AB81719

Anti-PLVAP/PV-1 抗体 [174/2]

Name: PLVAP/PV-1抗体[174/2] (ab81719)| Abcam中文官网
Brand: Abcam Limited
SKU: AB81719
Price: 4752 CNY
Availability: InStock
Rating: 3 (3 reviews)

Anti-PLVAP/PV-1 antibody [174/2]

(3 Reviews)

(14 Publications)

Mouse Monoclonal PLVAP/PV-1 antibody. Suitable for Flow Cyt, ICC/IF and reacts with Human samples. Cited in 14 publications.

查看别名

FELS, PV1, PLVAP, Plasmalemma vesicle-associated protein, Fenestrated endothelial-linked structure protein, Plasmalemma vesicle protein 1, PV-1

2 Images

Flow Cytometry - Anti-PLVAP/PV-1 antibody [174/2] (AB81719)

Flow Cyt

Unknown

Flow Cytometry - Anti-PLVAP/PV-1 antibody [174/2] (AB81719)

Overlay histogram showing HL60 cells stained with ab81719 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab81719, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HL60 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Immunocytochemistry/ Immunofluorescence - Anti-PLVAP/PV-1 antibody [174/2] (AB81719)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PLVAP/PV-1 antibody [174/2] (AB81719)

ab81719 at 1/50 dilution staining PLVAP/PV-1 in human tonsil by Immunofluorescence.

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

174/2

亚型

IgG1

不含载体蛋白

反应种属

Human

应用

Flow Cyt, ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/50", "FlowCyt-species-notes": "<p><a href='/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein G

纯化说明

ab81719 is 0.2µM filtered.

存储溶液

Preservative: 0.02% Sodium azide Constituents: PBS, 0.1% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

PLVAP also known as PV-1 is a transmembrane protein that plays a significant role in organizing the stomatal and fenestral diaphragms of endothelial cells. It weighs approximately 60 kDa. This protein is mainly expressed in fenestrated capillaries and sinusoidal endothelium where it forms diaphragms and contributes to the permeability and ultrastructure of capillary beds. PLVAP's role in endothelial cells highlights its importance in maintaining vascular permeability and cellular structures.

Biological function summary

The functions of PLVAP are significant in maintaining an organized structure within endothelial cells. It forms part of the complex structure that comprises the diaphragms of endothelial fenestrae transendothelial channels and caveolae. These structures are necessary for regulating fluid exchange and molecular transport between blood and tissue. The presence of PLVAP at these sites signals its role in modulating vascular barrier functions essential for efficient blood-tissue communication.

Pathways

Researchers have identified PLVAP's involvement in important vascular and endothelial pathways. It plays a critical part in the VEGF signaling pathway which regulates vascular permeability and endothelial cell behavior. PLVAP is closely associated with other proteins like VE-cadherin and claudin-5 that ensure cohesion and permeability in endothelial cells. In pathways regulating vascular permeability PLVAP's presence helps mediate fluid exchange and maintain tissue homeostasis.

PLVAP associates with conditions involving vascular dysregulation. Hypo- or hyper-expression of PLVAP can relate to the pathogenesis of cancer and inflammatory diseases. In cancer changes in PLVAP expression might contribute to altered tumor vasculature and metastasis often in association with proteins like VEGF and VEGFR-2. Furthermore inflammatory diseases such as atherosclerosis see changes in the protein expression levels that might disrupt vascular integrity linking them with proteins involved in inflammatory responses like ICAM-1 and VCAM-1.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Endothelial cell-specific membrane protein involved in the formation of the diaphragms that bridge endothelial fenestrae. It is also required for the formation of stomata of caveolae and transendothelial channels. Functions in microvascular permeability, endothelial fenestrae contributing to the passage of water and solutes and regulating transcellular versus paracellular flow in different organs. Plays a specific role in embryonic development.

See full target information PLVAP

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文献 (14)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 26: PubMed40429837

2025

Biosynthesized Calcium Peroxide Nanoparticles as a Multifunctional Platform for Liver Cancer Therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Sen Wu,Siqi Li,Xin Xia,Gen Zhang,Ting Wang

Small methods 9:e2401588 PubMed39871784

2025

Human Dermal Microvascular Arterial and Venous Blood Endothelial Cells and Their Use in Bioengineered Dermo-Epidermal Skin Substitutes.

Applications

Unspecified application

Species

Unspecified reactive species

Dominic Rütsche,Monica Nanni,Phil Cheng,Nicolà Caflisch,Aizhan Tastanova,Céline Jenni,Mitchell P Levesque,Ueli Moehrlen,Agnes S Klar,Thomas Biedermann

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2302940 PubMed38115754

2023

Human Pluripotent Stem Cells Derived Endothelial Cells Repair Choroidal Ischemia.

Applications

Unspecified application

Species

Unspecified reactive species

Mengda Li,Peiliang Wang,Si Tong Huo,Hui Qiu,Chendi Li,Siyong Lin,Libin Guo,Yicong Ji,Yonglin Zhu,Jinyang Liu,Jianying Guo,Jie Na,Yuntao Hu

Cell 186:5910-5924.e17 PubMed38070509

2023

A tridimensional atlas of the developing human head.

Applications

IHC - Wmt

Species

Human

Raphael Blain,Gérard Couly,Eimad Shotar,Joséphine Blévinal,Maryne Toupin,Anais Favre,Ali Abjaghou,Megumi Inoue,Edwin Hernández-Garzón,Frédéric Clarençon,Frédéric Chalmel,Séverine Mazaud-Guittot,Paolo Giacobini,Yorick Gitton,Alain Chédotal

iScience 26:107966 PubMed37810232

2023

The senescent secretome drives PLVAP expression in cultured human hepatic endothelial cells to promote monocyte transmigration.

Applications

Unspecified application

Species

Unspecified reactive species

Alex L Wilkinson,Samuel Hulme,James I Kennedy,Emily R Mann,Paul Horn,Emma L Shepherd,Kelvin Yin,Marco Y W Zaki,Gareth Hardisty,Wei-Yu Lu,Pia Rantakari,David H Adams,Marko Salmi,Matthew Hoare,Daniel A Patten,Shishir Shetty

Experimental & molecular medicine 55:628-642 PubMed36914857

2023

Single-cell sequencing reveals that endothelial cells, EndMT cells and mural cells contribute to the pathogenesis of cavernous malformations.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Ren,Xiao Xiao,Ruofei Li,Cheng Lv,Yu Zhang,Leiming Wang,Tao Hong,Hongqi Zhang,Yibo Wang

Nature methods 20:149-161 PubMed36550275

2022

Bioprinted 3D outer retina barrier uncovers RPE-dependent choroidal phenotype in advanced macular degeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Min Jae Song,Russ Quinn,Eric Nguyen,Christopher Hampton,Ruchi Sharma,Tea Soon Park,Céline Koster,Ty Voss,Carlos Tristan,Claire Weber,Anju Singh,Roba Dejene,Devika Bose,Yu-Chi Chen,Paige Derr,Kristy Derr,Sam Michael,Francesca Barone,Guibin Chen,Manfred Boehm,Arvydas Maminishkis,Ilyas Singec,Marc Ferrer,Kapil Bharti

iScience 25:104383 PubMed35633941

2022

Immortalized human choroid plexus endothelial cells enable an advanced endothelial-epithelial two-cell type model of the choroid plexus.

Applications

Unspecified application

Species

Unspecified reactive species

Walter Muranyi,Christian Schwerk,Rosanna Herold,Carolin Stump-Guthier,Marko Lampe,Petra Fallier-Becker,Christel Weiß,Carsten Sticht,Hiroshi Ishikawa,Horst Schroten

Progress in retinal and eye research 87:100994 PubMed34280556

2021

Exploring the choroidal vascular labyrinth and its molecular and structural roles in health and disease.

Applications

Unspecified application

Species

Unspecified reactive species

J Brinks,E H C van Dijk,I Klaassen,R O Schlingemann,S M Kielbasa,E Emri,P H A Quax,A A Bergen,O C Meijer,C J F Boon

European journal of pharmacology 854:240-246 PubMed31026444

2019

An anti-PLVAP antibody suppresses laser-induced choroidal neovascularization in monkeys.

Applications

Unspecified application

Species

Unspecified reactive species

Yasuhiro Nakagami,Emiko Hatano,Yuichi Chayama,Tatsuya Inoue

View all publications

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Anti-PLVAP/PV-1 抗体 [174/2]

Anti-PLVAP/PV-1 antibody [174/2]

Flow Cytometry - Anti-PLVAP/PV-1 antibody [174/2] (AB81719)

Immunocytochemistry/ Immunofluorescence - Anti-PLVAP/PV-1 antibody [174/2] (AB81719)

关键信息

宿主种属

克隆

克隆号

亚型

不含载体蛋白

反应种属

应用

免疫原