重组Anti-PLCG 2抗体[EPR5914-34] (ab133522)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5914-34] to PLCG 2
- Suitable for: WB, IHC-P, Flow Cyt (Intra), IP
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-PLCG 2抗体[EPR5914-34]
参阅全部 PLCG 2 一抗 -
描述
兔单克隆抗体[EPR5914-34] to PLCG 2 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, Flow Cyt (Intra), IPmore details
不适用于: ICC/IF -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Raji and Ramos cell lysates; Human gastric adenocarcinoma tissue. Flow Cyt (intra): Human Peripheral Blood Mononuclear Cell (PBMC) cells
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数(KD)
KD = 3.00 x 10 -12 M Learn more about KD -
存储溶液
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR5914-34 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab133522于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/10000 - 1/50000. Predicted molecular weight: 148 kDa.
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Flow Cyt (Intra) |
1/1000.
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IP |
1/10000.
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说明 |
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WB
1/10000 - 1/50000. Predicted molecular weight: 148 kDa. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Flow Cyt (Intra)
1/1000. |
IP
1/10000. |
靶标
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功能
The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. It is a crucial enzyme in transmembrane signaling. -
序列相似性
Contains 1 C2 domain.
Contains 1 PH domain.
Contains 1 PI-PLC X-box domain.
Contains 1 PI-PLC Y-box domain.
Contains 2 SH2 domains.
Contains 1 SH3 domain. -
翻译后修饰
Phosphorylated on tyrosine residues; upon ligand-induced activation of a variety of growth factor receptors and immune system receptors. Increases phospholipase activity. - Information by UniProt
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数据库链接
- Entrez Gene: 5336 Human
- Omim: 600220 Human
- SwissProt: P16885 Human
- Unigene: 413111 Human
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别名
- 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 2 antibody
- 1-phosphatidylinositol-4 antibody
- 5-bisphosphate phosphodiesterase gamma-2 antibody
see all
图片
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All lanes : Anti-PLCG 2 antibody [EPR5914-34] (ab133522) at 1/10000 dilution
Lane 1 : Wild-type HAP1 cell lysate
Lane 2 : PLCG2 knockout HAP1 cell lysate
Lane 3 : Ramos cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 148 kDa
Observed band size: 148 kDaAnti-PLCG2 antibody [EPR5914-34] (ab133522) staining at 1/10000 dilution, shown in green; Mouse anti-CANX [CANX/1543] (ab238078) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab133522 was shown to bind specifically to PLCG2. A band was observed at 148 kDa in wild-type HAP1 cell lysates with no signal observed at this size in PLCG2 knockout cell line. To generate this image, wild-type and PLCG2 knockout HAP1 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLCG 2 antibody [EPR5914-34] (ab133522)
Immunohistochemistry analysis of Paraffin Embedded Human gastric adenocarcinoma tissue labelling PLCG2 with ab133522 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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This data was developed using ab248535, the same antibody clone in a different buffer formulation.
Western blot: antibody description (ab248535) staining at 10,000 dilution, shown in Black. In Western blot, ab248535 was shown to bind specifically to PLCG2. An enriched band was observed at 160 kDa in samples pulled down with ab248535 with no band observed in Isotype control sample. Following IP, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween$®$ 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times before development with a high-sensitivity ECL substrate kit and imaged. Secondary antibodies used was VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution.
All lanes : Anti-PLCG 2 antibody [EPR5914-34] - BSA and Azide free (ab248535) at 1/10000 dilution
Lane 1 : HAP1 Input cell lysate
Lane 2 : HAP1 pulldown with ab248535
Lane 3 : HAP1 pulldown with ab172730 Isotype control
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Observed band size: 160 kDa why is the actual band size different from the predicted?
Exposure time: 4 minutes -
Flow Cytometry analysis of Human Peripheral Blood Mononuclear Cell (PBMC) cells labeling PLCG 2 with purified ab133522 at 1/1000 dilution (1 µg/mL) (Red). Cells were fixed with 2% Paraformaldehyde and permeabilised with 0.1% Tween-20. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Left).
Cells were surface stained with anti-CD19 conjugated to PE/Cy7, then fixed with 2% PFA followed by intracellularly stained with rabbit IgG or ab133522. Gated on lymphocytes. -
All lanes : Anti-PLCG 2 antibody [EPR5914-34] (ab133522) at 1/10000 dilution
Lane 1 : Raji cell lysates
Lane 2 : Ramos cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-labelled Goat anti-Rabbit at 1/2000 dilution
Predicted band size: 148 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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