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重组RabMAb

重组Anti-PNAD抗体[EPR28215-189] - BSA and Azide free (ab318271)

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Western blot - Anti-PNAD antibody [EPR28215-189] - BSA and Azide free (ab318271)
  • Western blot - Anti-PNAD antibody [EPR28215-189] - BSA and Azide free (ab318271)
  • Anti-PNAD antibody [EPR28215-189] - BSA and Azide free (ab318271)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR28215-189] to PNAD - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Unconjugated

概述

  • 产品名称

    Anti-PNAD抗体[EPR28215-189] - BSA and Azide free
    参阅全部 PNAD 一抗

  • 描述

    兔单克隆抗体[EPR28215-189] to PNAD - BSA and Azide free

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WBmore details
    不适用于: Flow Cyt (Intra),ICC/IF,IHC-P or IP

  • 种属反应性

    与反应: Human
    不与反应: Mouse, Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: 293T transfected with scrambled siRNA, 293T transfected with siRNA specifically targeting PNAD, HepG2, Human colon tissue and SH-SY5Y lysates.

  • 常规说明

    ab318271 is the carrier-free version of ab318270

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab318271于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
Use at an assay dependent concentration.
说明
WB
Use at an assay dependent concentration.
应用说明
Is unsuitable for Flow Cyt (Intra),ICC/IF,IHC-P or IP.

靶标

  • 相关性

    PNAD is required for the ubiquitin-dependent turnover of intracellular proteins that initiate with Met-Asn. This enzyme does not act on substrates with internal or C-terminal asparagines and does not act on glutamine residues in any position.

  • 细胞定位

    Cytoplasmic

  • 数据库链接

    • 别名

      • N terminal Asn amidase antibody
      • N terminal asparagine amidase antibody
      • N terminal asparagine amidohydrolase antibody
      • NTAN1 antibody
      • NTN-amidase antibody
      • PNAA antibody
      • PNAD antibody
      • Protein N-terminal Asn amidase antibody
      • Protein N-terminal asparagine amidase antibody
      • Protein N-terminal asparagine amidohydrolase antibody
      • Protein NH2-terminal asparagine amidohydrolase antibody
      • Protein NH2-terminal asparagine deamidase antibody
      • Protein NTN-amidase antibody
      see all

    图片

    • Western blot - Anti-PNAD antibody [EPR28215-189] - BSA and Azide free (ab318271)
      Western blot - Anti-PNAD antibody [EPR28215-189] - BSA and Azide free (ab318271)
      All lanes : Anti-PNAD antibody [EPR28215-189] (ab318270) at 1/1000 dilution

      Lane 1 : 293T (human embryonic kidney epithelial cell) transfected with scrambled siRNA control whole cell lysate
      Lane 2 : 293T transfected with siRNA specifically targeting PNAD whole cell lysate
      Lane 3 : HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Observed band size: 34 kDa why is the actual band size different from the predicted?



      This data was developed using ab318270, the same antibody clone in a different buffer formulation.

      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      Lane1 and 2 were freshly made and used for Western Blotting immediately to minimize protein degradation.

      Lane1 and 2 were developed using a higher sensitivity ECL substrate.

      In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control  (ab181602) staining at 1/200000 dilution.

      Exposure time: Lane1-2: 136 seconds, lane3: 180 seconds

    • Western blot - Anti-PNAD antibody [EPR28215-189] - BSA and Azide free (ab318271)
      Western blot - Anti-PNAD antibody [EPR28215-189] - BSA and Azide free (ab318271)
      All lanes : Anti-PNAD antibody [EPR28215-189] (ab318270) at 1/1000 dilution

      Lane 1 : Human colon tissue lysate
      Lane 2 : SH-SY5Y (human neuroblastoma epithelial cell) whole fresh cell lysate

      Lysates/proteins at 50 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG (Merck DC03L) at 1/5000 dilution

      Observed band size: 34 kDa why is the actual band size different from the predicted?


      Exposure time: 180 seconds


      This data was developed using ab318270, the same antibody clone in a different buffer formulation.

      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      The identity of the lower MW band at approximately 20 kDa is unknown.

      In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control  (ab181602) staining at 1/200000 dilution.

    • Anti-PNAD antibody [EPR28215-189] - BSA and Azide free (ab318271)
      Anti-PNAD antibody [EPR28215-189] - BSA and Azide free (ab318271)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

    发表研究结果有使用 ab318271？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab318271 尚未被引用在任何文献中。

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