重组Anti-Plastin L抗体[EPR4277] (ab109124)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plastin L antibody [EPR4277] (ab109124)](https://www.abcam.cn/ps/products/109/ab109124/Images/ab109124-541709-anti-plastin-l-antibody-epr4277-immunohistochemistry-rat-testis-muscle-low-expression-control.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plastin L antibody [EPR4277] (ab109124)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4277] to Plastin L
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-Plastin L抗体[EPR4277]
参阅全部 Plastin L 一抗 -
描述
兔单克隆抗体[EPR4277] to Plastin L -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt or IP -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- THP1, K562 and Jurkat whole cell lysate (ab7899); Human tonsil tissue WB: Rat spleen, Mouse spleen IHC-P: Mouse spleen, mouse colon, rat spleen
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR4277 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab109124于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 70 kDa.
|
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| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
(Heat to 98°C allow to cool for 10-20 min). |
| 说明 |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 70 kDa. |
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (Heat to 98°C allow to cool for 10-20 min). |
靶标
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功能
Actin-binding protein. Plays a role in the activation of T-cells in response to costimulation through TCR/CD3 and CD2 or CD28. Modulates the cell surface expression of IL2RA/CD25 and CD69. -
组织特异性
Detected in intestinal microvilli, hair cell stereocilia, and fibroblast filopodia, in spleen and other lymph node-containing organs. Expressed in peripheral blood T lymphocytes, neutrophils, monocytes, B lymphocytes, and myeloid cells. -
序列相似性
Contains 2 actin-binding domains.
Contains 4 CH (calponin-homology) domains.
Contains 2 EF-hand domains. -
翻译后修饰
Phosphorylated on a serine residue in response to costimulation through TCR/CD3 and CD2 or CD28. Serine phosphorylation promotes association with the actin cytoskeleton and targeting to peripheral cell projections.
The N-terminus is blocked. -
细胞定位
Cytoplasm > cytoskeleton. Cell junction. Cell projection. Cell projection > ruffle membrane. Relocalizes to the immunological synapse between peripheral blood T lymphocytes and antibody-presenting cells in response to costimulation through TCR/CD3 and CD2 or CD28. Associated with the actin cytoskeleton at membrane ruffles (By similarity). Relocalizes to actin-rich cell projections upon serine phosphorylation. - Information by UniProt
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数据库链接
- Entrez Gene: 3936 Human
- Entrez Gene: 18826 Mouse
- Entrez Gene: 306071 Rat
- Omim: 153430 Human
- SwissProt: P13796 Human
- SwissProt: Q61233 Mouse
- Unigene: 381099 Human
- Unigene: 710563 Human
see all -
别名
- CP64 antibody
- L plastin antibody
- L-plastin antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plastin L antibody [EPR4277] (ab109124)
Immunohistochemical analysis of paraffin-embedded Rat testis labelling Plastin L with ab109124 at 1/400 dilution (0.35 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Low expression control: Negligible staining on Rat testis.
The section was incubated with ab109124 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plastin L antibody [EPR4277] (ab109124)](https://www.abcam.cn/ps/products/109/ab109124/Images/ab109124-541707-anti-plastin-l-antibody-epr4277-immunohistochemistry-mouse-liver-low-expression-control.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plastin L antibody [EPR4277] (ab109124)Immunohistochemical analysis of paraffin-embedded Mouse liver labelling Plastin L with ab109124 at 1/400 dilution (0.35 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Low expression control: Negligible staining on mouse liver.
The section was incubated with ab109124 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plastin L antibody [EPR4277] (ab109124)](https://www.abcam.cn/ps/products/109/ab109124/Images/ab109124-541705-anti-plastin-l-antibody-epr4277-immunohistochemistry-mouse-testis-muscle-low-expression-control.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plastin L antibody [EPR4277] (ab109124)Immunohistochemical analysis of paraffin-embedded Mouse testis labelling Plastin L with ab109124 at 1/400 dilution (0.35 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Low expression control: Negligible staining on mouse testis.
The section was incubated with ab109124 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plastin L antibody [EPR4277] (ab109124)](https://www.abcam.cn/ps/products/109/ab109124/Images/ab109124-541703-anti-plastin-l-antibody-epr4277-immunohistochemistry-mouse-cardiac-muscle-low-expression-control.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plastin L antibody [EPR4277] (ab109124)Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle labelling Plastin L with ab109124 at 1/400 dilution (0.35 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Low expression control: no staining on the cardiac muscle in mouse heart, but staining the stroma immune cells.
The section was incubated with ab109124 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plastin L antibody [EPR4277] (ab109124)](https://www.abcam.cn/ps/products/109/ab109124/Images/ab109124-541701-anti-plastin-l-antibody-epr4277-immunohistochemistry-rat-spleen.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plastin L antibody [EPR4277] (ab109124)Immunohistochemical analysis of paraffin-embedded Rat spleen labelling Plastin L with ab109124 at 1/400 dilution (0.35 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Positive staining on the rat spleen.
The section was incubated with ab109124 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Secondary antibody only control: Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plastin L antibody [EPR4277] (ab109124)](https://www.abcam.cn/ps/products/109/ab109124/Images/ab109124-541699-anti-plastin-l-antibody-epr4277-immunohistochemistry-mouse-spleen.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plastin L antibody [EPR4277] (ab109124)Immunohistochemical analysis of paraffin-embedded Mouse spleen labelling Plastin L with ab109124 at 1/400 dilution (0.35 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Positive staining on the mouse spleen.
The section was incubated with ab109124 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Secondary antibody only control: Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plastin L antibody [EPR4277] (ab109124)](https://www.abcam.cn/ps/products/109/ab109124/Images/ab109124-541697-anti-plastin-l-antibody-epr4277-immunohistochemistry-mouse-colon-1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plastin L antibody [EPR4277] (ab109124)Immunohistochemical analysis of paraffin-embedded Mouse colon labelling Plastin L with ab109124 at 1/400 dilution (0.35 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Positive staining on the immune cells in mouse colon.
The section was incubated with ab109124 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Secondary antibody only control: Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
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![Western blot - Anti-Plastin L antibody [EPR4277] (ab109124)](https://www.abcam.cn/ps/products/109/ab109124/Images/ab109124-541694-anti-plastin-l-antibody-epr4277-western-blot-rat-brain.jpg)
Western blot - Anti-Plastin L antibody [EPR4277] (ab109124)All lanes : Anti-Plastin L antibody [EPR4277] (ab109124) at 1/1000 dilution
Lane 1 : Low expression control: Rat brain tissue lysate
Lane 2 : Low expression control: Rat liver tissue lysate
Lane 3 : Low expression control: Rat testis tissue lysate
Lane 4 : Rat spleen tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/200000 dilution
Predicted band size: 70 kDa
Observed band size: 70 kDa
Exposure time: 10 secondsBlocking and dilution buffer: 5% NFDM/TBST.
Low expression control: Rat brain, rat liver, rat testis.
In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution.
The lysates were freshly made and used for Western Blotting immediately to minimize protein degradation.
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![Western blot - Anti-Plastin L antibody [EPR4277] (ab109124)](https://www.abcam.cn/ps/products/109/ab109124/Images/ab109124-541693-anti-plastin-l-antibody-epr4277-western-blot-mouse-brain.jpg)
Western blot - Anti-Plastin L antibody [EPR4277] (ab109124)All lanes : Anti-Plastin L antibody [EPR4277] (ab109124) at 1/1000 dilution
Lane 1 : Low expression control: Mouse brain tissue lysate
Lane 2 : Low expression control: Mouse liver tissue lysate
Lane 3 : Low expression control: Mouse testis tissue lysate
Lane 4 : Mouse spleen tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/200000 dilution
Predicted band size: 70 kDa
Observed band size: 70 kDa
Exposure time: 10 secondsBlocking and dilution buffer: 5% NFDM/TBST.
Low expression control: mouse brain, mouse liver, mouse testis.
In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution.
The lysates were freshly made and used for Western Blotting immediately to minimize protein degradation.
-
![Western blot - Anti-Plastin L antibody [EPR4277] (ab109124)](https://www.abcam.cn/ps/products/109/ab109124/Images/ab109124-121717-anti-plastin-l-antibody-epr4277-western-blot.jpg)
Western blot - Anti-Plastin L antibody [EPR4277] (ab109124)All lanes : Anti-Plastin L antibody [EPR4277] (ab109124) at 1/1000 dilution
Lane 1 : THP1 cell lysates
Lane 2 : K562 cell lysates
Lane 3 : Jurkat cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled Goat anti-Rabbit at 1/2000 dilution
Predicted band size: 70 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plastin L antibody [EPR4277] (ab109124)](https://www.abcam.cn/ps/products/109/ab109124/Images/ab109124-121716-anti-plastin-l-antibody-epr4277-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plastin L antibody [EPR4277] (ab109124)ab109124, at 1/100 dilution staining Plastin L in paraffin-embedded Human tonsil tissue, by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Anti-Plastin L antibody [EPR4277] (ab109124)](https://www.abcam.cn/ps/products/109/ab109124/Images/ab109124-3-benefits-of-recombinant-antibodies.png)
Anti-Plastin L antibody [EPR4277] (ab109124)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab109124 被引用在 3 文献中.
- Li X et al. Targeting actin-bundling protein L-plastin as an anabolic therapy for bone loss. Sci Adv 6:N/A (2020). PubMed: 33208358
- Wang LL et al. Platelet-derived growth factor subunit B is required for tendon-bone healing using bone marrow-derived mesenchymal stem cells after rotator cuff repair in rats. J Cell Biochem 119:8897-8908 (2018). PubMed: 30105826
- Lapek JD et al. Defining Host Responses during Systemic Bacterial Infection through Construction of a Murine Organ Proteome Atlas. Cell Syst 6:579-592.e4 (2018). PubMed: 29778837