Anti-PKM抗体(ab137852)
Key features and details
- Rabbit polyclonal to PKM
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-PKM抗体
参阅全部 PKM 一抗 -
描述
兔多克隆抗体to PKM -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Recombinant fragment within Human PKM aa 1-250. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. The exact immunogen sequence is within the center region.
Database link: P14618 -
阳性对照
- WB: 293T, A431, HeLa. IHC-P: DLD1 xenograft tissue; human lung tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab137852于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
1/500 - 1/3000. Predicted molecular weight: 58 kDa.
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| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Please see protocol details in the legend for IHC-P on human lung tissue. |
| 说明 |
|---|
|
WB
1/500 - 1/3000. Predicted molecular weight: 58 kDa. |
|
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Please see protocol details in the legend for IHC-P on human lung tissue. |
靶标
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功能
Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival. -
组织特异性
Specifically expressed in proliferating cells, such as embryonic stem cells, embryonic carcinoma cells, as well as cancer cells. -
通路
Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5. -
序列相似性
Belongs to the pyruvate kinase family. -
翻译后修饰
ISGylated.
Under hypoxia, hydroxylated by EGLN3.
Acetylation at Lys-305 is stimulated by high glucose concentration, it decreases enzyme activity and promotes its lysosomal-dependent degradation via chaperone-mediated autophagy.
FGFR1-dependent tyrosine phosphorylation is reduced by interaction with TRIM35. -
细胞定位
Cytoplasm. Nucleus. Translocates to the nucleus in response to different apoptotic stimuli. Nuclear translocation is sufficient to induce cell death that is caspase independent, isoform-specific and independent of its enzymatic activity. - Information by UniProt
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数据库链接
- Entrez Gene: 5315 Human
- SwissProt: P14618 Human
- Unigene: 534770 Human
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别名
- CTHBP antibody
- Cytosolic thyroid hormone-binding protein antibody
- KPYM_HUMAN antibody
see all
图片
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Western blot - Anti-PKM antibody (ab137852)All lanes : Anti-PKM antibody (ab137852) at 1/500 dilution
Lane 1 : Non-transfected (–) 293T whole cell extracts
Lane 2 : 293T whole cell extracts transfected (+) with PKM shRNA
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Rabbit IgG antibody (HRP) at 1/10000 dilution
Predicted band size: 58 kDa7.5% SDS-PAGE.
Running conditions: 80V, 15min; 140V, 40 minutes.
Transfer condition: Semi-dry, 18 V, 60 min (NC membrane).
Blocking condition: 5% non-fat milk in TBST, RT, 60 minutes.
Primary antibody incubation: 4? , overnight.
Washing condition: 5 ml TBST, 4 x 5 minutes.
Exposure: chemiluminescent substrate for the detection of HRP-conjugated antibody
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKM antibody (ab137852)Paraffin-embedded human lung tissue stained for PKM using ab137852 at 1/500 dilution (4°C overnight) in immunohistochemical analysis.
Secondary antibody: ABC HRP Kit (Rabbit IgG) 1/200 at room temperature for 30 minutes.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 minutes. (Cuisinart Electric Pressure Cooker, choose "high pressure").
Endogenous peroxide blocking: 3% H2O2, RT, 30minutes.
Blocking condition: 1.5% goat serum (dilute goat serum by 1xPBS), RT, 30 minutes.
Washing condition: PBS, 2 x 5 minutes.
Chromogen system: DAB.
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Western blot - Anti-PKM antibody (ab137852)All lanes : Anti-PKM antibody (ab137852) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : H1299 whole cell lysate
Lane 4 : HeLa S3 whole cell lysate
Lane 5 : Hep G2 whole cell lysate
Lane 6 : MOLT4 whole cell lysate
Lane 7 : Raji whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Rabbit IgG antibody (HRP) at 1/10000 dilution
Predicted band size: 58 kDa10% SDS-PAGE.
Running conditions: 80V, 15min; 140V, 40 minutes.
Transfer condition: Semi-dry, 18 V, 60 min (NC membrane).
Blocking condition: 5% non-fat milk in TBST, RT, 60 minutes.
Primary antibody incubation: 4? , overnight.
Washing condition: 5 ml TBST, 4 x 5 minutes.
Exposure: enhanced ECL
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKM antibody (ab137852)Immunohistochemical analysis of paraffin-embedded PKM DLD1 xenograft tissue labelling PKM with ab137852 at 1/100 dilution.
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Western blot - Anti-PKM antibody (ab137852)Anti-PKM antibody (ab137852) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg
Predicted band size: 58 kDa
7.5% SDS PAGE -
Western blot - Anti-PKM antibody (ab137852)Anti-PKM antibody (ab137852) at 1/1000 dilution + Rat brain lysate/extract at 50 µg
Predicted band size: 58 kDa
7.5 % SDS-PAGE
数据表及文件
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SDS download
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Datasheet download
文献 (42)
ab137852 被引用在 42 文献中.
- Wu H et al. miR-140-3p/usp36 axis mediates ubiquitination to regulate PKM2 and suppressed the malignant biological behavior of breast cancer through Warburg effect. Cell Cycle 22:680-692 (2023). PubMed: 36305548
- Lv T et al. USP35 promotes hepatocellular carcinoma progression by protecting PKM2 from ubiquitination‑mediated degradation. Int J Oncol 63:N/A (2023). PubMed: 37594129
- Correia B et al. Leucine and Arginine Availability Modulate Mouse Embryonic Stem Cell Proliferation and Metabolism. Int J Mol Sci 23:N/A (2022). PubMed: 36430764
- Chen W et al. Exosomal circSHKBP1 participates in non-small cell lung cancer progression through PKM2-mediated glycolysis. Mol Ther Oncolytics 24:470-485 (2022). PubMed: 35229026
- Chen CJ et al. Metformin Mitigated Obesity-Driven Cancer Aggressiveness in Tumor-Bearing Mice. Int J Mol Sci 23:N/A (2022). PubMed: 36012397