AB248837

重组Anti-PLSCR3抗体[EPR9242] - BSA and Azide free

Anti-PLSCR3 antibody [EPR9242] - BSA and Azide free

RabMAb
Recombinant
了解详情

Be the first to review this product! Submit a review

(0 Publication)

Rabbit Recombinant Monoclonal PLSCR3 antibody. Carrier free. Suitable for WB and reacts with Human samples.

查看别名

Phospholipid scramblase 3, PL scramblase 3, Ca(2+)-dependent phospholipid scramblase 3, PLSCR3

1 Images

Unknown

Western blot - Anti-PLSCR3 antibody [EPR9242] - BSA and Azide free (AB248837)

This data was developed using ab137128, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-PLSCR3 antibody [EPR9242] (<a href='/products/primary-antibodies/plscr3-antibody-epr9242-ab137128'>ab137128</a>) at 1/1000 dilution

Lane 1:

Human fetal lung lysate at 10 µg

Lane 2:

BxPC 3 lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 32 kDa

false

不同偶联物与剂型 (1)

Unconjugated

Anti-PLSCR3 antibody [EPR9242]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR9242

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

产品详情

ab248837 is the carrier-free version of ab137128.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

style

left-column

性能和储存信息

形式

Liquid

纯化工艺

Affinity purification

存储溶液

pH: 7.2 - 7.4 Constituents: PBS

运输条件

Blue Ice

储存信息

Do Not Freeze

style

left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

PLSCR3 also known as phospholipid scramblase 3 plays a role in the translocation of phospholipids across cell membranes. It weighs approximately 37 kDa and is closely associated with the mitoplast. This protein is found mainly in the mitochondria heart liver and skeletal muscle tissues. PLSCR3 is vital for maintaining the asymmetry of phospholipid distribution in cellular membranes important for cell viability.

Biological function summary

The activity of PLSCR3 in the mitochondria is significant in apoptosis and lipid metabolism processes. As a part of the scramblase family it collaborates with proteins in the Bcl-2 family to modulate apoptosis. This interaction is important for regulating the release of cytochrome c an important step in the intrinsic pathway of apoptosis. The phospholipid redistribution function of PLSCR3 supports the remodeling of mitochondrial membranes during metabolic processes.

Pathways

PLSCR3 influences the apoptosis and phospholipid signaling pathways. It interacts with proteins such as Bax and Bak within the apoptosis pathway facilitating the proper execution of cell death during stress responses. In the phospholipid signaling pathway PLSCR3 coordinates with phosphatidylserine and other lipids to regulate cellular signaling processes and membrane dynamics affecting cellular responses to environmental changes.

Mutations or dysregulation of PLSCR3 can contribute to metabolic disorders and certain types of cancer. The protein's role in apoptosis links it to disorders like cardiomyopathy where regulated cell death is important for heart muscle maintenance. Additionally in cancer abnormal PLSCR3 activity impacts cell growth and survival connecting it to oncogenes like MYC which can drive uncontrolled cell proliferation. Understanding PLSCR3's role helps develop potential therapeutic strategies for these conditions.

style

left-column

style

left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

靶点信息

Catalyzes calcium-induced ATP-independent rapid bidirectional and non-specific movement of the phospholipids (lipid scrambling or lipid flip-flop) between the inner and outer membrane of the mitochondria (PubMed : 14573790, PubMed : 17226776, PubMed : 18358005, PubMed : 29337693, PubMed : 31769662). Plays an important role in mitochondrial respiratory function, morphology, and apoptotic response (PubMed : 12649167, PubMed : 14573790, PubMed : 17226776, PubMed : 18358005). Mediates the translocation of cardiolipin from the mitochondrial inner membrane to outer membrane enhancing t-Bid induced cytochrome c release and apoptosis (PubMed : 14573790, PubMed : 17226776, PubMed : 18358005). Enhances TNFSF10-induced apoptosis by regulating the distribution of cardiolipin in the mitochondrial membrane resulting in increased release of apoptogenic factors and consequent amplification of the activity of caspases (PubMed : 18491232). Regulates cardiolipin de novo biosynthesis and its resynthesis (PubMed : 16939411).

See full target information PLSCR3

style

left-column

style

left-column

style

right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组Anti-PLSCR3抗体[EPR9242] - BSA and Azide free