Anti-PMCA1抗体(ab3528)
Key features and details
- Rabbit polyclonal to PMCA1
- Suitable for: ELISA, ICC, IHC-Fr, ICC/IF, WB, IP, IHC-P
- Reacts with: Mouse, Rat, Human, Pig, Non human primates
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-PMCA1抗体
参阅全部 PMCA1 一抗 -
描述
兔多克隆抗体to PMCA1 -
宿主
Rabbit -
特异性
Detects PMCA 1a and 1b ATPase. -
经测试应用
适用于: ELISA, ICC, IHC-Fr, ICC/IF, WB, IP, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Human, Pig, Non human primates -
免疫原
Synthetic peptide corresponding to Rat PMCA1 aa 1-100.
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阳性对照
- IHC-P: Human skeletal muscle and brain tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
应用 | Ab评论 | 说明 |
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ELISA |
Use at an assay dependent concentration.
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ICC |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration.
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ICC/IF |
Use at an assay dependent concentration. PubMed: 16803870
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WB | (2) |
Use at an assay dependent concentration. Predicted molecular weight: 139 kDa.
This antibody detects ~134 kDa and ~130 kDa proteins representing PMCA 1a and 1b ATPase from rat samples. |
IP |
Use at an assay dependent concentration.
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IHC-P | (1) |
1/20.
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说明 |
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ELISA
Use at an assay dependent concentration. |
ICC
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. PubMed: 16803870 |
WB
Use at an assay dependent concentration. Predicted molecular weight: 139 kDa. This antibody detects ~134 kDa and ~130 kDa proteins representing PMCA 1a and 1b ATPase from rat samples. |
IP
Use at an assay dependent concentration. |
IHC-P
1/20. |
靶标
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功能
This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium out of the cell. -
组织特异性
Isoform B is ubiquitously expressed. Isoform C is found in brain cortex, skeletal muscle and heart muscle. Isoform D has only been found in fetal skeletal muscle. Isoform K has been found in small intestine and liver. -
序列相似性
Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIB subfamily. -
结构域
The calmodulin-binding subdomain B is different in the different splice variants and shows pH dependent calmodulin binding properties in isoforms A, C, D and E. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 490 Human
- Entrez Gene: 67972 Mouse
- Entrez Gene: 397636 Pig
- Entrez Gene: 29598 Rat
- Omim: 108731 Human
- SwissProt: P20020 Human
- SwissProt: P23220 Pig
- SwissProt: P11505 Rat
see all -
别名
- AT2B1_HUMAN antibody
- ATP2B1 antibody
- ATPase Ca++ transporting plasma membrane 1 antibody
see all
图片
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Immunohistochemistry was performed on normal biopsies of deparaffinized Human skeletal muscle tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a rabbit polyclonal antibody recognizing PMCA1 ATPase ab3528 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
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Immunohistochemistry was performed on normal biopsies of deparaffinized Human brain tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a rabbit polyclonal antibody recognizing PMCA1 ATPase ab3528 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
实验方案
数据表及文件
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Datasheet download
文献 (12)
ab3528 被引用在 12 文献中.
- Chan CSY et al. Cell type- and stage-specific expression of Otx2 is regulated by multiple transcription factors and cis-regulatory modules in the retina. Development 147:N/A (2020). PubMed: 32631829
- Lau MT et al. Molecular dissection of box jellyfish venom cytotoxicity highlights an effective venom antidote. Nat Commun 10:1655 (2019). PubMed: 31040274
- Schmidt N et al. Neuroplastin and Basigin Are Essential Auxiliary Subunits of Plasma Membrane Ca2+-ATPases and Key Regulators of Ca2+ Clearance. Neuron 96:827-838.e9 (2017). PubMed: 29056295
- Wakui H et al. Enhancement of intrarenal plasma membrane calcium pump isoform 1 expression in chronic angiotensin II-infused mice. Physiol Rep 5:N/A (2017). WB . PubMed: 28611155
- Robertson SYT et al. Multiple Calcium Export Exchangers and Pumps Are a Prominent Feature of Enamel Organ Cells. Front Physiol 8:336 (2017). IF . PubMed: 28588505