AB109129

重组Anti-Plastin L抗体[EPR4278]

Anti-Plastin L antibody [EPR4278]

RabMAb
Recombinant
了解详情

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(2 Publications)

Rabbit Recombinant Monoclonal Plastin L antibody. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications.

查看别名

PLS2, LCP1, Plastin-2, L-plastin, LC64P, Lymphocyte cytosolic protein 1, LCP-1

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plastin L antibody [EPR4278] (AB109129)

IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plastin L antibody [EPR4278] (AB109129)

Immunohistochemical staining of paraffin-embedded Human tonsil tissue using ab109129 at a dilution of 1/250.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-Plastin L antibody [EPR4278] (AB109129)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-Plastin L antibody [EPR4278] (AB109129)

Flow Cytometry analysis of MDA-MB-435S (Human mammary gland ductal carcinoma melanocyte) cells labeling Plastin L with purified ab109129 at 1/1000 dilution (1 μg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

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Western blot - Anti-Plastin L antibody [EPR4278] (AB109129)

All lanes:

Western blot - Anti-Plastin L antibody [EPR4278] (ab109129) at 1/10000 dilution

Lane 1:

THP-1 cell lysate at 10 µg

Lane 2:

Jurkat cell lysate at 10 µg

Lane 3:

K562 cell lysate at 10 µg

Predicted band size: 70 kDa

false

不同偶联物与剂型 (1)

Carrier free

Anti-Plastin L antibody [EPR4278] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR4278

亚型

IgG

不含载体蛋白

反应种属

Human

应用

IHC-P, WB, Flow Cyt (Intra)

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/1000", "FlowCytIntra-species-notes": "" } } }

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产品详情

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

储存信息

Stable for 12 months at -20°C

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Plastin L also known as L-Plastin or plastine is a type of actin-bundling protein involved in regulating cytoskeletal rearrangements. This protein has a mass of approximately 70 kDa and serves an essential role in maintaining cell structure and mobility by cross-linking actin filaments. It is predominantly found in hematopoietic cells such as leukocytes but also expressed in certain non-hematopoietic cells. In zebrafish studies plastin L has been important for understanding its conservation and function across species.

Biological function summary

Plastin L participates in cellular processes such as phagocytosis cell motility and immune responses by stabilizing actin filaments. It does not form part of a larger protein complex but interacts with actin to perform its functions. As an actin-binding protein it helps in the formation and maintenance of the cytoskeleton impacting the cell's mechanical properties and response to external stimuli.

Pathways

Plastin L plays significant roles in immune cell signaling and cytoskeletal rearrangements. It is involved in pathways such as the immune response and cell adhesion where it contributes to leukocyte emigration and inflammation. Plastin L is functionally related to proteins like talin and paxillin which are key in mediating signal transduction resulting in cytoskeletal remodeling.

Plastin L is associated with cancer and immune system disorders. Overexpression of plastin L correlates with various forms of cancer highlighting its role in tumor cell invasion and metastasis. Additionally it shows a connection with autoimmune conditions where deregulation of actin polymerization affects immune cell function. In these contexts proteins like cofilin interact with plastin L to modulate actin dynamics and cellular responses.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Actin-binding protein (PubMed : 16636079, PubMed : 17294403, PubMed : 28493397). Plays a role in the activation of T-cells in response to costimulation through TCR/CD3 and CD2 or CD28 (PubMed : 17294403). Modulates the cell surface expression of IL2RA/CD25 and CD69 (PubMed : 17294403).

See full target information LCP1

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

The British journal of dermatology 184:709-721 PubMed32794257

2020

Identification of proteins associated with development of metastasis from cutaneous squamous cell carcinomas (cSCCs) via proteomic analysis of primary cSCCs.

Applications

Unspecified application

Species

Unspecified reactive species

A Shapanis,C Lai,S Smith,G Coltart,M Sommerlad,J Schofield,E Parkinson,P Skipp,E Healy

Cell death & disease 8:e3060 PubMed28981098

2017

AP4 modulated by the PI3K/AKT pathway promotes prostate cancer proliferation and metastasis of prostate cancer via upregulating L-plastin.

Applications

Species

Human

Changhao Chen,Qingqing Cai,Wang He,Thomas B Lam,Jianxun Lin,Yue Zhao,Xu Chen,Peng Gu,Hao Huang,Miaoxin Xue,Hao Liu,Feng Su,Jian Huang,Jianping Zheng,Tianxin Lin

View all publications

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