为了使您在Abcam官网的浏览体验更顺畅,请使用最新版本的浏览器比如 Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
致电 +86 21 2070 0500 或 联系我们

  • 我的账户
  • 退出
登录 或 注册

欢迎光临

登录 或

您还没有帐户?

注册
我的购物篮
快速订购
Abcam homepage

  • 研究用途的产品
    按产品类型
    一抗
    二抗
    免疫测定试剂盒与试剂
    细胞与组织成像工具
    细胞与生化测定
    蛋白质与肽
    按产品类型
    蛋白质组学工具
    激动剂、活化剂、拮抗剂与抑制剂
    细胞株和裂解液
    多重miRNA检测
    多因子分析试剂盒
    按研究领域
    癌症
    心血管
    细胞生物学
    表观遗传学
    代谢
    发育生物学
    按研究领域
    免疫学
    微生物学
    神经科学
    信号转导
    干细胞
  • 定制化产品 & 合作关系
    定制化产品 & 合作关系

    定制化产品和商务合作加速您的诊断和治疗项目

    定制化产品

    与我们成为伙伴

  • 技术支持
    支持中心

    获取研究建议和支持

    查看支持中心

    Protocols

    一步一步完成您的实验

    查看Protocols

  • 活动通知
    • 会议年历
    • 肿瘤研究
    • 心血管研究
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 神经生物学
    • 干细胞研究
    • 商品展览
    • 实验技术视频
    查看最新活动

    活动说明、摘要提交、演讲者、注册方式及更多信息

    查看全球活动安排

  • 通路
    细胞信号转导通路

    查看所有通路

    查看交叉通路

Cardiovascular Blood Serum Proteins

Anti-Plasma Kallikrein 1B抗体[13G11] (ab1006)

  • Datasheet
Reviews (3)Q&A (1)References (10)

Product price, shipping and contact information

Currently unavailable

抱歉,我们目前无法显示此内容。

请联系我们以完成下单,或者之后再尝试访问。

 

正在加载规格和价格。。。

 

送货和订单信息

运输费用

Promotion Information

Abpromise

产品质量保证,客户专家支持,

点击获取更多

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plasma Kallikrein 1B antibody [13G11] (ab1006)

    Key features and details

    • Mouse monoclonal [13G11] to Plasma Kallikrein 1B
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG1

    你可能会感兴趣

    分析检测
    Product image
    Malate Dehydrogenase 2 (MDH2) Activity Assay (ab119693)
    一抗
    Product image
    Anti-ADAMTS13 antibody [EPR6132] (ab177940)
    二抗
    Product image
    Goat Anti-Mouse IgG H&L (HRP) (ab205719)

    查看更多相关产品

    概述

    • 产品名称

      Anti-Plasma Kallikrein 1B抗体[13G11]
      参阅全部 Plasma Kallikrein 1B 一抗
    • 描述

      小鼠单克隆抗体[13G11] to Plasma Kallikrein 1B
    • 宿主

      Mouse
    • 经测试应用

      适用于: IHC-Pmore details
    • 种属反应性

      与反应: Human
    • 免疫原

      Full length native protein (purified) corresponding to Human Plasma Kallikrein 1B.
      Database link: P03952.1

    • 表位

      The specific epitope, found on the prekallikrein / kallikrein heavy chain is involved in the interaction between prekallikrein and factor XIIa.
    • 常规说明

      This product was changed from ascites to tissue culture supernatant on 19/12/2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions please do not hesitate to contact our scientific support team.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • 存储溶液

      pH: 7.4
    • Concentration information loading...
    • 纯度

      Protein G purified
    • 纯化说明

      Purified from TCS
    • 克隆

      单克隆
    • 克隆编号

      13G11
    • 同种型

      IgG1
    • 研究领域

      • Cardiovascular
      • Blood
      • Serum Proteins
      • Cardiovascular
      • Blood
      • Coagulation
      • Intrinsic
      • Signal Transduction
      • Cytoskeleton / ECM
      • Extracellular Matrix
      • ECM Enzymes
      • Kallikreins
      • Cancer
      • Invasion/microenvironment
      • ECM
      • Extracellular matrix
      • Kallikreins
      • Cell Biology
      • Proteolysis / Ubiquitin
      • Proteolytic enzymes
      • Serine protease
      • Kallikreins
      • Cardiovascular
      • Blood
      • Blood Pressure regulation

    相关产品

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Isotype control

      • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Recombinant Protein

      • Recombinant Rat Plasma Kallikrein 1B protein (His tag) (ab226435)

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab1006于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    IHC-P
    Use a concentration of 4 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
    说明
    IHC-P
    Use a concentration of 4 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    靶标

    • 功能

      The enzyme cleaves Lys-Arg and Arg-Ser bonds. It activates, in a reciprocal reaction, factor XII after its binding to a negatively charged surface. It also releases bradykinin from HMW kininogen and may also play a role in the renin-angiotensin system by converting prorenin into renin.
    • 疾病相关

      Defects in KLKB1 are the cause of prekallikrein deficiency (PKK deficiency) [MIM:612423]; also known as Fletcher factor deficiency. This disorder is a blood coagulation defect.
    • 序列相似性

      Belongs to the peptidase S1 family. Plasma kallikrein subfamily.
      Contains 4 apple domains.
      Contains 1 peptidase S1 domain.
    • 细胞定位

      Secreted.
    • Target information above from: UniProt accession P03952 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 数据库链接

      • Entrez Gene: 3818 Human
      • Omim: 229000 Human
      • SwissProt: P03952 Human
      • Unigene: 237642 Human
      • 别名

        • Fletcher factor antibody
        • kallikrein B plasma antibody
        • Kallikrein B plasma (Fletcher factor) 1 antibody
        • Kallikrein B, plasma 1 antibody
        • Kallikrein B1 antibody
        • Kininogenin antibody
        • KLK3 antibody
        • KLKB1 antibody
        • KLKB1_HUMAN antibody
        • PKK antibody
        • PKKD antibody
        • Plasma kallikrein antibody
        • Plasma kallikrein B1 antibody
        • Plasma kallikrein light chain antibody
        • Plasma prekallikrein antibody
        • PPK antibody
        • Prekallikrein antibody
        see all

      图片

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plasma Kallikrein 1B antibody [13G11] (ab1006)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plasma Kallikrein 1B antibody [13G11] (ab1006)

        ab1006 (4µg/ml) staining plasma kallikrein in human liver, using an automated system (DAKO Autostainer Plus). Using this protocol there is weak cytoplasmic staining of a subpopulation of hepatocytes.
        Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

        This image was generated using the ascites version of the product. 

      实验方案

      • Immunohistochemistry protocols

      Click here to view the general protocols

      数据表及文件

      • Datasheet download

        Download

      文献 (10)

      发表研究结果有使用 ab1006?请让我们知道,以便我们可以引用本数据表中的参考文章。

      ab1006 被引用在 10 文献中.

      • Song T  et al. Integrated Proteomics and Metabolomic Analyses of Plasma Injury Biomarkers in a Serious Brain Trauma Model in Rats. Int J Mol Sci 20:N/A (2019). PubMed: 30791599
      • Zamolodchikov D  et al. A Short Isoform of Coagulation Factor XII mRNA Is Expressed by Neurons in the Human Brain. Neuroscience 413:294-307 (2019). PubMed: 31181367
      • Badawy AA  et al. Expression of Epidermal Growth Factor Receptor and Transforming Growth Factor Alpha in Cancer Bladder: Schistosomal and Non-Schistosomal. Curr Urol 9:192-201 (2017). PubMed: 28413380
      • Liu J  et al. Hyperglycemia-induced cerebral hematoma expansion is mediated by plasma kallikrein. Nat Med 17:206-10 (2011). Functional Studies ; Rat . PubMed: 21258336
      • Morris KE  et al. Autonomic nervous system regulates secretion of anti-inflammatory prohormone SMR1 from rat salivary glands. Am J Physiol Cell Physiol 296:C514-24 (2009). WB ; Rat . PubMed: 19109528
      • Gao BB  et al. Extracellular carbonic anhydrase mediates hemorrhagic retinal and cerebral vascular permeability through prekallikrein activation. Nat Med 13:181-8 (2007). PubMed: 17259996
      • Veloso D Does resistance to endotoxin in primates correlate with levels of plasma prekallikrein? Immunopharmacology 33:374-6 (1996). PubMed: 8856190
      • Veloso D  et al. Structure, kinetics, and function of human and rhesus plasma prekallikreins are similar. Thromb Haemost 68:526-33 (1992). PubMed: 1455399
      • Veloso D & Colman RW Western blot analyses of prekallikrein and its activation products in human plasma. Thromb Haemost 65:382-8 (1991). PubMed: 1711719
      • Veloso D  et al. A monoclonal anti-human plasma prekallikrein antibody that inhibits activation of prekallikrein by factor XIIa on a surface. Blood 70:1053-62 (1987). PubMed: 2443201

      客户评价及客户问答

      Show All 评价 Q&A
      提交评价 提交问题

      1-4 of 4 Abreviews or Q&A

      Western blot abreview for Anti-Plasma Kallikrein 1B antibody [13G11]

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Purified protein (human plasma)
      Loading amount
      3 µg
      Specification
      human plasma
      Gel Running Conditions
      Reduced Denaturing
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 Dec 31 2009

      Western blot abreview for Anti-Plasma Kallikrein 1B antibody [13G11]

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Rat Tissue lysate - whole (Retina)
      Loading amount
      30 µg
      Specification
      Retina
      Gel Running Conditions
      Reduced Denaturing (10%)
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 Dec 31 2008

      Western blot abreview for Anti-Plasma Kallikrein 1B antibody [13G11]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Tissue lysate - other (Human Vitreous)
      Specification
      Human Vitreous
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 Feb 12 2007

      Question

      Prekallikrein / Kallikrein antibody (ab1006) [13G11] Is it tissue Kallikerin, PSA or others?

      Read More

      Abcam community

      Verified customer

      Asked on Oct 13 2003

      Answer

      Unfortunately, we do not have more information about this antibody. We will update the on-line datasheet of this product as soon as we get more data. We apologize for any inconvenience caused.

      Read More

      Abcam Scientific Support

      回复于 Oct 14 2003

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

      通过邮件收到学术资源和促销信息 注册
      研究类别(A-Z)
      • 肿瘤研究
      • 心血管研究
      • 细胞生物学
      • 发育生物学
      • 染色质及细胞核信号转导研究
      • 免疫学
      • 代谢
      • 微生物学
      • 神经生物学
      • 信号转导研究
      • 干细胞研究
      产品种类(A-Z)
      • 一抗
      • 二抗
      • 生化试剂
      • 亚类对照
      • 流式细胞仪多色选择器
      • 试剂盒
      • 内参对照
      • 细胞裂解液
      • 多肽
      • 蛋白酶
      • 组织切片
      • 蛋白标签及细胞标记物
      • 相关试剂及工具
      帮助与支持
      • 技术支持
      • 技术FAQ
      • 实验步骤与建议
      • 购买FAQ
      • RabMAb 产品
      • 培训课程
      • 按靶标浏览
      企业
      • 企业资讯
      • 投资者关系
      • 公司新闻
      • 招贤纳士
      • 关于我们
      活动通知
      • 商品展览
      • 会议
      全球网站
      • abcam.com
      • abcam.co.jp
      QR code for Weechat

      微信扫码 关注我们

      沪ICP备14046373号-1

      沪公网安备 31011502007413号

      • 销售条款
      • 网站使用条款
      • Cookie政策
      • 隐私权政策
      • 法律
      © 1998-2023 艾博抗(上海)贸易有限公司版权所有