AB221738

Anti-PKMYT1抗体

Name: PKMYT1抗体 (ab221738)| Abcam中文官网
Brand: Abcam Limited
SKU: AB221738
Price: 5757 CNY
Availability: InStock

Anti-PKMYT1 antibody

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(1 Publication)

Rabbit Polyclonal PKMYT1 antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human PKMYT1 aa 400-500.

查看别名

MYT1, PKMYT1, Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase, Myt1 kinase

1 Images

Immunocytochemistry/ Immunofluorescence - Anti-PKMYT1 antibody (AB221738)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PKMYT1 antibody (AB221738)

PFA-fixed, Triton X-100 permeabilized MCF7 (human breast adenocarcinoma cell line) cells stained for PKMYT1 (green) using ab221738 at 4 μg/ml in ICC/IF.

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Human

应用

ICC/IF

applications

免疫原

Recombinant Fragment Protein within Human PKMYT1 aa 400-500. The exact immunogen used to generate this antibody is proprietary information.

Q99640

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

存储溶液

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

PKMYT1 also known as Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase is an important regulator of the cell cycle. It is a protein kinase with a molecular mass of about 62 kDa and is expressed in various tissues including the brain and reproductive organs. PKMYT1 has a role in regulating the entry into the mitotic phase by inhibiting the Cyclin-dependent kinase 1 (CDK1) which controls the progression through different phases of the cell cycle.

Biological function summary

This kinase is involved in controlling cell division essential for maintaining genomic integrity. PKMYT1 although not always a direct part of a physical complex significantly impacts the activation of CDK1-cyclin B complex which initiates mitosis. By phosphorylating the CDK1 complex PKMYT1 ensures proper timing of cell cycle transitions preventing premature or erroneous mitotic entry.

Pathways

PKMYT1 involvement is notable in the cell cycle regulation specifically during the G2/M transition. PKMYT1 phosphorylates CDK1 inhibiting its activity until the cell is ready to progress into mitosis. Related proteins in this pathway include CDC25 a phosphatase that removes the inhibitory phosphate groups from CDK1 allowing progression to mitosis. Through these interactions PKMYT1 tightly regulates the commitment to mitosis ensuring cells divide accurately.

Studies have linked PKMYT1 to various types of cancer due to its role in cell cycle control. Aberrations in PKMYT1 activity can lead to uncontrolled cell proliferation seen in cancers such as glioblastoma and ovarian cancer. PKMYT1 through its interactions with CDK1 and other cell cycle regulators has potential therapeutic implications. Inhibitors targeting PKMYT1 might help control tumor growth by restoring proper cell cycle regulation serving as a strategic component in cancer therapy.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Acts as a negative regulator of entry into mitosis (G2 to M transition) by phosphorylation of the CDK1 kinase specifically when CDK1 is complexed to cyclins (PubMed : 10373560, PubMed : 10504341, PubMed : 9001210, PubMed : 9268380). Mediates phosphorylation of CDK1 predominantly on 'Thr-14'. Also involved in Golgi fragmentation (PubMed : 9001210, PubMed : 9268380). May be involved in phosphorylation of CDK1 on 'Tyr-15' to a lesser degree, however tyrosine kinase activity is unclear and may be indirect (PubMed : 9001210, PubMed : 9268380).

See full target information PKMYT1

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Experimental and therapeutic medicine 19:313-318 PubMed31853305

2019

A large-scale collection of giant congenital melanocytic nevi: Clinical and histopathological characteristics.

Applications

Unspecified application

Species

Unspecified reactive species

Min Wu,Qingxiong Yu,Bowen Gao,Lingling Sheng,Qingfeng Li,Feng Xie

View all publications

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Anti-PKMYT1抗体

Anti-PKMYT1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-PKMYT1 antibody (AB221738)