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Tags & Cell Markers Fusion / Marker Proteins Alkaline Phosphatase

Anti-Placental alkaline phosphatase (PLAP)抗体[8B6] (ab33)

  • Datasheet
Submit a review Q&A (8)References (6)

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Western blot - Anti-Placental alkaline phosphatase (PLAP) antibody [8B6] (ab33)

    Key features and details

    • Mouse monoclonal [8B6] to Placental alkaline phosphatase (PLAP)
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG2a

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    概述

    • 产品名称

      Anti-Placental alkaline phosphatase (PLAP)抗体[8B6]
      参阅全部 Placental alkaline phosphatase (PLAP) 一抗
    • 描述

      小鼠单克隆抗体[8B6] to Placental alkaline phosphatase (PLAP)
    • 宿主

      Mouse
    • 经测试应用

      适用于: WBmore details
    • 种属反应性

      与反应: Human
    • 免疫原

      Tissue, cells or virus corresponding to Placental alkaline phosphatase (PLAP). Fresh Hep-2 cells followed by booster injections of fresh Hep-2 cells that have had their surface expression of PLAP boosted in culture.

    • 阳性对照

      • WB: Human placenta tissue lysate.
    • 常规说明

      This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
    • 存储溶液

      Constituent: 0.02% PBS
    • Concentration information loading...
    • 纯度

      Protein G purified
    • 克隆

      单克隆
    • 克隆编号

      8B6
    • 骨髓瘤

      P3-NS1/1-Ag4-1
    • 同种型

      IgG2a
    • 轻链类型

      kappa
    • 研究领域

      • Tags & Cell Markers
      • Fusion / Marker Proteins
      • Alkaline Phosphatase

    相关产品

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Isotype control

      • Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)
    • Recombinant Protein

      • Recombinant Human Placental alkaline phosphatase (PLAP) protein (ab191941)

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab33于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    WB
    Use a concentration of 5 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 58 kDa).
    说明
    WB
    Use a concentration of 5 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 58 kDa).

    靶标

    • 序列相似性

      Belongs to the alkaline phosphatase family.
    • 细胞定位

      Cell membrane.
    • Target information above from: UniProt accession P05187 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 数据库链接

      • Entrez Gene: 250 Human
      • Omim: 171800 Human
      • SwissProt: P05187 Human
      • Unigene: 284255 Human
      • 别名

        • Alkaline phosphatase antibody
        • Alkaline phosphatase placental antibody
        • Alkaline phosphatase placental type antibody
        • Alkaline phosphatase Regan isozyme antibody
        • ALP antibody
        • Alp1 antibody
        • ALPP antibody
        • FLJ61142 antibody
        • Germ-cell alkaline phosphatase antibody
        • nagao isozyme antibody
        • OTTHUMP00000164354 antibody
        • PALP antibody
        • Placental alkaline phosphatase 1 antibody
        • placental heat-stable alkaline phosphatase antibody
        • placental type antibody
        • PLAP antibody
        • PLAP-1 antibody
        • PLAP1 antibody
        • PPB1_HUMAN antibody
        see all

      图片

      • Western blot - Anti-Placental alkaline phosphatase (PLAP) antibody [8B6] (ab33)
        Western blot - Anti-Placental alkaline phosphatase (PLAP) antibody [8B6] (ab33)
        All lanes : Anti-Placental alkaline phosphatase (PLAP) antibody [8B6] (ab33) at 5 µg/ml

        Lane 1 : Human placenta tissue lysate
        Lane 2 : Human kidney tissue lysate (negative control)
        Lane 3 : Human liver tissue lysate (negative control)

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution

        Predicted band size: 58 kDa
        Observed band size: 65 kDa why is the actual band size different from the predicted?


        Exposure time: 8 minutes


        Blocking buffer: 3% milk.

        Gel Type: MOPS.

      实验方案

      • Immunohistochemistry protocols

      Click here to view the general protocols

      数据表及文件

      • Datasheet download

        Download

      文献 (6)

      发表研究结果有使用 ab33?请让我们知道,以便我们可以引用本数据表中的参考文章。

      ab33 被引用在 6 文献中.

      • Hu Y  et al. High-Mobility Group Box 1 From Hypoxic Trophoblasts Promotes Endothelial Microparticle Production and Thrombophilia in Preeclampsia. Arterioscler Thromb Vasc Biol 38:1381-1391 (2018). PubMed: 29650693
      • Müller WEG  et al. Polyphosphate as a donor of high-energy phosphate for the synthesis of ADP and ATP. J Cell Sci 130:2747-2756 (2017). PubMed: 28687622
      • Sharp TM  et al. Inhibition of cellular protein secretion by norwalk virus nonstructural protein p22 requires a mimic of an endoplasmic reticulum export signal. PLoS One 5:e13130 (2010). ICC/IF ; Human . PubMed: 20976190
      • Zhang L  et al. Alteration in the IL-2 signal peptide affects secretion of proteins in vitro and in vivo. J Gene Med 7:354-65 (2005). ELISA ; Human . PubMed: 15619290
      • Durbin H  et al. Monoclonal antibodies to placental alkaline phosphatase: preclinical evaluation in a human xenograft tumour model of F(ab')2 and Fab fragments. Int J Cancer Suppl 2:59-66 (1988). PubMed: 3162446
      • Durbin H  et al. Production of monoclonal antibodies to placental alkaline phosphatase: preliminary characterisation includes identification of one antibody reactive with routinely fixed histological preparations. Int J Cancer Suppl 2:50-8 (1988). PubMed: 2450848

      客户评价及客户问答

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      1-8 of 8 Abreviews or Q&A

      Question

      Our lab is considering the antibody "Anti-placental alkaline phosphatase" with catalog #ab33.   The datasheet of this antibody indicates that the cross reactivity is "reacts with Human". We want to know if it can also reacts with mouse. I want to look for assistance if your techsupport have any information that any PLAP antibody can also work on mouse brain tissue or mouse brain primary culture?

      Read More

      Abcam community

      Verified customer

      Asked on Dec 27 2012

      Answer

      Thank you for your enquiry. Unfortunately we have not tested ab33 against mouse so we do not know whether it is suitable. The antibody was raised against the whole protein, and although the mouse and human protein show 75% homology we do not know which part of the protein it is reactive against and thus the exact homology in mouse. We do stock another antibody which is reactive in IHC-P on mouse samples, which would be more suitable: ab16695 - Anti-Placental alkaline phosphatase (PLAP) antibody [SP15] IHC-P, WB Reacts with Mouse and Human It has not been tested in ICC on primary cultures, but if you are interested in testing it as part of our https://www.abcam.com/abtrial program, just let me know. I hope this information helps.

      Read More

      Abcam Scientific Support

      回复于 Dec 27 2012

      Question

      I have a customer enquiry regarding ab33 (PLAP protein). She would like to know the expected molecular weight of the protein, she also wants to test the antibody for western blot. Although it is not on the datasheet as a tested application, she was wondering if Abcam could suggest a dilution. Thank you for your time and I look forward to your reply.

      Read More

      Abcam community

      Verified customer

      Asked on Oct 17 2005

      Answer

      Thank you for your enquiry. According to the database SwissProt the MW of the precursor PLAP is 57kDa and it is glycosylated therefore its weight should be higher than this. A paper by Webb et al (Placenta. 1986 Sep-Oct;7(5):405-15.Isolation of placental-type alkaline phosphatase associated with human syncytiotrophoblast membranes using monoclonal antibodies) describes "an eluted enzymatically active PLAP had a molecular weight in the regions of 115K and 130K, with an occasional higher molecular weight form at 180K. On reduction to a 66K monomeric form". Regarding dilutions to try, I would recommend trying a range of dilutions in a preliminary experiment (1:250, 1:500, 1:1000) with overnight incubation and optimising following the results of that experiment. If the customer decides to go ahead and purchase this product, please ask him/her to let us know how he/she gets on by submitting an Abreview and in return we will award him/her 50 Abcam Points, which can be redeemed on a number of rewards (a further 100 Abcam Points will be offered for an image). Please let me know if you require further information,

      Read More

      Abcam Scientific Support

      回复于 Oct 17 2005

      Question

      About a year ago someone asked if the PLAP 8B6-ab33 antibody could be used in westernblotting. At that time you said it hasn't been tested for that yet. Has it been tested since, and can it be used in westernblotting? Mark

      Read More

      Abcam community

      Verified customer

      Asked on May 28 2002

      Answer

      I'm afraid that we do not have any data regarding the suitablity of this antibody for WB.

      Read More

      Abcam Scientific Support

      回复于 May 29 2002

      Question

      Is this antibody suitable for flow cytometry?

      Read More

      Abcam community

      Verified customer

      Asked on Oct 10 2001

      Answer

      This antibody has not been tested in flow cytometry.

      Read More

      Abcam Scientific Support

      回复于 Oct 11 2001

      Question

      Marseille 1 october 2001 dear sir, ab33 is an anti human alkaline phosphatase, I would like to know if this antibody is suitable for immunoflorescence in cell cultures. Thank you very much John-Baptist

      Read More

      Abcam community

      Verified customer

      Asked on Oct 01 2001

      Answer

      This antibody has not been tested in immunofluorescence.

      Read More

      Abcam Scientific Support

      回复于 Oct 02 2001

      Question

      Hi, can you please tell me what epitope ab33 was raised against, please? I am trying to detect a fusion protein which has the AP sequence from nucleotides 32 to 1708. If the answer is proprietary, please let me know if it will detect a protein within the translating regions of those nucleotides. The GenBank accession number is M68513. Thank you very much, Jerry

      Read More

      Abcam community

      Verified customer

      Asked on Sep 20 2001

      Answer

      This monoclonal was raised against the whole protein, and in the absence of any epitope mapping studies, I am afraid that we do not know against which part of the protein the antibody is reactive.

      Read More

      Abcam Scientific Support

      回复于 Sep 24 2001

      Question

      Could you let me know whether antibodies against alkaline phospatase from human placenta inhibitor not the activity of alkaline phosphatase itself in solution or on the surface of the membrane? Thank you. Vlad

      Read More

      Abcam community

      Verified customer

      Asked on Aug 07 2001

      Answer

      Vlad We do not have any data on this antibody's inhibiting activity in vivo or in vitro. We are about to implement a trial 'try risk free' scheme which says that if an antibody is purchased and used in an experiment outside that written on the datasheet, and it is found not to work, in return for the negative data (which we can add to the datasheet to help other researchers) we will refund or issue a credit note.

      Read More

      Abcam Scientific Support

      回复于 Aug 07 2001

      Question

      I was wondering if this antibody (alkaline phosphatase 8B6) can be used for Western Blotting? It's not listed as one of the applications.

      Read More

      Abcam community

      Verified customer

      Asked on Jul 10 2001

      Answer

      This antibody has not yet been tested in Western blotting.

      Read More

      Neil Hayward

      Abcam Scientific Support

      回复于 Jul 13 2001

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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