Anti-PLCG 2 (phospho Y753)抗体(ab75659)
Key features and details
- Rabbit polyclonal to PLCG 2 (phospho Y753)
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-PLCG 2 (phospho Y753)抗体
参阅全部 PLCG 2 一抗 -
描述
兔多克隆抗体to PLCG 2 (phospho Y753) -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human PLCG 2 (phospho Y753).
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 50% Glycerol (glycerin, glycerine), PBS
Without Mg2+ and Ca2+ -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab75659 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab75659于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
1/100 - 1/200.
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| WB |
1/500 - 1/1000. Detects a band of approximately 148 kDa (predicted molecular weight: 148 kDa).
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| 说明 |
|---|
|
ICC/IF
1/100 - 1/200. |
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WB
1/500 - 1/1000. Detects a band of approximately 148 kDa (predicted molecular weight: 148 kDa). |
靶标
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功能
The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. It is a crucial enzyme in transmembrane signaling. -
序列相似性
Contains 1 C2 domain.
Contains 1 PH domain.
Contains 1 PI-PLC X-box domain.
Contains 1 PI-PLC Y-box domain.
Contains 2 SH2 domains.
Contains 1 SH3 domain. -
翻译后修饰
Phosphorylated on tyrosine residues; upon ligand-induced activation of a variety of growth factor receptors and immune system receptors. Increases phospholipase activity. - Information by UniProt
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数据库链接
- Entrez Gene: 5336 Human
- Omim: 600220 Human
- SwissProt: P16885 Human
- Unigene: 413111 Human
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别名
- 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 2 antibody
- 1-phosphatidylinositol-4 antibody
- 5-bisphosphate phosphodiesterase gamma-2 antibody
see all
图片
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Western blot - Anti-PLCG 2 (phospho Y753) antibody (ab75659)All lanes : Anti-PLCG 2 (phospho Y753) antibody (ab75659) at 1/500 dilution
Lane 1 : A431 cell extracts, untreated
Lane 2 : A431 cell extracts treated with EGF (200ng/ml, 5min)
Predicted band size: 148 kDa
Observed band size: 148 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-PLCG 2 (phospho Y753) antibody (ab75659)ab75659 at 1/100 dilution staining PLCG 2 (phospho Y753) in human Hela cells by ICC/IF.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab75659 被引用在 1 文献中.
- Sadeghi L et al. Differential B-Cell Receptor Signaling Requirement for Adhesion of Mantle Cell Lymphoma Cells to Stromal Cells. Cancers (Basel) 12:N/A (2020). PubMed: 32370190