重组Anti-PLAGL1 / ZAC抗体[EPR7523] (ab129063)
![Western blot - Anti-PLAGL1 / ZAC antibody [EPR7523] (ab129063)](https://www.abcam.cn/ps/products/129/ab129063/Images/ab129063-142996-anti-plagl1-zac-antibody-epr7523-western-blot.jpg "Western blot - Anti-PLAGL1 / ZAC antibody [EPR7523] (ab129063)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7523] to PLAGL1 / ZAC
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-PLAGL1 / ZAC抗体[EPR7523]
参阅全部 PLAGL1 / ZAC 一抗 -
描述
兔单克隆抗体[EPR7523] to PLAGL1 / ZAC -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WBmore details
不适用于: ICC/IF,IHC-P or IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human PLAGL1/ ZAC aa 400 to the C-terminus (C terminal). The exact sequence is proprietary.
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阳性对照
- Human fetal liver and Human fetal kidney, 293T, HepG2 and SH-SY5Y lysates. Permeabilized HepG2 cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数（KD）
KD = 1.71 x 10 -10 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR7523 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab129063于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB |
1/10000 - 1/50000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).
|
| 说明 |
|---|
|
Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/10000 - 1/50000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa). |
靶标
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功能
Shows weak transcriptional activatory activity. Transcriptional regulator of the type 1 receptor for pituitary adenylate cyclase-activating polypeptide. -
序列相似性
Belongs to the krueppel C2H2-type zinc-finger protein family.
Contains 7 C2H2-type zinc fingers. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 5325 Human
- Omim: 603044 Human
- SwissProt: Q9UM63 Human
- Unigene: 725973 Human
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别名
- DKFZp781P1017 antibody
- Lost on transformation 1 antibody
- Lost on Transformation antibody
see all
图片
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Western blot - Anti-PLAGL1 / ZAC antibody [EPR7523] (ab129063)All lanes : Anti-PLAGL1 / ZAC antibody [EPR7523] (ab129063) at 1/10000 dilution
Lane 1 : Human fetal liver tissue lysate
Lane 2 : Human fetal kidney tissue lysate
Lane 3 : 293T (Human embryonic kidney epithelial cell) cell lysate
Lane 4 : HepG2 cell lysate
Lane 5 : SH-SY5Y cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 51 kDa
Observed band size: 51 kDa -
![Flow Cytometry (Intracellular) - Anti-PLAGL1 / ZAC antibody [EPR7523] (ab129063)](https://www.abcam.cn/ps/products/129/ab129063/Images/ab129063-142995-anti-plagl1-zac-antibody-epr7523-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-PLAGL1 / ZAC antibody [EPR7523] (ab129063)ab129063 at 1/10 dilution staining PLAGL1 / ZAC in permeabilized HepG2 cells by intracellular flow cytometry (red) or a rabbit IgG negative (green).
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![OI-RD Scanning - Anti-PLAGL1 / ZAC antibody [EPR7523] (ab129063)](https://www.abcam.cn/ps/products/129/ab129063/Images/ab129063-142994-anti-plagl1-zac-antibody-epr7523.jpg)
OI-RD Scanning - Anti-PLAGL1 / ZAC antibody [EPR7523] (ab129063) -
![Anti-PLAGL1 / ZAC antibody [EPR7523] (ab129063)](https://www.abcam.cn/ps/products/129/ab129063/Images/ab129063-4-benefits-of-recombinant-antibodies.png)
Anti-PLAGL1 / ZAC antibody [EPR7523] (ab129063)
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab129063 被引用在 5 文献中.
- Chu YW et al. The cytotoxic mechanism of epigallocatechin gallate on proliferative HaCaT keratinocytes. J Biomed Sci 24:55 (2017). PubMed: 28810862
- Godlewski J et al. PLAGL1 (ZAC1/LOT1) Expression in Clear Cell Renal Cell Carcinoma: Correlations with Disease Progression and Unfavorable Prognosis. Anticancer Res 36:617-24 (2016). PubMed: 26851016
- Godlewski J et al. PLAGL1 protein is differentially expressed in the nephron segments and collecting ducts in human kidney. Folia Histochem Cytobiol 53:96-104 (2015). PubMed: 25823562
- Kowalczyk AE et al. Altered expression of the PLAGL1 (ZAC1/LOT1) gene in colorectal cancer: Correlations to the clinicopathological parameters. Int J Oncol 47:951-62 (2015). PubMed: 26134521
- Iglesias-Platas I et al. Altered expression of the imprinted transcription factor PLAGL1 deregulates a network of genes in the human IUGR placenta. Hum Mol Genet N/A:N/A (2014). PubMed: 24993786