134 PubMed28949324

2017

Three-dimensional image reconstruction of distribution of Pnmt cell-derived cells in murine heart.

Applications

IHC-Fr

Species

Mouse

Haibo Ni,Yange Wang,William Crawford,Shanzhuo Zhang,Longxian Cheng,Henggui Zhang,Ming Lei

The Journal of endocrinology 230:309-23 PubMed27402067

2016

Effect of heart failure on catecholamine granule morphology and storage in chromaffin cells.

Applications

Unspecified application

Species

Unspecified reactive species

Sushil K Mahata,Hong Zheng,Sumana Mahata,Xuefei Liu,Kaushik P Patel

View all publications

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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

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For licensing inquiries, please contact partnerships@abcam.com

重组Anti-PNMT抗体[EPR7547(2)]

Anti-PNMT antibody [EPR7547(2)]

Immunocytochemistry/ Immunofluorescence - Anti-PNMT antibody [EPR7547(2)] (AB167427)

Western blot - Anti-PNMT antibody [EPR7547(2)] (AB167427)

Western blot - Anti-PNMT antibody [EPR7547(2)] (AB167427)

关键信息

宿主种属

克隆

克隆号

亚型

不含载体蛋白

反应种属

应用

免疫原