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Signal Transduction Signaling Pathway Calcium Signaling Calcium Channels

Anti-PMCA1抗体(ab3528)

  • Datasheet
Reviews (3)Q&A (2)References (12)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMCA1 antibody (ab3528)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMCA1 antibody (ab3528)

Key features and details

  • Rabbit polyclonal to PMCA1
  • Suitable for: ELISA, ICC, IHC-Fr, ICC/IF, WB, IP, IHC-P
  • Reacts with: Mouse, Rat, Human, Pig, Non human primates
  • Isotype: IgG

选择批间可重复性更高的重组抗体

Product image
Anti-PMCA1 antibody [EPR12029] (ab190355)
  • 研究可靠 —— 各批次间结果一致且可重复
  • 长期批量供应 —— 采用重组技术,可实现快速生产
  • 首次实验即可成功 —— 经过大量验证确认了特异性
  • 符合伦理标准 —— 产品不含动物成分

概述

  • 产品名称

    Anti-PMCA1抗体
    参阅全部 PMCA1 一抗
  • 描述

    兔多克隆抗体to PMCA1
  • 宿主

    Rabbit
  • 特异性

    Detects PMCA 1a and 1b ATPase.
  • 经测试应用

    适用于: ELISA, ICC, IHC-Fr, ICC/IF, WB, IP, IHC-Pmore details
  • 种属反应性

    与反应: Mouse, Rat, Human, Pig, Non human primates
  • 免疫原

    Synthetic peptide corresponding to Rat PMCA1 aa 1-100.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 阳性对照

    • IHC-P: Human skeletal muscle and brain tissue.
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Signal Transduction
    • Signaling Pathway
    • Calcium Signaling
    • Calcium Channels
    • Signal Transduction
    • Metabolism
    • Plasma Membrane
    • ATPases
    • Metabolism
    • Types of disease
    • Cancer

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab3528于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ELISA
Use at an assay dependent concentration.
ICC
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration. PubMed: 16803870
WB (2)
Use at an assay dependent concentration. Predicted molecular weight: 139 kDa.

This antibody detects ~134 kDa and ~130 kDa proteins representing PMCA 1a and 1b ATPase from rat samples.

IP
Use at an assay dependent concentration.
IHC-P (1)
1/20.
说明
ELISA
Use at an assay dependent concentration.
ICC
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration. PubMed: 16803870
WB
Use at an assay dependent concentration. Predicted molecular weight: 139 kDa.

This antibody detects ~134 kDa and ~130 kDa proteins representing PMCA 1a and 1b ATPase from rat samples.

IP
Use at an assay dependent concentration.
IHC-P
1/20.

靶标

  • 功能

    This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium out of the cell.
  • 组织特异性

    Isoform B is ubiquitously expressed. Isoform C is found in brain cortex, skeletal muscle and heart muscle. Isoform D has only been found in fetal skeletal muscle. Isoform K has been found in small intestine and liver.
  • 序列相似性

    Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIB subfamily.
  • 结构域

    The calmodulin-binding subdomain B is different in the different splice variants and shows pH dependent calmodulin binding properties in isoforms A, C, D and E.
  • 细胞定位

    Cell membrane.
  • Target information above from: UniProt accession P20020 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 490 Human
    • Entrez Gene: 67972 Mouse
    • Entrez Gene: 397636 Pig
    • Entrez Gene: 29598 Rat
    • Omim: 108731 Human
    • SwissProt: P20020 Human
    • SwissProt: P23220 Pig
    • SwissProt: P11505 Rat
    • Unigene: 506276 Human
    • Unigene: 7208 Rat
    see all
  • 别名

    • AT2B1_HUMAN antibody
    • ATP2B1 antibody
    • ATPase Ca++ transporting plasma membrane 1 antibody
    • Plasma membrane calcium ATPase 1 antibody
    • Plasma membrane calcium ATPase isoform 1 antibody
    • Plasma membrane calcium pump isoform 1 antibody
    • Plasma membrane calcium transporting ATPase 1 antibody
    • Plasma membrane calcium-transporting ATPase 1 antibody
    • PMCA 1 antibody
    • PMCA1 antibody
    see all

图片

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMCA1 antibody (ab3528)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMCA1 antibody (ab3528)
    Immunohistochemistry was performed on normal biopsies of deparaffinized Human skeletal muscle tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a rabbit polyclonal antibody recognizing PMCA1 ATPase ab3528 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMCA1 antibody (ab3528)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMCA1 antibody (ab3528)
    Immunohistochemistry was performed on normal biopsies of deparaffinized Human brain tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a rabbit polyclonal antibody recognizing PMCA1 ATPase ab3528 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

实验方案

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

数据表及文件

  • Datasheet download

    Download

文献 (12)

发表研究结果有使用 ab3528?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab3528 被引用在 12 文献中.

  • Chan CSY  et al. Cell type- and stage-specific expression of Otx2 is regulated by multiple transcription factors and cis-regulatory modules in the retina. Development 147:N/A (2020). PubMed: 32631829
  • Lau MT  et al. Molecular dissection of box jellyfish venom cytotoxicity highlights an effective venom antidote. Nat Commun 10:1655 (2019). PubMed: 31040274
  • Schmidt N  et al. Neuroplastin and Basigin Are Essential Auxiliary Subunits of Plasma Membrane Ca2+-ATPases and Key Regulators of Ca2+ Clearance. Neuron 96:827-838.e9 (2017). PubMed: 29056295
  • Wakui H  et al. Enhancement of intrarenal plasma membrane calcium pump isoform 1 expression in chronic angiotensin II-infused mice. Physiol Rep 5:N/A (2017). WB . PubMed: 28611155
  • Robertson SYT  et al. Multiple Calcium Export Exchangers and Pumps Are a Prominent Feature of Enamel Organ Cells. Front Physiol 8:336 (2017). IF . PubMed: 28588505
View all Publications for this product

客户评价及客户问答

Show All 评价 Q&A
提交评价 提交问题

1-5 of 5 Abreviews or Q&A

Western blot abreview for Anti-PMCA1 antibody

Excellent
Abreviews
Abreviews
Application
Western blot
Sample
Rat Cell lysate - whole cell (neonatal rat cardiomyocytes)
Loading amount
50 µg
Specification
neonatal rat cardiomyocytes
Gel Running Conditions
Reduced Denaturing (%8 tris HCL)
Blocking step
BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jul 28 2011

Western blot abreview for Anti-PMCA1 antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Rat Tissue lysate - whole (Rat hippocampal slice and slice culture)
Loading amount
25 µg
Specification
Rat hippocampal slice and slice culture
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Dr. R Empson

Verified customer

提交于 Aug 14 2007

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PMCA1 antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Rat Brain Hippocampal Slices and Slice Cultures)
Specification
Rat Brain Hippocampal Slices and Slice Cultures
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
Yes - 1% Triton X-100
Blocking step
BSA as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 37°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Dr. R Empson

Verified customer

提交于 Aug 09 2007

Question

Hi
The details of the procedure used are below,
1. When performing the ICC experiment, was any blocking used, if so
what? Also, was any permiabilisation performed?
Blocking/permiabilisation buffer: 0.1% Triton X-100 and 10% Horse serum
in HBSS.
2. When performing the Western blot with the antibody, could you
describe how the samples were prepared? What lysis buffer was used,
were any protease inhibitors used? Also, at what dilution was the
primary antibody used in?
Lysis buffer contains spermidine, inhibitors (pH 7.9). Sample boiled
for 10 min in SDS
Gel: 10% SDS
Protein loaded: 70 ug per lane
Blocking: 5% BSA 1 hr Room Temperature
1 Ab: 1:1000 dilution 4 degree overnight
3. Have the secondary antibodies used been used with any other
primary antibodies previously with no problem?
2 ab: anti Rabbit Jacksons 1 hr (I routinely use this with other
primary antibodies with excellent outcome)
chemiluminescence detection with ECL millipore
4. Do you have the lot number used (or the order, or the
approximate date of the order and the delivery address)?

Read More

Abcam community

Verified customer

Asked on Nov 20 2012

Answer

Thank you for taking time to provide me with that extra information. It has been very helpful.

The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality.

Having reviewed this case, I would like to offer some suggestions to help optimize the results from ab3528.

1. As the target it a membrane bound protein I would suggest using a milder detergent for permiabilisation as Triton X may be too harsh. A detergent such as Tween 20, Saponin or Digitonin may be preferential as these give large enough pores for antibodies to go through without dissolving plasma membrane. It may also be worth trying a different blocking agent, such as 5% milk or BSA.

2. With the Western blot, I would suggest reducing the amount of protein currently loaded to 20 ug. I would also suggest reducing the heating step to 10 minutes at 70°C as this can reduce aggregation, especially when targeting membrane proteins such as PMCA1. I think it would also be worthwhile trying an alternative lysis buffer such as RIPA in order to make sure the protein is well solubilised. As with the ICC experiment, I would also try a different blocking buffer to see if the results are affected, such as 5% milk. This can have a surprisingly stark effect with some antibodies, as illustrated with the anti-GAPDH ab9385:

https://www.abcam.com/ab9385

Should the suggestions not improve the results, please do let me know.

In the event that a product is not functioning in the species and applications cited on the product datasheet (and the problem has been reported within 6 months of purchase), we would be pleased to provide a free of charge replacement, credit note, or refund.

I hope this information is helpful, and I thank you for your cooperation.

Read More

Abcam Scientific Support

回复于 Nov 20 2012

Question

Hi,
We have tried
using the antibody against PMCA1 (ab 3528) and have not been able to
see expected results in Western Blots and Immunoflurescence detection.
Attached here are the details:
ICC
sample: rat hippocampal neurons 16 DIV
Fixation: 4% PFA , 8 minutes, RT
1° Ab: 1:200, 2 hours, RT
2° Ab: 1:1000 Cy3-conjugated, 1 hour, RT
No signal
Western Blot
sample: mouse brain membrane fraction
protein per lane: 50ug
2°Ab: anti rabbit
blocking: 5% BSA 1 hr
1°Ab: 4°C overnight
Chemiluminescence: ECL
Result blot attached
We would highly appreciate help from the technical support.

Read More

Abcam community

Verified customer

Asked on Oct 26 2012

Answer

Thank you for contacting us and for sharing the problems you have been experiencing with the Anti-PMCA1 antibody (ab3528). I am sorry to hear you are experiencing difficulties with this product. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality.

Having reviewed this case, I have a few further questions if you wouldn't mind. This should allow me to more fully understand what may be contributing to these problems.

1. When performing the ICC experiment, was any blocking used, if so what? Also, was any permiabilisation performed?

2. When performing the Western blot with the antibody, could you describe how the samples were prepared? What lysis buffer was used, were any protease inhibitors used? Also, at what dilution was the primary antibody used in?

3. Have the secondary antibodies used been used with any other primary antibodies previously with no problem?

4. Do you have the lot number used (or the order, or the approximate date of the order and the delivery address)?

With this information I hope to be able to understand more fully what may be contributing to these problems.

In the event that a product is not functioning in the species and applications cited on the product datasheet (and the problem has been reported within 6 months of purchase), we would be pleased to provide a free of charge replacement, credit note, or refund.

I hope this information is helpful, and I thank you for your cooperation.

Read More

Abcam Scientific Support

回复于 Oct 26 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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