Anti-PLEKHA7抗体(ab224548)
Key features and details
- Rabbit polyclonal to PLEKHA7
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-PLEKHA7抗体 -
描述
兔多克隆抗体to PLEKHA7 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment corresponding to Human PLEKHA7 aa 250-400.
Database link: Q6IQ23 -
阳性对照
- WB: PLEKHA7 overexpression HEK-293T cell lysate. IHC-P: Human duodenum, kidney, prostate and pancreas tissue ICC/IF: A431 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab224548于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
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| WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 127 kDa.
|
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| IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 说明 |
|---|
|
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
|
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 127 kDa. |
|
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Required for zonula adherens biogenesis and maintenance. Acts via its interaction with KIAA1543/Nezha, which anchors microtubules at their minus-ends to zonula adherens, leading to recruit KIFC3 kinesin to junctional site. -
序列相似性
Contains 1 PH domain.
Contains 2 WW domains. -
细胞定位
Cell junction > adherens junction. Cytoplasm. Cytoplasm > cytoskeleton > centrosome. Localizes to zonula adherens, recruited via its interaction with CTNND1. - Information by UniProt
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数据库链接
- Entrez Gene: 144100 Human
- Omim: 612686 Human
- SwissProt: Q6IQ23 Human
- Unigene: 12332 Human
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别名
- DKFZp686M22243 antibody
- PH domain-containing family A member 7 antibody
- PKHA7_HUMAN antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-PLEKHA7 antibody (ab224548)
PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for PLEKHA7 (green) using ab224548 at 4 µg/ml in ICC/IF.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLEKHA7 antibody (ab224548)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pancreas tissue labelling PLEKHA7 with ab224548 at 1/200 dilution.
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Western blot - Anti-PLEKHA7 antibody (ab224548)All lanes : Anti-PLEKHA7 antibody (ab224548) at 1/100 dilution
Lane 1 : Vector only transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : PLEKHA7 overexpression HEK-293T cell lysate (Co-expressed with a C-terminal myc-DDK tag, ~3.1 kDa)
Predicted band size: 127 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLEKHA7 antibody (ab224548)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate tissue labelling PLEKHA7 with ab224548 at 1/200 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLEKHA7 antibody (ab224548) -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLEKHA7 antibody (ab224548)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human duodenum tissue labelling PLEKHA7 with ab224548 at 1/200 dilution.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab224548 尚未被引用在任何文献中。