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Anti-Plexin B1抗体(ab39717)

Reviews (2)Q&A (1)References (3)
Western blot - Anti-Plexin B1 antibody (ab39717)
  • Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Plexin B1 antibody (ab39717)

Key features and details

  • Rabbit polyclonal to Plexin B1
  • Suitable for: IHC-FoFr, WB
  • Reacts with: Mouse, Rat
  • Isotype: IgG

概述

  • 产品名称

    Anti-Plexin B1抗体
    参阅全部 Plexin B1 一抗

  • 描述

    兔多克隆抗体to Plexin B1

  • 宿主

    Rabbit

  • 特异性

    PLexin B1 is readily cleaved at the intra- extra- cellular boundary (circa aa.1305/1306) likely giving rise to the several different band sizes routinely observed in WB, depending on the isoform that has been cleaved.

  • 经测试应用

    适用于: IHC-FoFr, WBmore details

  • 种属反应性

    与反应: Mouse, Rat
    预测可用于: Human

  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 300 - 400 of Human Plexin B1.

    参阅Abcam的专有抗源政策 (Peptide available as ab41062.)

  • 阳性对照

    • WB: Mouse and rat liver tissue lysates.

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • 存储溶液

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...

  • 纯度

    Immunogen affinity purified

  • 克隆

    多克隆

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab39717于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-FoFr
1/1000.
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 171 kDa (predicted molecular weight: 233 kDa).
说明
IHC-FoFr
1/1000.
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 171 kDa (predicted molecular weight: 233 kDa).

靶标

  • 功能

    Receptor for SEMA4D. Plays a role in RHOA activation and subsequent changes of the actin cytoskeleton. Plays a role in axon guidance, invasive growth and cell migration.

  • 组织特异性

    Highly expressed in fetal kidney, and at slightly lower levels in fetal brain, lung and liver.

  • 序列相似性

    Belongs to the plexin family.
    Contains 3 IPT/TIG domains.
    Contains 1 Sema domain.

  • 翻译后修饰

    Phosphorylated on tyrosine residues by ERBB2 and MET upon SEMA4D binding.
    Proteolytic processing favors heterodimerization with PLXNB2 and SEMA4D binding.

  • 细胞定位

    Secreted and Cell membrane.
  • Information by UniProt

  • 数据库链接

    • 别名

      • KIAA0407 antibody
      • Plexin 5 antibody
      • Plexin B1 antibody
      • Plexin-B1 antibody
      • PLXB1_HUMAN antibody
      • PLXN5 antibody
      • Plxnb1 antibody
      • Semaphorin receptor antibody
      • Semaphorin receptor SEP antibody
      • SEP antibody
      see all

    图片

    • Western blot - Anti-Plexin B1 antibody (ab39717)
      Western blot - Anti-Plexin B1 antibody (ab39717)
      All lanes : Anti-Plexin B1 antibody (ab39717) at 1 µg/ml

      Lane 1 : Mouse liver tissue lysate
      Lane 2 : Rat liver tissue lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution

      Performed under reducing conditions.

      Predicted band size: 233 kDa
      Observed band size: 171 kDa why is the actual band size different from the predicted?


      Exposure time: 1 minute
    • Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Plexin B1 antibody (ab39717)
      Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Plexin B1 antibody (ab39717)This image is courtesy of Sophie Pezet, CNRS, Paris, France

      Immunohistochemistical detection of Plexin B1 with antibody ab39717 on PFA perfusion-fixed rat brain sections. Primary antibody ab39717 was diluted 1/1000 and incubated for 18 hours @ 20°C in PBS + 0.3 % Triton X100.  Secondary antibody: goat anti-rabbit Alexa Fluor 488 conjugated (1/1000). Immunostaining obtained in the hypothalamus, next to the rat brain ventricle. Immunostaining was performed using the `free floating` technique, using direct fluorescence. The image shows staining observed in glial cells. Some staining was observed on cells bordering the ventricle, but no other type of cells such as neurons. This is consistent with the literature where immunoreactivity is published in glial cells and neurons & as suggestted by several studies, as by Ito Y (EMBO Rep. 2006 Jul7(7):704-9).

    文献 (3)

    发表研究结果有使用 ab39717？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab39717 被引用在 3 文献中.

    • Gerstmann K  et al. A balance of noncanonical Semaphorin signaling from the cerebrospinal fluid regulates apical cell dynamics during corticogenesis. Sci Adv 8:eabo4552 (2022). PubMed: 36399562
    • Wu JH  et al. Inhibition of Sema4D/PlexinB1 signaling alleviates vascular dysfunction in diabetic retinopathy. EMBO Mol Med 12:e10154 (2020). PubMed: 31943789
    • Daviaud N  et al. Impaired cortical neurogenesis in plexin-B1 and -B2 double deletion mutant. Dev Neurobiol 76:882-99 (2016). PubMed: 26579598

    客户评价及客户问答

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    1-3 of 3 Abreviews or Q&A

    Immunocytochemistry abreview for Anti-Plexin B1 antibody

     Good
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Sample
    Mouse Cultured Cells (Primary dissection of hippocampal neurons.)
    Specification
    Primary dissection of hippocampal neurons.
    Fixative
    Paraformaldehyde
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Michael Weible

    Verified customer

    提交于 Nov 08 2012

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Plexin B1 antibody

     Average
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Brain sections)
    Specification
    Brain sections
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    None
    Permeabilization
    No
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Sophie Pezet

    Verified customer

    提交于 Feb 24 2010

    Question

    Please see a request we have received from a customer


    Thank you for your email. Yes, I would be happy to test the antibodies and share the data with Abcam.


    If you could request a code for the following antibodies that would be great:



    1. Products catalogue number: Anti-Plexin B1 antibody (ab39717)
    2. Which application the customer would like to test: Immunocytochemistry (ICC)
    3. Which species: Mouse C57
    4. Their sample type: neurons cultured from dorsal root ganglia, trigeminal ganglia and hippocampus.


    1. Products catalogue number: Anti-Dlx2 antibody [4B9] (ab117546)
    2. Which application the customer would like to test: Immunocytochemistry (ICC)
    3. Which species: Mouse C57
    4. Their sample type: Adult neural stem/progenitor cells derived from the SVZ and cultured as neurospheres.

    1. Products catalogue number: Anti-MASH1/Achaete-scute homolog 1 antibody (ab38556)
    2. Which application the customer would like to test: Immunocytochemistry (ICC)
    3. Which species: Mouse C57
    4. Their sample type: Adult neural stem/progenitor cells derived from the SVZ and cultured as neurospheres.

    1. Products catalogue number: Anti-SOX2 antibody (ab97959)
    2. Which application the customer would like to test: Immunocytochemistry (ICC)
    3. Which species: Mouse C57
    4. Their sample type: Adult neural stem/progenitor cells derived from the SVZ and cultured as neurospheres.

    Read More
    Answer



    Expiration date: 2012-11-09


    I am very pleased to hear you would like to accept our offer and test

    ab38556 Anti-MASH1/Achaete-scute homolog 1 antibody
    ab39717 Anti-Plexin B1 antibody
    ab117546 Anti-Dlx2 antibody [4B9]



    in ICC. One code will give you: 1 free primary before the expiration date. To redeem this offer, please submit an Abreview for ICCand include this code in the "Additional Comments" section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
    For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.


    In addition, ab97959 Anti-SOX2 antibodyhas already been tested for mouse as well as for ICC. Therefore, itis not a testing-discount candidate.

    The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com