Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online

Anti-PKD2抗体(ab7281)

Reviews (3)Q&A (1)References (4)

Key features and details

  • Rabbit polyclonal to PKD2
  • Suitable for: ICC/IF, IHC-P, IP, WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

选择批间可重复性更高的重组抗体

Product image
Anti-PKD2 antibody [EP1495Y] (ab51250)
  • 研究可靠 —— 各批次间结果一致且可重复
  • 长期批量供应 —— 采用重组技术，可实现快速生产
  • 首次实验即可成功 —— 经过大量验证确认了特异性
  • 符合伦理标准 —— 产品不含动物成分

概述

  • 产品名称

    Anti-PKD2抗体
    参阅全部 PKD2 一抗

  • 描述

    兔多克隆抗体to PKD2

  • 宿主

    Rabbit

  • 经测试应用

    适用于: ICC/IF, IHC-P, IP, WBmore details

  • 种属反应性

    与反应: Mouse, Human

  • 免疫原

    Synthetic peptide corresponding to Human PKD2 aa 849-864.

  • 常规说明

    This antibody recognizes human protein kinase D2 (PKD2), a serine threonine protein kinase. PKD2 is widely expressed in human and murine tissues and exhibits strong homology to the serine threonine protein kinases PKD/PKCmicro and PKCnu. In contrast, it has a low degree of sequence homology to other members of the PKC family. PKD2 is activated by phorbol esters and a variety of growth factors.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

  • 存储溶液

    pH: 7.4
    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • 纯度

    Immunogen affinity purified

  • Primary antibody说明

    This antibody recognizes human protein kinase D2 (PKD2), a serine threonine protein kinase. PKD2 is widely expressed in human and murine tissues and exhibits strong homology to the serine threonine protein kinases PKD/PKCmicro and PKCnu. In contrast, it has a low degree of sequence homology to other members of the PKC family. PKD2 is activated by phorbol esters and a variety of growth factors.

  • 克隆

    多克隆

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab7281于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF
Use at an assay dependent concentration.
IHC-P (1)
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
WB (2)
Use at an assay dependent concentration.
说明
ICC/IF
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.

靶标

  • 功能

    Converts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. Involved in resistance to oxidative stress.

  • 组织特异性

    Widely expressed.

  • 序列相似性

    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PKD subfamily.
    Contains 1 PH domain.
    Contains 2 phorbol-ester/DAG-type zinc fingers.
    Contains 1 protein kinase domain.

  • 翻译后修饰

    Phosphorylation of Ser-876 correlates with the activation status of the kinase.
    Ser-706 is probably phosphorylated by PKC.

  • 细胞定位

    Cytoplasm. Membrane. Translocation to the cell membrane is required for kinase activation.
  • Information by UniProt

  • 数据库链接

    • 别名

      • DKFZp586E0820 antibody
      • HSPC187 antibody
      • KPCD2_HUMAN antibody
      • nPKC D2 antibody
      • nPKC-D2 antibody
      • PKD2 antibody
      • PRKD 2 antibody
      • Prkd2 antibody
      • Protein kinase D2 antibody
      • Serine/threonine protein kinase D2 antibody
      • Serine/threonine-protein kinase D2 antibody
      see all

    文献 (4)

    发表研究结果有使用 ab7281？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab7281 被引用在 4 文献中.

    • Xiao Y  et al. Deficiency of PRKD2 triggers hyperinsulinemia and metabolic disorders. Nat Commun 9:2015 (2018). PubMed: 29789568
    • Arun SN  et al. Ultraviolet B irradiation and activation of protein kinase D in primary mouse epidermal keratinocytes. Oncogene : (2010). WB ; Mouse . PubMed: 21132013
    • Li J  et al. PKD1, PKD2, and Their Substrate Kidins220 Regulate Neurotensin Secretion in the BON Human Endocrine Cell Line. J Biol Chem 283:2614-21 (2008). ICC/IF ; Human . PubMed: 18048355
    • Jackson LN  et al. Overexpression of wild-type PKD2 leads to increased proliferation and invasion of BON endocrine cells. Biochem Biophys Res Commun 348:945-9 (2006). PubMed: 16899224

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-4 of 4 Abreviews or Q&A

    Western blot abreview for Anti-PKD2 antibody

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (NIH 3T3)
    Gel Running Conditions
    Reduced Denaturing (10%)
    Loading amount
    25 µg
    Specification
    NIH 3T3
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Feb 23 2010

    Western blot abreview for Anti-PKD2 antibody

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HMEC)
    Gel Running Conditions
    Reduced Denaturing (10%)
    Loading amount
    15 µg
    Specification
    HMEC
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Feb 22 2010

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Protein Kinase D2 antibody

     Excellent
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (placenta tissue-paraffin sections)
    Specification
    placenta tissue-paraffin sections
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    Heat mediated
    Blocking step
    Serum as blocking agent for 45 minute(s) · Concentration: 5%
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Daniel Macphee

    Verified customer

    提交于 Jan 30 2006

    Question

    I am using this antibody in mouse myoblasts. The antibody gives a distinct, clear band at the approximate expected size. However, I am interested in possible cross-reactivity with closely-related PKD1. Does this antibody cross-react with PKD1? What is the sequence of the immunogen that the antibody was raised against? Thank you.

    Read More
    Answer

    Thank you for your inquiry.

    This antibody was raised against the full length human Protein Kinase D2, plus it is a polyclonal, so the antibody will have multiple binding epitopes along the protein.

    I've BLASTed the immunogen against mouse Protein Kinase D1 and it shows 69% homology. Generally we say that anything with >85% sequence homology would be expected to cross-react, so this is quite low. However, we have never tested this antibody for its cross-reactivity with Protein Kinase D1 so we are unsure if it cross-reacts.

    I hope this information helps. Please contact us with any other questions.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com