Anti-PLK1 抗体 [36-298]
Anti-PLK1 antibody [36-298]
4
(3 Reviews)
|
(44 Publications)
Mouse Monoclonal PLK1 antibody. Suitable for ICC, Flow Cyt (Intra), WB and reacts with Mouse, Human, Recombinant full length protein samples. Cited in 44 publications.
查看别名
PLK, PLK1, Serine/threonine-protein kinase PLK1, Polo-like kinase 1, Serine/threonine-protein kinase 13, PLK-1, STPK13
- ICC
Unknown
Immunocytochemistry - Anti-PLK1 antibody [36-298] (AB17057)
Immunofluoresence using ab17057 and either HeLa S3, NIH/3T3 (Mouse embyro fibroblast cell line) or U-2 OS (Human bone osteosarcoma epithelial cell line) cells.
- Flow Cyt (Intra)
Supplier Data
Flow Cytometry (Intracellular) - Anti-PLK1 antibody [36-298] (AB17057)
Flow cytometry overlay histogram showing HeLa cells stained with ab17057 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab17057) (1x106 in 100 μl at 5 μg/ml) for 30 min at 22°C.
The secondary antibody Goat anti-mouse IgG H&L (Alexa Fluor® 488, pre-adsorbed) (ab150117) was used at 1/4000 dilution for 30 min at 22°C.
Isotype control antibody (black line) was mouse IgG1 kappa; (ab170190) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.
This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min) / permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.
- ICC
Lab
Immunocytochemistry - Anti-PLK1 antibody [36-298] (AB17057)
ab17057 staining PLK1 in HeLa cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab17057 at 1µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.
- Flow Cyt (Intra)
Supplier Data
Flow Cytometry (Intracellular) - Anti-PLK1 antibody [36-298] (AB17057)
Flow cytometry overlay histogram showing HeLa cells stained with ab17057 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab17057) (1x106 in 100 μl at 5 μg/ml) for 30 min at 22°C.
The secondary antibody Goat anti-mouse IgG H&L (Alexa Fluor® 488, pre-adsorbed) (ab150117) was used at 1/4000 dilution for 30 min at 22°C.
Isotype control antibody (black line) was mouse IgG1 kappa; (ab170190) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.
This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min) / permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.
- ICC
Collaborator
Immunocytochemistry - Anti-PLK1 antibody [36-298] (AB17057)
In panel one HeLa (Human epithelial cell line from cervix adenocarcinoma) cells were stained with ab17057 (green) and DAPI. In the second panel, cells were stained with ab17057 (green) and SH-CREST (red), which stains the centromeres. Fix 30 minutes on ice in 4% formaldehyde in PEM. Quench autofluorescence 2 x 5 minutes with 1 mg/ml Na borohydride or 100 mM ammonium chloride in PEM. Permeabilized for 30 minutes with 0.5% TX-100 in PEM. Block 30 minutes in 5% milk in TBST. Primary antibody incubated overnight at 4°C diluted 1/400 in 5% milk in TBST. Secondary antibody incubated 1 hour at RT diluted in 5% milk in TBST. Post-fix 20 minutes on ice in 4% formaldehyde in PEM. Quench autofluorescence 2 x 5 minutes with ammonium chloride in PEM. Counterstain with DAPI in TBST. Mount with ProLong Gold antifade reagent from Invitrogen.
Notes : Ample washing between each step.
TBST = Tris buffered saline + 0.1% Tween. PEM = 80 mM K-PIPES, pH 6.8, 5 mM EGTA, 2 mM MgCl2.
This image is courtesy of Scott Slattery and Mike Mancini
- WB
Project837****
Western blot - Anti-PLK1 antibody [36-298] (AB17057)
All lanes:
Western blot - Anti-PLK1 antibody [36-298] (ab17057) at 1 µg/mL
All lanes:
HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate at 20 µg
Secondary
All lanes:
Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (<a href='/products/secondary-antibodies/rabbit-mouse-igg-h-l-hrp-ab6728'>ab6728</a>) at 1/5000 dilution
Predicted band size: 68 kDa
Observed band size: 66 kDa
true
Exposure time: 2min
- WB
Unknown
Western blot - Anti-PLK1 antibody [36-298] (AB17057)
10% SDS-PAGE gel.
All lanes:
Western blot - Anti-PLK1 antibody [36-298] (ab17057)
Lane 1:
Recombinant PLK1
Lane 2:
U-2 OS (Human bone osteosarcoma epithelial cell line) cell extract
Lane 3:
HeLa S3 cell extract
Predicted band size: 68 kDa
Observed band size: 66 kDa
false
- WB
CiteAb
Western blot - Anti-PLK1 antibody [36-298] (AB17057)
PLK1 western blot using anti-PLK1 antibody [36-298] ab17057. Publication image and figure legend from Tan, E. P., Caro, S., et al., 2013, J Biol Chem, PubMed 23946484.
ab17057 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab17057 please see the product overview.
Disrupted O-GlcNAc cycling alters spindle protein expression. a spindle schematic of the locations and area of phosphorylation of mitotic kinases AurA, AurB, and PLK1. b, M phase synchronized GFP/OGT/OGA gain of function or TMG-treated cells were Western blotted for PLK1, AurA, and actin. c, DNA (blue), α-tubulin (green), and AurA (red) were confocal imaged at M phase in OGT/OGA gain of function cells. d, DNA (blue), α-tubulin (green), and PLK1 (red) were confocal imaged at M phase in OGT/OGA gain of function cells. e, Western blot of CPC proteins AurB, INCENP, borealin, surviving, and actin as a load control (f) DNA (blue), INCENP (green), and AurB (red) were confocal imaged at M phase in OGT/OGA gain of function cells. g, AurB width was quantified using ImageJ (mean ± S.E., replicate number (n) : nControl = 45, nOGT = 35, nOGA = 43, *, p < 0.005 between Control versus OGA). h, DNA (blue), INCENP (green), and survivin (red) were confocal imaged at M phase in OGT/OGA gain of function cells.
false
反应性数据
产品详情
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
性能和储存信息
形式
纯化工艺
存储溶液
运输条件
推荐的短期储存时间
推荐的短期储存条件
推荐的长期储存条件
分装信息
储存信息
产品实验方案
- Visit the General protocols
- Visit the Troubleshooting
靶点信息
文献 (44)
Recent publications for all applications. Explore the full list and refine your search
Nature communications 16:7898 PubMed40849432
2025
Applications
Unspecified application
Species
Unspecified reactive species
General physiology and biophysics 44:259-274 PubMed40567070
2025
Applications
Unspecified application
Species
Unspecified reactive species
Journal of inflammation research 18:4381-4394 PubMed40162075
2025
Applications
Unspecified application
Species
Unspecified reactive species
Scientific reports 15:9280 PubMed40102598
2025
Applications
Unspecified application
Species
Unspecified reactive species
Life science alliance 8: PubMed39433344
2024
Applications
Unspecified application
Species
Unspecified reactive species
The EMBO journal 43:2424-2452 PubMed38714893
2024
Applications
Unspecified application
Species
Unspecified reactive species
Cell proliferation 57:e13590 PubMed38084791
2023
Applications
Unspecified application
Species
Unspecified reactive species
The EMBO journal 42:e112630 PubMed37712330
2023
Applications
Unspecified application
Species
Unspecified reactive species
Cell death & disease 14:571 PubMed37640723
2023
Applications
Unspecified application
Species
Unspecified reactive species
Frontiers in oncology 12:985827 PubMed36818671
2023
Applications
Unspecified application
Species
Unspecified reactive species
Abcam Product Promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com