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重组RabMAb

重组Anti-PLK1抗体[EPR19534] - BSA and Azide free (ab223142)

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Immunocytochemistry/ Immunofluorescence - Anti-PLK1 antibody [EPR19534] - BSA and Azide free (ab223142)
  • Flow Cytometry (Intracellular) - Anti-PLK1 antibody [EPR19534] - BSA and Azide free (ab223142)
  • Flow Cytometry (Intracellular) - Anti-PLK1 antibody [EPR19534] - BSA and Azide free (ab223142)
  • Immunoprecipitation - Anti-PLK1 antibody [EPR19534] - BSA and Azide free (ab223142)
  • Anti-PLK1 antibody [EPR19534] - BSA and Azide free (ab223142)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19534] to PLK1 - BSA and Azide free
  • Suitable for: WB, IP, ICC/IF, Flow Cyt (Intra)
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 594 Alexa Fluor® 647 APC PE Unconjugated

概述

  • 产品名称

    Anti-PLK1抗体[EPR19534] - BSA and Azide free
    参阅全部 PLK1 一抗

  • 描述

    兔单克隆抗体[EPR19534] to PLK1 - BSA and Azide free

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, IP, ICC/IF, Flow Cyt (Intra)more details

  • 种属反应性

    与反应: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: HeLa, K562, PC-3 and HepG2 whole cell lysates. ICC/IF: HeLa cells. Flow Cyt (intra): HeLa and K562 cells. IP: K562 whole cell lysate.

  • 常规说明

    ab223142 is the carrier-free version of ab189139.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab223142于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
Use at an assay dependent concentration. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).
IP
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
Flow Cyt (Intra)
Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
说明
WB
Use at an assay dependent concentration. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).
IP
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
Flow Cyt (Intra)
Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

靶标

  • 功能

    Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC/C inhibitors, and the regulation of mitotic exit and cytokinesis. Required for recovery after DNA damage checkpoint and entry into mitosis. Required for kinetochore localization of BUB1B. Phosphorylates SGOL1. Required for spindle pole localization of isoform 3 of SGOL1 and plays a role in regulating its centriole cohesion function. Phosphorylates BORA, and thereby promotes the degradation of BORA. Contributes to the regulation of AURKA function. Regulates TP53 stability through phosphorylation of TOPORS.

  • 组织特异性

    Placenta and colon.

  • 序列相似性

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.
    Contains 2 POLO box domains.
    Contains 1 protein kinase domain.

  • 发展阶段

    Accumulates to a maximum during the G2 and M phases, declines to a nearly undetectable level following mitosis and throughout G1 phase, and then begins to accumulate again during S phase.

  • 翻译后修饰

    Catalytic activity is enhanced by phosphorylation of Thr-210. Phosphorylation at Thr-210 is first detected on centrosomes in the G2 phase of the cell cycle, peaks in prometaphase and gradually disappears from centrosomes during anaphase.
    Autophosphorylation and phosphorylation of Ser-137 may not be significant for the activation of PLK1 during mitosis, but may enhance catalytic activity during recovery after DNA damage checkpoint.
    Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) in anaphase and following DNA damage, leading to its degradation by the proteasome. Ubiquitination is mediated via its interaction with FZR1/CDH1. Ubiquitination and subsequent degradation prevents entry into mitosis and is essential to maintain an efficient G2 DNA damage checkpoint.

  • 细胞定位

    Nucleus. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > centrosome. During early stages of mitosis, the phosphorylated form is detected on centrosomes and kinetochores. Localizes to the outer kinetochore. Presence of SGOL1 and interaction with the phosphorylated form of BUB1 is required for the kinetochore localization.
  • Information by UniProt

  • 数据库链接

    • 别名

      • Cell cycle regulated protein kinase antibody
      • PLK 1 antibody
      • PLK antibody
      • PLK-1 antibody
      • plk1 antibody
      • PLK1_HUMAN antibody
      • Polo like kinase 1 antibody
      • Polo-like kinase 1 antibody
      • Serine/threonine protein kinase 13 antibody
      • Serine/threonine protein kinase PLK1 antibody
      • Serine/threonine-protein kinase 13 antibody
      • Serine/threonine-protein kinase PLK1 antibody
      • STPK 13 antibody
      • STPK13 antibody
      see all

    图片

    • Immunocytochemistry/ Immunofluorescence - Anti-PLK1 antibody [EPR19534] - BSA and Azide free (ab223142)
      Immunocytochemistry/ Immunofluorescence - Anti-PLK1 antibody [EPR19534] - BSA and Azide free (ab223142)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling PLK1 with ab189139 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

      Confocal image showing strong signal staining on midbody and kinetochore of HeLa cell line.

      The nuclear counterstain is DAPI (blue).

      Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

       

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab189139).

    • Flow Cytometry (Intracellular) - Anti-PLK1 antibody [EPR19534] - BSA and Azide free (ab223142)
      Flow Cytometry (Intracellular) - Anti-PLK1 antibody [EPR19534] - BSA and Azide free (ab223142)

      Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed  HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling  PLK1 with ab189139 at 1/60 dilution (red) compared with a Rabbit IgG,monoclonal [EPR25A]-Isotype control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti Rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody. 

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab189139). 

       

       

    • Flow Cytometry (Intracellular) - Anti-PLK1 antibody [EPR19534] - BSA and Azide free (ab223142)
      Flow Cytometry (Intracellular) - Anti-PLK1 antibody [EPR19534] - BSA and Azide free (ab223142)

      Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed  K562 (Human chronic myelogenous leukemia cell line from bone marrow) cells labeling  PLK1 with ab189139 at 1/60 dilution (red) compared with a Rabbit IgG,monoclonal [EPR25A]-Isotype control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti Rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody. 

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab189139). 

       

       

    • Immunoprecipitation - Anti-PLK1 antibody [EPR19534] - BSA and Azide free (ab223142)
      Immunoprecipitation - Anti-PLK1 antibody [EPR19534] - BSA and Azide free (ab223142)

      PLK1 was immunoprecipitated from 0.35 mg of K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate with ab189139 at 1/30 dilution.

      Western blot was performed from the immunoprecipitate using ab189139 at 1/1000 dilution.

      VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

      Lane 1: K562 whole cell lysate, 10µg (Input).

      Lane 2: ab189139 IP in K562 whole cell lysate.

      Lane 3: Rabbit IgG,monoclonal [EPR25A]- Isotype Control (ab172730) instead of ab189139 in K562 whole cell lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 30 seconds.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab189139).

    • Anti-PLK1 antibody [EPR19534] - BSA and Azide free (ab223142)
      Anti-PLK1 antibody [EPR19534] - BSA and Azide free (ab223142)

    文献 (0)

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