Anti-PLK1抗体(ab109777)
Key features and details
- Rabbit polyclonal to PLK1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
-
产品名称
Anti-PLK1抗体
参阅全部 PLK1 一抗 -
描述
兔多克隆抗体to PLK1 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Cow
-
免疫原
Recombinant Human PLK1.
-
阳性对照
- Raji whole cell lysate; Human Brain (cortex), Human Placenta and Human Skin tissues
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 0.184% Tris glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab109777于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 68 kDa.
|
|
| IHC-P |
Use a concentration of 10 µg/ml.
|
| 说明 |
|---|
|
WB
1/500 - 1/3000. Predicted molecular weight: 68 kDa. |
|
IHC-P
Use a concentration of 10 µg/ml. |
靶标
-
功能
Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC/C inhibitors, and the regulation of mitotic exit and cytokinesis. Required for recovery after DNA damage checkpoint and entry into mitosis. Required for kinetochore localization of BUB1B. Phosphorylates SGOL1. Required for spindle pole localization of isoform 3 of SGOL1 and plays a role in regulating its centriole cohesion function. Phosphorylates BORA, and thereby promotes the degradation of BORA. Contributes to the regulation of AURKA function. Regulates TP53 stability through phosphorylation of TOPORS. -
组织特异性
Placenta and colon. -
序列相似性
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.
Contains 2 POLO box domains.
Contains 1 protein kinase domain. -
发展阶段
Accumulates to a maximum during the G2 and M phases, declines to a nearly undetectable level following mitosis and throughout G1 phase, and then begins to accumulate again during S phase. -
翻译后修饰
Catalytic activity is enhanced by phosphorylation of Thr-210. Phosphorylation at Thr-210 is first detected on centrosomes in the G2 phase of the cell cycle, peaks in prometaphase and gradually disappears from centrosomes during anaphase.
Autophosphorylation and phosphorylation of Ser-137 may not be significant for the activation of PLK1 during mitosis, but may enhance catalytic activity during recovery after DNA damage checkpoint.
Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) in anaphase and following DNA damage, leading to its degradation by the proteasome. Ubiquitination is mediated via its interaction with FZR1/CDH1. Ubiquitination and subsequent degradation prevents entry into mitosis and is essential to maintain an efficient G2 DNA damage checkpoint. -
细胞定位
Nucleus. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > centrosome. During early stages of mitosis, the phosphorylated form is detected on centrosomes and kinetochores. Localizes to the outer kinetochore. Presence of SGOL1 and interaction with the phosphorylated form of BUB1 is required for the kinetochore localization. - Information by UniProt
-
数据库链接
- Entrez Gene: 538238 Cow
- Entrez Gene: 5347 Human
- Entrez Gene: 18817 Mouse
- Entrez Gene: 25515 Rat
- Omim: 602098 Human
- SwissProt: Q2TA25 Cow
- SwissProt: P53350 Human
- SwissProt: Q07832 Mouse
see all -
别名
- Cell cycle regulated protein kinase antibody
- PLK 1 antibody
- PLK antibody
see all
图片
-
Western blot - Anti-PLK1 antibody (ab109777)Anti-PLK1 antibody (ab109777) at 1/1000 dilution + Raji whole cell lysate at 30 µg
Predicted band size: 68 kDa
7.5% SDS-PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLK1 antibody (ab109777)ab109777, at 10 µg/ml, staining PLK1 in formalin-fixed, paraffin-embedded Human Brain cortex by Immunohistochemistry. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLK1 antibody (ab109777)ab109777, at 10 µg/ml, staining PLK1 in formalin-fixed, paraffin-embedded Human Placenta tissue by Immunohistochemistry. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLK1 antibody (ab109777)ab109777, at 10 µg/ml, staining PLK1 in formalin-fixed, paraffin-embedded Human Skin by Immunohistochemistry.
数据表及文件
-
SDS download
-
Datasheet download
文献 (4)
ab109777 被引用在 4 文献中.
- Peng W et al. The intercorrelation among CCT6A, CDC20, CCNB1, and PLK1 expressions and their clinical value in papillary thyroid carcinoma prognostication. J Clin Lab Anal 36:e24609 (2022). IHC ; Human . PubMed: 35838025
- Zhang Z et al. Comprehensive analysis of differentially expressed genes associated with PLK1 in bladder cancer. BMC Cancer 17:861 (2017). PubMed: 29246203
- Diab A et al. Polo-like-kinase 1 is a proviral host factor for hepatitis B virus replication. Hepatology 66:1750-1765 (2017). PubMed: 28445592
- Telkoparan P et al. Coiled-coil domain containing protein 124 is a novel centrosome and midbody protein that interacts with the Ras-guanine nucleotide exchange factor 1B and is involved in cytokinesis. PLoS One 8:e69289 (2013). Human . PubMed: 23894443