重组Anti-PMCA1抗体[EPR12029] (ab190355)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12029] to PMCA1
- Suitable for: IP, WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-PMCA1抗体[EPR12029]
参阅全部 PMCA1 一抗 -
描述
兔单克隆抗体[EPR12029] to PMCA1 -
宿主
Rabbit -
经测试应用
适用于: IP, WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: THP-1, HeLa, Neuro-2a, U87-MG, HepG2, C6 cell lysates. IHC-P: Human stomach and Human transitional cell carcinoma of bladder tissues, mouse lung, mouse stomach, rat stomach.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR12029 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
- Anti-PMCA1 antibody [EPR12029] - BSA and Azide free (ab251018)
- APC Anti-PMCA1 antibody [EPR12029] (ab319206)
- PE Anti-PMCA1 antibody [EPR12029] (ab319354)
- Alexa Fluor® 488 Anti-PMCA1 antibody [EPR12029] (ab319488)
- Alexa Fluor® 647 Anti-PMCA1 antibody [EPR12029] (ab319601)
- Alexa Fluor® 594 Anti-PMCA1 antibody [EPR12029] (ab319705)
- Alexa Fluor® 750 Anti-PMCA1 antibody [EPR12029] (ab321313)
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab190355于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IP |
1/1000.
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WB |
1/1000 - 1/10000. Detects a band of approximately 130-250 kDa (predicted molecular weight: 139 kDa).
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IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
说明 |
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IP
1/1000. |
WB
1/1000 - 1/10000. Detects a band of approximately 130-250 kDa (predicted molecular weight: 139 kDa). |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
靶标
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功能
This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium out of the cell. -
组织特异性
Isoform B is ubiquitously expressed. Isoform C is found in brain cortex, skeletal muscle and heart muscle. Isoform D has only been found in fetal skeletal muscle. Isoform K has been found in small intestine and liver. -
序列相似性
Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIB subfamily. -
结构域
The calmodulin-binding subdomain B is different in the different splice variants and shows pH dependent calmodulin binding properties in isoforms A, C, D and E. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 490 Human
- Entrez Gene: 67972 Mouse
- Entrez Gene: 29598 Rat
- Omim: 108731 Human
- SwissProt: P20020 Human
- SwissProt: P11505 Rat
- Unigene: 506276 Human
- Unigene: 7208 Rat
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别名
- AT2B1_HUMAN antibody
- ATP2B1 antibody
- ATPase Ca++ transporting plasma membrane 1 antibody
see all
图片
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All lanes : Anti-PMCA1 antibody [EPR12029] (ab190355) at 1/5000 dilution
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate, boiled
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate, unboiled
Lane 3 : Neuro-2a (Mouse neuroblastoma neuroblast) whole cell lysate, boiled
Lane 4 : Neuro-2a (Mouse neuroblastoma neuroblast) whole cell lysate, unboiled
Lane 5 : C6 (Rat glial tumor glial cell) whole cell lysate, boiled
Lane 6 : C6 (Rat glial tumor glial cell) whole cell lysate, unboiled
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 139 kDa
Observed band size: 130-250 kDa why is the actual band size different from the predicted?Blocking/Diluting buffer and concentration: 5% NFDM/TBST.
Exposure time:
Lanes 1, 2: 7 seconds
Lanes 3, 4, 5, 6: 3 secondsab181602 is used as a Loading Control at dilution 1/1000000.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMCA1 antibody [EPR12029] (ab190355)
Immunohistochemical analysis of paraffin-embedded rat stomach tissue labelling PMCA1 with ab190355 at 1/2000 dilution, followed by ready-to-use secondary antibody Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) at dilution 1/1. Counterstained with Hematoxylin.
Performed heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Positive staining mouse lung cancer.
The section was incubated with ab190355 for 10 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.
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All lanes : Anti-PMCA1 antibody [EPR12029] (ab190355) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : ATP2B1 knockout HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : THP-1 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 139 kDa
Observed band size: 180-245 kDa why is the actual band size different from the predicted?Lanes 1-4: Merged signal (red and green). Green - ab190355 observed at 180-245 kDa. Red - loading control ab8245 observed at 36 kDa.
ab190355 Anti-PMCA1 antibody [EPR12029] was shown to specifically react with PMCA1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265561 (knockout cell lysate ab257365) was used. Wild-type and PMCA1 knockout samples were subjected to SDS-PAGE. ab190355 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2. 5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMCA1 antibody [EPR12029] (ab190355)
Immunohistochemical analysis of paraffin-embedded mouse lung cancer tissue labelling PMCA1 with ab190355 at 1/2000 dilution, followed by ready-to-use secondary antibody Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) at dilution 1/1. Counterstained with Hematoxylin.
Performed heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Positive staining mouse lung cancer.
The section was incubated with ab190355 for 10 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.
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All lanes : Anti-PMCA1 antibody [EPR12029] (ab190355) at 1/2000 dilution
Lane 1 : U87-MG cell lysate
Lane 2 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 139 kDa
Observed band size: 130-250 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMCA1 antibody [EPR12029] (ab190355)
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue labelling PMCA1 with ab190355 at 1/2000 dilution, followed by ready-to-use secondary antibody Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) at dilution 1/1. Counterstained with Hematoxylin.
Performed heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Positive staining mouse stomach.
The section was incubated with ab190355 for 10 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.
-
All lanes : Anti-PMCA1 antibody [EPR12029] (ab190355) at 1/2000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : Neuro-2a cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 139 kDa
Observed band size: 130-250 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMCA1 antibody [EPR12029] (ab190355)
Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling PMCA1 with ab190355 at 1/500 dilution. A prediluted HRP Polymer for Rabbit IgG as secondary antibody. Counterstained with Hematoxylin. Negative control also shown.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMCA1 antibody [EPR12029] (ab190355)
Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma of bladder tissue labeling PMCA1 with ab190355 at 1/500 dilution. A prediluted HRP Polymer for Rabbit IgG as secondary antibody. Counterstained with Hematoxylin. Negative control also shown.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (13)
ab190355 被引用在 13 文献中.
- Kondolf HC et al. Protein engineering reveals that gasdermin A preferentially targets mitochondrial membranes over the plasma membrane during pyroptosis. J Biol Chem 299:102908 (2023). PubMed: 36642180
- Suntornsaratoon P et al. Running exercise with and without calcium supplementation from tuna bone reduced bone impairment caused by low calcium intake in young adult rats. Sci Rep 13:9568 (2023). PubMed: 37311761
- Zhang Z et al. Increasing serotonin concentrations alter calcium metabolism in periparturient dairy goats. J Anim Sci 100:N/A (2022). PubMed: 35235945
- Rodrat M et al. Vasoactive intestinal peptide and cystic fibrosis transmembrane conductance regulator contribute to the transepithelial calcium transport across intestinal epithelium-like Caco-2 monolayer. PLoS One 17:e0277096 (2022). PubMed: 36399482
- Ilic K et al. Plasma Membrane Calcium ATPase-Neuroplastin Complexes Are Selectively Stabilized in GM1-Containing Lipid Rafts. Int J Mol Sci 22:N/A (2021). PubMed: 34948386